Associations of Cervicovaginal Lactobacilli With High-Risk Human Papillomavirus Infection, Cervical Intraepithelial Neoplasia, and Cancer: A Systematic Review and Meta-Analysis

Wang, Huiyan; Ma, Yan; Li, Ruonan; Chen, Xixi; Wan, Lijuan; Zhang, Weidong

doi:10.1093/infdis/jiz325

Cited by 55 publications

(52 citation statements)

References 46 publications

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“…crispatus was significantly reduced in women with CIN and cervical cancer. These results are consistent with previous studies including meta-analyses and systematic reviews [ 14 , 15 ]. A low abundance of Lactobacilli spp.…”

Section: Discussionsupporting

confidence: 93%

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Changes of vaginal microbiota during cervical carcinogenesis in women with human papillomavirus infection

Yang

Kim

et al. 2020

PLoS ONE

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Objective To evaluate the changes of vaginal microbiota during cervical carcinogenesis in women with high-risk human papillomavirus infection. Materials and methods Vaginal microbiota was analyzed using next-generation sequencing in women with normal, cervical intraepithelial neoplasia (CIN), or cervical cancer. Results A marked decrease of Lactobacillus crispatus was found in the CIN/cancer groups compared with that in the normal group. The diversity of microorganisms increased in patients with CIN or cervical cancer with HPV infection. Atopobium vaginae (OR 4.33, 95% CI 1.15–16.32), Dialister invisus (OR 4.89, 95% CI 1.20–19.94), Finegoldia magna (OR 6.00, 95% CI 1.08–33.27), Gardnerella vaginalis (OR 7.43, 95% CI 1.78–31.04), Prevotella buccalis (OR 11.00, 95% CI 2.00–60.57), and Prevotella timonensis (OR 6.00, 95% CI 1.46–24.69) were significantly associated with the risk of CIN 2/3 or cervical cancer. Conclusion Women with the CIN and cervical cancer showed a high diversity in vaginal microbiota. Depletion of Lactobacillus crispatus and increased abundance of anaerobic bacteria were detected in women with cervical disease.

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Section: Discussionsupporting

confidence: 93%

“…crispatus , but not L . iners , was associated with the decreased detection of CIN (OR = 0.50, 95% CI = 0.29–0.88) [ 15 ]. A relatively low bacterial diversity and high abundance of Lactobacillus species has been observed in women with a stable vaginal environment [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Changes of vaginal microbiota during cervical carcinogenesis in women with human papillomavirus infection

Yang

Kim

et al. 2020

PLoS ONE

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“…Caucasian and Asian women display a significantly greater prevalence of Lactobacillus in the vagina compared to Hispanic and Black women [8,10,31]. To our knowledge, data with regard to the vaginal bacterial composition of Chinese populations are inadequate [8,18,32]. The analysis, which is performed in a large cohort of women living in a different country and with supposed different hygiene habits [10], is helpful to reinforce the underlying associations.…”

Section: Introductionmentioning

confidence: 99%

“…The analysis, which is performed in a large cohort of women living in a different country and with supposed different hygiene habits [10], is helpful to reinforce the underlying associations. Furthermore, there are few studies about the association between VM and HPV infection and related CIN diseases in Chinese cohorts using high throughput sequencing method [8,18,32].…”

Section: Introductionmentioning

confidence: 99%

Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort

et al. 2020

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Background: In this study, the association between human papillomavirus (HPV) infection and related cervical intraepithelial neoplasia (CIN) or cervical cancer and vaginal microbiome was evaluated in Chinese cohorts. Methods: The vaginal bacterial composition of five groups, HPV-infected women without CINs (HPV, n = 78), women with low-grade squamous intraepithelial lesions (LSIL, n = 51), women with high-grade squamous intraepithelial lesions (HSIL, n = 23), women with invasive cervical cancer (Cancer, n = 9) and healthy women without HPV infection (Normal, n = 68), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. Results: HPV infection increased vaginal bacterial richness and diversity regardless of the status of CINs. The vaginal bacterial richness and diversity were further augmented in women with cervical cancer. Lactobacillus was the most abundant genus in all groups. HPV infection had a negative influence on the abundances of Lactobacillus, Gardnerella and Atopobium. Accordingly, HPV infection increased the relative abundance of Prevotella, Bacillus, Anaerococcus, Sneathia, Megasphaera, Streptococcus and Anaerococcus. The increased proportions of Bacillus, Anaerococcus and the reduced abundance of Gradnerella vaginalis were probably related with the progression of CINs severity. HPV infection without CINs or cancerous lesions was strongly associated with Megasphaera. The most abundant bacterium in the LSIL group was Prevotella amnii. However, Prevotella timonensis, Shuttleworthia and Streptococcaceae at the family level were three taxa related to HSIL. Furthermore, more taxa were associated with the Cancer group including Bacillus, Sneathia, Acidovorax, Oceanobacillus profundus, Fusobacterium, Veillonellaceae at the family level, Anaerococcus and Porphyromonas uenonis. Samples in the Normal group were mostly assigned to CST III. HPV infection converted the vaginal bacterial community structure from CST III to CST IV. Furthermore, the proportions of CST IV were gradually augmented with the progression of the severity of CINs.

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“…Caucasian and Asian women display a signi cantly greater prevalence of Lactobacillus in the vagina compared to Hispanic and Black women [8,10,31]. To our knowledge, data with regard to the vaginal bacterial composition of Chinese populations are inadequate [8,18,32]. The analysis, which is performed in a large cohort of women living in a different country and with supposed different hygiene habits [10], is helpful to reinforce the underlying associations.…”

Section: Introductionmentioning

confidence: 99%

Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort

Chen

Qiu

Wang

et al. 2020

Preprint

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Background: In this study, the association between human papillomavirus (HPV) infection and related cervical intraepithelial neoplasia (CIN) or cervical cancer and vaginal microbiome was evaluated in Chinese cohorts. Methods: The vaginal bacterial composition of five groups, HPV-infected women without CINs (HPV, n=78), women with low-grade squamous intraepithelial lesions (LSIL, n=51), women with high-grade squamous intraepithelial lesions (HSIL, n=23), women with invasive cervical cancer (Cancer, n=9) and healthy women without HPV infection (Normal, n=68), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. Results: HPV infection increased vaginal bacterial richness and diversity regardless of the status of CINs. The vaginal bacterial richness and diversity were further augmented in women with cervical cancer. Lactobacillus was the most abundant genus in all groups. HPV infection had a negative influence on the abundances of Lactobacillus, Gardnerella and Atopobium. Accordingly, HPV infection increased the relative abundance of Prevotella, Bacillus, Anaerococcus, Sneathia, Megasphaera, Streptococcus and Anaerococcus. The increased proportions of Bacillus, Anaerococcus and the reduced abundance of Gradnerella vaginalis were probably related with the progression of CINs severity. HPV infection without CINs or cancerous lesions was strongly associated with Megasphaera. The most abundant bacterium in the LSIL group was Prevotella amnii. However, Prevotella timonensis, Shuttleworthia and Streptococcaceae at the family level were three taxa related to HSIL. Furthermore, more taxa were associated with the Cancer group including Bacillus, Sneathia, Acidovorax, Oceanobacillus profundus, Fusobacterium, Veillonellaceae at the family level, Anaerococcus and Porphyromonas uenonis. Samples in the Normal group were mostly assigned to CST III. HPV infection converted the vaginal bacterial community structure from CST III to CST IV. Furthermore, the proportions of CST IV were gradually augmented with the progression of the severity of CINs.Conclusions: This work interpreted the differential vaginal bacteria under HPV infection and various precancerous or cancerous lesions in a Chinese cohort. We distinguished the specific microbes and the vaginal bacterial structure that were related with the progression of CINs severity in Chinese women.

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Associations of Cervicovaginal Lactobacilli With High-Risk Human Papillomavirus Infection, Cervical Intraepithelial Neoplasia, and Cancer: A Systematic Review and Meta-Analysis

Cited by 55 publications

References 46 publications

Changes of vaginal microbiota during cervical carcinogenesis in women with human papillomavirus infection

Changes of vaginal microbiota during cervical carcinogenesis in women with human papillomavirus infection

Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort

Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort

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