Associations of serum expressions of miR-499 and sex determining region Y-box 6 with prognosis of acute myocardial infarction patients

Abstract

Background: To explore the associations of serum expressions of miR-499 and sex determining region Y-box 6 (SOX6) with major adverse cardiovascular and cerebrovascular events (MACCE) and prognosis of acute myocardial infarction (AMI) patients undergoing percutaneous coronary intervention (PCI). Methods: The clinical data of 132 patients diagnosed from February 2016 to October 2019 were collected. Serum miR-499 and SOX6 expressions were detected by RT-qPCR. Optimal cut-off values were determined using receiver operating characteristic curves, based on which patients were divided into low and high miR-499 expression groups, and high and low SOX6 expression groups. Survival curves were plotted using Kaplan-Meier method, and the independent risk factors for MACCE were explored by multivariate logistic regression analysis. A nomogram model was established based on the factors and validated using internal data. Results: AMI group had higher miR-499 expression and lower SOX6 expression than those of control group (P<0.05). After PCI, miR-499 expression decreased and SOX6 expression increased (P<0.05). Low miR-499 expression group had higher 3-year survival and MACCE-free rates than those of high miR-499 expression group (P<0.05). Low SOX6 expression group had lower 3-year survival and MACCE-free rates than those of high SOX6 expression group (P<0.05). AMI history, LVEF, CK-MB, miR-499 and SOX6 expressions were independent risk factors for MACCE (P<0.05). The nomogram model had high accuracy for predicting overall survival, with a concordance index of 0.742 (95%CI=0.684-0.845). Conclusions: AMI patients have increased serum expression of miR-499 and decreased expression of SOX6. High miR-499 expression is an independent risk factor for poor prognosis. The established nomogram model can be used to predict the risk of MACCE after PCI.