Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia

Abstract

Background. There is strong evidence to suggest that the negative influence of triglyceride-rich lipoproteins (TRLs) on atherosclerosis development and progression is at least partially mediated by their proinflammatory effects. However, the effect of hypertriglyceridemia (HTG) on the subpopulation composition of circulating neutrophils has not been studied so far. The aim of this study was to examine correlations between the level of triglycerides (TGs) and the subpopulation composition of circulating neutrophils in middle-aged patients with dyslipidemia without established atherosclerotic cardiovascular diseases (ASCVDs). Methods. Ninety-one patients with dyslipidemia, including 22 (24.2%) patients with HTG, were enrolled in the study. Phenotying of neutrophil subpopulations was performed through flow cytometry (Navios 6/2, Beckman Coulter, USA). For phenotyping of neutrophil subpopulations, conjugated monoclonal antibodies were used: CD16, PE-Cyanine7 (Invitrogen, USA); CD11b-FITC (Beckman Coulter, USA); CD62L-PE (Beckman Coulter, USA); and CD184 (CXCR4)-PE-CF594 (BD Biosciences, USA). Results. Following the correlation analysis, the TG level directly correlated with the number of circulating leukocytes (r = 0.443; p < 0.0001 ) and neutrophils (r = 0.311; p = 0.008 ). HTG patients displayed a significantly high number of circulating neutrophils with CD16hiCD11bhiCD62Lhi and CD16hiCD11bloCD62Lbr phenotypes. TG levels directly correlated with the number of circulating neutrophils having CD16hiCD11bhiCD62Lhi and CD16hiCD11bloCD62Lbr phenotypes. Following the linear regression analysis, statistically significant correlations between TG levels and neutrophil subpopulations having CD16hiCD11bloCD62Lbr and CD16hiCD11bbrCD62LloCXCR4hi phenotypes were established. Changes in TG levels could explain up to 19.1% of the variability in the number of studied neutrophil subpopulations. Conclusion. Among middle-aged patients without established ASCVDs, patients with HTG demonstrated a significantly higher overall number of neutrophils and neutrophils having CD16hiCD11bhiCD62Lhi (mature neutrophils) and CD16hiCD11bloCD62Lbr (immunosuppressive neutrophils) than patients with normal TG levels. The TG level was associated with an increase in the number of CD16hiCD11bloCD62Lbr and CD16hiCD11bbrCD62LloCXCR4hi (ageing neutrophils) neutrophils, adjusted for the sex and age of the patients.

Immune-Enhancing Treatment among Acute Necrotizing Pancreatitis Patients with Metabolic Abnormalities: A Post Hoc Analysis of a Randomized Clinical Trial

“…26 Among the components of metabolic syndrome, hyperglycemia and hypertriglyceridemia are the two most common metabolic abnormalities, which have frequently been reported to be associated with worse prognosis in AP. 8,9,27 Furthermore, both hyperglycemia and hypertriglyceridemia have been linked to impaired immune function, 10,12 potentially increasing the incidence of infection. However, in our study, only patients with hyperglycemia responded differently to the Tα1 therapy.…”

“…5,6 Hyperglycemia and hypertriglyceridemia are the most two common metabolic disorders in AP, and both of them may increase the risk of infection in AP. [7][8][9][10] A possible explanation is that hyperglycemia can lead to functional damage to the innate immune system, 11,12 and hypertriglyceridemia was also associated with immunosuppression. 10 Moreover, hyperglycemia is thought to attenuate cytokine production, leukocyte recruitment, antibodies and complement effector, and pathogen recognition, leading to neutrophil, macrophage and natural killer cell dysfunction.…”

“…[7][8][9][10] A possible explanation is that hyperglycemia can lead to functional damage to the innate immune system, 11,12 and hypertriglyceridemia was also associated with immunosuppression. 10 Moreover, hyperglycemia is thought to attenuate cytokine production, leukocyte recruitment, antibodies and complement effector, and pathogen recognition, leading to neutrophil, macrophage and natural killer cell dysfunction. 12 In this regard, immune enhancement may have a role in treating AP patients with metabolic abnormalities.…”

Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease

“…For the phenotyping of neutrophil subpopulations, conjugated monoclonal antibodies were used: CD16, PE-Cyanine7 (Invitrogen, Waltham, MA, USA); CD11b-FITC (Beckman Coulter, California, USA); CD62L-PE (Beckman Coulter, CA, USA); and CD184 (CXCR4)-PE-CF594 (BD Biosciences, NJ, USA). The gating strategy was described previously [ 43 ].…”

Investigation of Lymphocyte Subsets in Peripheral Blood of Patients with Dyslipidemia

“…10 Several studies have suggested that dyslipidemia is related to inflammation and immune disorder, but studies on the relationship between dyslipidemia and lymphocyte subsets are still lacking. 11,12 In order to explore the influence of dyslipidemia on immune function, the absolute level of peripheral blood lymphocyte subsets in patients with dyslipidemia was detected, and the correlation between peripheral blood lymphocyte subsets and hypercholesterolemia and hypertriglyceridemia was analyzed in this study, which aim to provide a new idea for clinical diagnosis, therapy and prevention of dyslipidemia.…”