Associations of Yeasts with Spotted-Wing Drosophila (Drosophila suzukii; Diptera: Drosophilidae) in Cherries and Raspberries

Hamby, Kelly A.; Hernández, Alejandro; Boundy‐Mills, Kyria; Zalom, Frank G.

doi:10.1128/aem.00841-12

Cited by 180 publications

(183 citation statements)

References 41 publications

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“…Inoculating culture media with dry baker's yeast further increased D. suzukii adult and pupal production by one-third. In two other studies, yeast augmentation increased D. suzukii oviposition and pupation by a similar rate (Jaramillo et al 2014;Hardin et al 2015), indicating yeasts are symbiotic and probably provide D. suzukii larvae with the protein that their natural habitats (e.g., ripe or ripening berries) lack (Hamby et al 2012;Hardin et al 2015). However, adding far more than 5 grains (>0.80 mg) of exogenous yeast to the diet could harm cultures if fermentation produces toxic levels of carbon dioxide that sterilize, incapacitate, or kill flies (Markow & O'Grady 2006).…”

Section: Discussionmentioning

confidence: 99%

“…However, adding far more than 5 grains (>0.80 mg) of exogenous yeast to the diet could harm cultures if fermentation produces toxic levels of carbon dioxide that sterilize, incapacitate, or kill flies (Markow & O'Grady 2006). Moreover, the diverse fungal community associated with D. suzukii is a possible source of endosymbionts that might someday be cultivated and introduced to a rearing system to enhance fly fitness or exploited in novel ways to reduce fly survival and reproduction (Hamby et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Triple rinsing berries with deionized or distilled water then removes or dilutes ethanol to a safe level and prevents the inadvertent poisoning of alcohol-sensitive adults (Sampson et al 2015). In their study of D. suzukii associated yeasts, Hamby et al (2012) pre-sterilized flies with 70% ethanol to remove any cuticular yeasts. Afterward, they rinsed away any residual alcohol that, if swallowed, could harm microbial endosymbionts.…”

Section: Discussionmentioning

confidence: 99%

“…Drosophila species typically vector a community of yeasts, of which many species, once introduced into host tissues, serve as vital sources of nutrients for ovipositing females and developing larvae. The presence of yeast, which can outcompete harmful microbes, can stimulate oviposition in many fruit-breeding species of Drosophila, and perhaps yeast benefits reproduction in D. suzukii (Hamby et al 2012). …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development, Reproductive Output and Population Growth of the Fruit Fly Pest <I>Drosophila suzukii</I> (Diptera: Drosophilidae) on Artificial Diet

Emiljanowicz¹,

Ryan²,

Langille³

et al. 2014

j econ entomol

114

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development, Reproductive Output and Population Growth of the Fruit Fly Pest <I>Drosophila suzukii</I> (Diptera: Drosophilidae) on Artificial Diet

Emiljanowicz¹,

Ryan²,

Langille³

et al. 2014

j econ entomol

114

View full text Add to dashboard Cite

“…In nature, Drosophila is associated primarily with yeasts in the phylum Ascomycota and the family Saccharomycetaceae (4,28,50,51,56). Different species of yeasts have different effects on Drosophila melanogaster survival and development time (2).…”

mentioning

confidence: 99%

Yeast Communities of Diverse Drosophila Species: Comparison of Two Symbiont Groups in the Same Hosts

Chandler

Eisen

Kopp

2012

Appl Environ Microbiol

149

163

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The combination of ecological diversity with genetic and experimental tractability makes Drosophila a powerful model for the study of animal-associated microbial communities. Despite the known importance of yeasts in Drosophila physiology, behavior, and fitness, most recent work has focused on Drosophila-bacterial interactions. In order to get a more complete understanding of the Drosophila microbiome, we characterized the yeast communities associated with different Drosophila species collected around the world. We focused on the phylum Ascomycota because it constitutes the vast majority of the Drosophila-associated yeasts. Our sampling strategy allowed us to compare the distribution and structure of the yeast and bacterial communities in the same host populations. We show that yeast communities are dominated by a small number of abundant taxa, that the same yeast lineages are associated with different host species and populations, and that host diet has a greater effect than host species on yeast community composition. These patterns closely parallel those observed in Drosophila bacterial communities. However, we do not detect a significant correlation between the yeast and bacterial communities of the same host populations. Comparative analysis of different symbiont groups provides a more comprehensive picture of host-microbe interactions. Future work on the role of symbiont communities in animal physiology, ecological adaptation, and evolution would benefit from a similarly holistic approach.

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Transmission of beneficial yeasts accompanies offspring production in Drosophila—An initial evolutionary stage of insect maternal care through manipulation of microbial load?

Cho

Rohlfs

2023

Ecology and Evolution

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Parent‐to‐offspring transmission of beneficial microorganisms is intimately interwoven with the evolution of social behaviors. Ancestral stages of complex sociality–microbe vectoring interrelationships may be characterized by high costs of intensive parental care and hence only a weak link between the transmission of microbial symbionts and offspring production. We investigate the relationship between yeast symbiont transmission and egg‐laying, as well as some general factors thought to drive the “farming” of microscopic fungi by the fruit fly Drosophila melanogaster, an insect with no obvious parental care but which is highly dependent on dietary microbes during offspring development. The process of transmitting microbes involves flies ingesting microbes from their previous environment, storing and vectoring them, and finally depositing them to a new environment. This study revealed that fecal materials of adult flies play a significant role in this process, as they contain viable yeast cells that support larval development. During single patch visits, egg‐laying female flies transmitted more yeast cells than non‐egg‐laying females, suggesting that dietary symbiont transmission is not random, but linked to offspring production. The crop, an extension of the foregut, was identified as an organ capable of storing viable yeast cells during travel between egg‐laying sites. However, the amount of yeast in the crop reduced rapidly during periods of starvation. Although females starved for 24 h deposited a smaller amount of yeast than those starved for 6 h, the yeast inoculum produced still promoted the development of larval offspring. The results of these experiments suggest that female Drosophila fruit flies have the ability to store and regulate the transfer of microorganisms beneficial to their offspring via the shedding of fecal material. We argue that our observation may represent an initial evolutionary stage of maternal care through the manipulation of microbial load, from which more specialized feedbacks of sociality and microbe management may evolve.

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Associations of Yeasts with Spotted-Wing Drosophila (Drosophila suzukii; Diptera: Drosophilidae) in Cherries and Raspberries

Cited by 180 publications

References 41 publications

Development, Reproductive Output and Population Growth of the Fruit Fly Pest <I>Drosophila suzukii</I> (Diptera: Drosophilidae) on Artificial Diet

Development, Reproductive Output and Population Growth of the Fruit Fly Pest <I>Drosophila suzukii</I> (Diptera: Drosophilidae) on Artificial Diet

Yeast Communities of Diverse Drosophila Species: Comparison of Two Symbiont Groups in the Same Hosts

Transmission of beneficial yeasts accompanies offspring production in Drosophila—An initial evolutionary stage of insect maternal care through manipulation of microbial load?

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