Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome

Abstract

BackgroundMale factor infertility is quite common as 30–50% of infertility cases are due to sperm defects. The high sperm DNA fragmentation is one of the causes of male factor infertility. Many factors cause sperm DNA fragmentation and could be testicular or post-testicular. The purpose of this study was to assess relationships among sperm DNA fragmentation, lifestyle factors and semen values of Saudi men and to determine impact of sperm DNA fragmentation on ICSI cycle outcome.MethodsThe duration of this study was from January 2015 to June 2016. The cases with female factor infertility were excluded. In total 94 couples were selected for investigation. The study parameters were male age, body mass index, smoking, semen values, % sperm DNA fragmentation, fertilization rate and pregnancy outcome. The ICSI procedure was performed in all patients per standard protocol. The semen samples were grouped based on % sperm DNA fragmentation into < 15%, 15–30 and > 30% which corresponded to low, moderate and high sperm DNA fragmentation, respectively.ResultsThere was no difference in ICSI outcome in low and moderate sperm DNA fragmentation, however, in high sperm DNA fragmentation no patient achieved pregnancy. In this study, 53.19% Saudi men had low, 32.98% moderate and 13.83% high DFI. Semen volume, sperm morphology and fertilization rate did not show any correlation trend with DNA fragmentation, however, sperm concentration and motility were negatively correlated in all DFI categories. The BMI was positively correlated in moderate DFI category and smoking was positively correlated with low DFI category. The age was positively correlated in moderate and high DFI categories.ConclusionsThe results of this study indicated that 14% Saudi men had high DNA fragmentation. The BMI was positively correlated in moderate DFI category and smoking was positively correlated with low DFI category. The age was positively correlated in moderate and high DFI categories.

Sperm DNA fragmentation index influences assisted reproductive technology outcome: A systematic review and meta‐analysis combined with a retrospective cohort study

“…Studies of the rate of good‐quality embryos included eight studies comprising 17,879 embryos (Chi et al, ; Fang, Lou, Ye, Jin, & Zhou, ; Jiang, He, Wang et al, ; Jiang et al, ; Niu et al, ; Oleszczuk et al, ; Xue et al, ). Studies of the clinical pregnancy rate included 23 studies comprising 6,771 cycles (Al Omrani et al, ; Anifandis et al, ; Boe‐Hansen et al, ; Bungum et al, ; Chi et al, ; Esbert et al, ; Fang et al, ; Gandini et al, ; Jiang, He, Wang et al, ; Jiang et al, ; Lin et al, ; Miciński, Pawlicki, Wielgus, Bochenek, & Tworkowska, ; Ni et al, ; Niu et al, ; Oleszczuk et al, ; Simon et al, ; Sivanarayana et al, ; Speyer et al, ; Wang et al, ; Xue et al, ; Yang et al, ; Zheng et al, ).…”

Effect of seminal redox status on lipid peroxidation, apoptosis and DNA fragmentation in spermatozoa of infertile Saudi males

“…34 The inverse correlated of Sperm DNA fragmentation with the total motility and normal morphology of spermatozoa, could be the reason of subfertility in the patient groups. [36][37][38] Significantly positive correlation recorded between seminal OS, LPO and DNA fragmentation in our study suggests that the observed LPO and DNA damage may have been due to increased ROS production. The high susceptibility of spermatozoa to OS-induced DNA damage can result not only in poor fertilization rates but also lead to poor embryo quality which can cause birth defects and even childhood diseases.…”

Beyond Acephalic Spermatozoa: The Complexity of Intracytoplasmic Sperm Injection Outcomes

“…Elkhatib et al [42] pointed out that assisted reproductive technology, such as ICSI methods, provides a therapeutic solution for almost all infertile couples with AS defects. Based on an ICSI protocol, sperm showing an intact morphology may be chosen to fertilise eggs, in case genetic material may be lost during head-tail separation [13,43]. erefore, in the most reported AS cases, an abnormal head-tail junction sperm was used during an ICSI procedure instead of a tailless head of sperm alone.…”

“…e natural fertilising abilities of these genes are lost because almost all AS sperm are broken in two. Intracytoplasmic sperm injection (ICSI) is the only technique so far in which abnormal or immotile sperm have the opportunity to fertilise oocytes successfully [13,14]. Meanwhile, Sha et al [11] believed that ICSI was the only solution to AS defects; however, not all AS-deficient sperm achieved the same clinical results [15].…”