Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs.

Gerbens, Frans; Verburg, F.J.; Moerkerk, H.T.B. van; Engel, B.T.; Buist, W.G.; Veerkamp, J.H.; Pas, M.F.W. te

doi:10.2527/2001.792347x

Cited by 152 publications

(101 citation statements)

References 24 publications

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“…Indeed, Alasnier and colleagues in rabbits [12] failed to show any strict association between the relative proportion of oxidative fibers in various muscles and their respective fat content. Genetic variants of FABP have been associated with muscle lipid content in pigs [37], but their involvement at the protein level remains unclear [38]. Finally, in line with our results, LPL activity has been negatively associated to marbling in cattle [39].…”

Section: Discussionsupporting

confidence: 78%

Age-related relationships between muscle fat content and metabolic traits in growing rabbits

Gondret

Hocquette²,

Herpin³

2004

Reprod. Nutr. Dev.

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-This study was aimed at ascribing muscle fat accretion in growing rabbits to changes in several extra-muscular and intra-muscular metabolic pathways. At 10 wk or 20 wk of age (n = 8 per group), tissue lipid content and metabolic indicators of nutrient anabolic or catabolic pathways were simultaneously assessed in the liver, perirenal fat, the heart and the Longissimus lumborum (LL) muscle, together with plasma concentrations in energy-yielding metabolites. Lipid content significantly increased with age (P ≤ 0.01) in the glycolytic LL muscle (+67%) and the oxidative heart (+30%). In the former muscle, it was statistically correlated (r 2 = 0.68; P < 0.01) to the changes in the orientation of muscle metabolism towards an enhanced lipogenic capacity and a depressed capacity for fatty acid transport and nutrient oxidation, and to indications of lower availability in plasma glucose and triglycerides. In the heart, age-related fat accretion was positively associated (r 2 = 0.48, P < 0.01) to intrinsic metabolic changes towards an enhanced lipogenic capacity, together with a lower availability in plasma glucose. Variables representative of cardiac catabolic capacity tended to be negatively correlated to fat content in the heart (r 2 = 0.15, P = 0.07). In growing rabbits, muscle fat content variation was proven to result from a reciprocal balance between catabolic and anabolic fatty acid fluxes, rather than to be assigned to one specific energy metabolic pathway.

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Section: Discussionsupporting

confidence: 78%

Age-related relationships between muscle fat content and metabolic traits in growing rabbits

Gondret

Hocquette²,

Herpin³

2004

Reprod. Nutr. Dev.

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“…This dependence on the analytical model and the quoted differences in the results obtained in other experiments suggest that the polymorphism responsible for the effect found is not the PCR-RFLP analyzed, but another close polymorphism linked in different phases depending on the population. Moreover, in a recent work Gerbens et al [10] could not explain the variation of IMF in crossbred pigs by differences in mRNA and protein expression levels of H-FABP HaeIII genotypes, although these negative results could be due to limitations of the assays. Although we can conclude that our polymorphism is not the causal mutation of the effect observed, it could have a practical interest as a marker for pig breeding programs aimed at improving meat quality while avoiding a backfat increase.…”

Section: Discussionmentioning

confidence: 91%

Test for positional candidate genes for�body composition on�pig chromosome�6

�vilo¹,

Oliver²,

Noguera

et al. 2002

Genet. Sel. Evol.

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-One QTL affecting backfat thickness (BF), intramuscular fat content (IMF) and eye muscle area (MA) was previously localized on porcine chromosome 6 in an F 2 cross between Iberian and Landrace pigs. This work was done to study the effect of two positional candidate genes on these traits: H-FABP and LEPR genes. The QTL mapping analysis was repeated with a regression method using genotypes for seven microsatellites and two PCR-RFLPs in the H-FABP and LEPR genes. H-FABP and LEPR genes were located at 85.4 and 107 cM respectively, by linkage analysis. The effects of the candidate gene polymorphisms were analyzed in two ways. When an animal model was fitted, both genes showed significant effects on fatness traits, the H-FABP polymorphism showed significant effects on IMF and MA, and the LEPR polymorphism on BF and IMF. But when the candidate gene effect was included in a QTL regression analysis these associations were not observed, suggesting that they must not be the causal mutations responsible for the effects found. Differences in the results of both analyses showed the inadequacy of the animal model approach for the evaluation of positional candidate genes in populations with linkage disequilibrium, when the probabilities of the parental origin of the QTL alleles are not included in the model.

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“…PPARG2 induction is followed by the expression of multiple genes and proteins including FABP4 that are involved in intracellular fatty acid transportation, cell growth and differentiation, cellular signaling, gene transcription, and modulation of enzyme activity (Heuckeroth et al 1987, Amri et al 1991, Zimmerman & Veerkamp 2002. The FABP with the widest tissue distribution is FABP3 (muscle FABP) that plays a critical role in the uptake and utilization of NEFA, in fuel selection and energy metabolism, and in adult pigs it is genetically linked to intramuscular fat content (Glatz et al 1998, Binas et al 1999, Gerbens et al 2001, Luiken et al 2003. Recent studies have suggested a more wide spread tissue distribution of FABPs (Gardan et al 2007, Li et al 2007) that may be linked to PPAR action (Motojima 2000, Tan et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Influence of birth weight on gene regulators of lipid metabolism and utilization in subcutaneous adipose tissue and skeletal muscle of neonatal pigs

Williams¹,

Marten²,

Wilson³

et al. 2009

Reproduction

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Epidemiological studies suggest that low-birth weight infants show poor neonatal growth and increased susceptibility to metabolic syndrome, in particular, obesity and diabetes. Adipose tissue development is regulated by many genes, including members of the peroxisome proliferator-activated receptor (PPAR) and the fatty acid-binding protein (FABP) families. The aim of this study was to determine the influence of birth weight on key adipose and skeletal muscle tissue regulating genes. Piglets from 11 litters were ranked according to birth weight and 3 from each litter assigned to small, normal, or large-birth weight groups. Tissue samples were collected on day 7 or 14. Plasma metabolite concentrations and the expression of PPARG2, PPARA, FABP3, and FABP4 genes were determined in subcutaneous adipose tissue and skeletal muscle. Adipocyte number and area were determined histologically. Expression of FABP3 and 4 was significantly reduced in small and large, compared with normal, piglets in adipose tissue on day 7 and in skeletal muscle on day 14. On day 7, PPARA and PPARG2 were significantly reduced in adipose tissue from small and large piglets. Adipose tissue from small piglets contained more adipocytes than normal or large piglets. Birth weight had no effect on adipose tissue and skeletal muscle lipid content. Low-birth weight is associated with tissue-specific and time-dependent effects on lipid-regulating genes as well as morphological changes in adipose tissue. It remains to be seen whether these developmental changes alter an individual's susceptibility to metabolic syndrome.

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Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs.

Cited by 152 publications

References 24 publications

Age-related relationships between muscle fat content and metabolic traits in growing rabbits

Age-related relationships between muscle fat content and metabolic traits in growing rabbits

Test for positional candidate genes for�body composition on�pig chromosome�6

Influence of birth weight on gene regulators of lipid metabolism and utilization in subcutaneous adipose tissue and skeletal muscle of neonatal pigs

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