Associations between endotoxin‐induced metabolic changes and temperament in Brahman bulls^,

Abstract

The influence of temperament on the alteration of metabolic parameters in response to a lipopolysaccharide(LPS) challenge was investigated. Brahman bulls were selected based on temperament score. Bulls (10 months; 211±5kg BW; n = 6, 8 and 7 for Calm, Intermediate and Temperamental groups, respectively) were fitted with indwelling jugular catheters to evaluate peripheral blood concentrations of glucose, blood urea nitrogen (BUN),non-esterified fatty acids (NEFA), insulin, epinephrine and cortisol before and after LPS administration (0.5 μg/kg BW LPS). Feed intake was also recorded. Intermediate bulls consumed more feed than the Temperamental bulls during the challenge (p = 0.046). Pre-LPS glucose (p = 0.401) and BUN (p = 0.222) did not differ among the temperament groups. However, pre-LPS insulin (p = 0.023) was lower, whereas pre-LPS NEFA (p < 0.001),cortisol (p < 0.001) and epinephrine (p < 0.001) were greater in Temperamental than in Calm and Intermediate bulls. Post-LPS glucose was increased in Calm and Intermediate bulls but not in Temperamental bulls(p < 0.001). Insulin concentrations post-LPS were greater in Calm than in Intermediate and Temperamental bulls (p < 0.001). Concentrations of NEFA post-LPS were greater in Temperamental than in Calm and Intermediate bulls (p < 0.001). Serum BUN concentration increased post-LPS, with values being greater in Calm and Intermediate than in Temperamental bulls (p = 0.012). Collectively, these data demonstrate that animal temperament is related to the metabolic responses of Brahman bulls following a provocative endotoxin challenge.Specifically, Temperamental bulls may preferentially utilize an alternate energy source (i.e. NEFA) to a greater degree than do bulls of Calm and Intermediate temperaments. The use of circulating NEFA from lipolysis may reduce the negative metabolic consequences of an immune response by allowing for a prompt answer to increasing energy demands required during immunological challenge, compared with the time required for glycogenolysis and gluconeogenesis.

Effects of continuous and increasing lipopolysaccharide infusion on basal and stimulated metabolism in lactating Holstein cows

“…Not surprisingly, circulating BUN is consistently increased in pig models of endotoxemia (Myers et al, 1997;Bruins et al, 2003). Results from ruminants generally agree (Burdick Sanchez et al, 2014), but are more variable (Waggoner et al, 2009a,b;Zarrin et al, 2014), probably due to the contribution of rumen derived ammonia. Further, changes in BUN may be masked by hypophagia-induced alterations in substrate delivery (i.e., AA) and changes to the rumen microbiome (Jing et al, 2014).…”

Physiological and metabolic responses of gestating Brahman cows to repeated transportation12

“…The Wako Autokit for Glucose (Code-No. 439-90901; Wako Diagnostics, Richmond, VA) was used to determine the glucose concentrations of samples as described by Burdick Sanchez et al (2014). Absorbance was measured at 505 nm with a spectrophotometer.…”

“…The enzymatic HR Series NEFA-HR (2) assay (Wako Diagnostics, Richmond, VA) was modified to fit a 96-well format and used to determine NEFA concentrations as described by Burdick Sanchez et al (2014). The minimum detectable concentration for this assay was 0.0014 mmol/L and the intra-and interassay CV were 7.16% and 11.26%, respectively.…”

The effects of acute lipopolysaccharide challenge on dairy goat liver metabolism assessed with 1HNMR metabonomics

“…LPS-induced liver injury has been used to mimic the acute-phase response and analyse the mechanisms of hepatic injury (Masaki et al, 2004;Iwasaki et al, 2008). While the responses of the innate immune and stress systems following an LPS challenge have been well studied, the alterations in carbohydrate, lipid and protein metabolism in the liver remain largely unknown (Burdick Sanchez et al, 2014).…”