Associations among morphological parameters, clinical factors and euploid blastocyst formation

Bilibio, João Paolo; Lorenzzoni, Pânila Longhi; Oliveira, Brenda Mendes de; Nascimento, Flora Leal; Meireles, Arivaldo José Conceição; Nascimento, Fábio Costa do

doi:10.5935/1518-0557.20210008

Cited by 3 publications

(5 citation statements)

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“…Nevertheless, in this study, patients’ ages in the cycles with 2.1PN zygotes were higher than those without 2.1PN zygotes, and the two patients with mosaic embryos were relatively young (30 and 32 years). Although it has been reported that chromosomal aneuploidy is associated with age, 20 , 21 , 22 , 23 further research is required to gain a more complete understanding of whether the aneuploidy of 2.1PN embryos is associated with patient age.…”

Section: Discussionmentioning

confidence: 99%

Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

Takahashi

Hirata

Otsuki

et al. 2022

Reprod Medicine & Biology

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Purpose This study aimed to analyze whether tripronuclear (3PN) zygotes, with two normal‐sized PNs and an additional smaller PN (2.1PN), can be used for embryo transfer. Method(s) A retrospective embryo cohort study was conducted on 695 patients who underwent intracytoplasmic sperm injection treatment. Blastocyst formation rates were compared between 2.1PN and 2PN zygotes and PGT‐A analysis was performed on 15 blastocysts derived from 2.1PN zygotes. Result(s) Blastocyst formation rates were comparable between 2.1PN (43.8%) and 2PN zygotes (54.8%; p = 0.212). The rates of blastocysts with good morphology derived from 2.1 PN and 2PN zygotes were 18.8% and 25.5%, respectively. No significant differences were detected ( p = 0.383). All of the analyzed blastocysts were diploid; however, 13 of these were found to be aneuploid, with a further two being mosaic. Conclusion Our results suggest that 2.1PN embryos can reach blastocyst stage. These blastocysts were diploid, however, predominantly aneuploid, and therefore could not be used for embryo transfer.

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Section: Discussionmentioning

confidence: 99%

Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

Takahashi

Hirata

Otsuki

et al. 2022

Reprod Medicine & Biology

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“…Normal sperm motility was positively correlated with the FR, IR and CPR (Braga et al, 2011). Regarding morphology, a significant decrease in the rates of CP and LB for every 1% decrease in the rate of normal spermatozoa morphology was found (Vural et al, 2005), another reported significantly reduced FR in oligoastenoteratoazoospermia (OAT) (Mazzilli et al, 2017) and more recently a low percentage of spermatozoa with normal morphology was associated to an increased risk of embryonic aneuploidy (Bilibio et al, 2021) and lower FR and CPR (Moubasher et al, 2021). The IR has also been found to be affected by leucocytospermia (Vural et al, 2005), and abnormal spermatozoa morphology and the presence of large or multiple sperm vacuoles were shown to negatively influence the FR, IR and CPR (Braga et al, 2011).…”

Section: The Predictive Value Of Conventional Sperm Parametersmentioning

confidence: 99%

“…Such suggests that women 45 years and beyond do not benefit from IVF/ICSI using their own oocytes (Cetinkaya et al, 2013). Along with the decrease in CPR and LBR, various other outcomes were shown to be associated with the elevation of female age such as higher miscarriage rate (Tan et al, 2014), lower proportion of cycles reaching embryo transfer, decreased number of oocytes retrieved (Tan et al, 2014;Yilmaz et al, 2017;Mahesan et al, 2018), increased risk of embryo aneuploidy (Bilibio et al, 2021), downward trend in fertilisation rate (FR) (Tan et al, 2014), and multiple pregnancy incidence (Tan et al, 2014;Mahesan et al, 2018). In addition, older women had significantly longer stimulation and lower number of normally fertilized (2 pronuclei, 2PN and 2 polar bodies, 2PB) zygotes (2PN zygotes) (Yilmaz et al, 2017;Mahesan et al, 2018).…”

Section: Female Agementioning

confidence: 99%

“…The number of antral follicles, despite not characterizing oocyte quality, represents a good estimator of the primordial follicle pool and, consequently, the quantitative aspect of ovarian reserve (Brugo Olmedo et al, 2013). More recently, AFC of less than 5 was found to be associated to an increased risk of embryo aneuploidy (Bilibio et al, 2021). Yet, AFC is not associated to pregnancy (Cohen et al, 2017) or LB (Busnelli et al, 2014), indicating that…”

Section: Ovarian Reservementioning

confidence: 99%

“…Most studies found no association between male age and FR (Nijs et al, 2011;Abdel Raheem et al, 2013), BP (Beguería et al, 2014;Meijerink et al, 2016), CP (Abdel Raheem et al, 2013;Beguería et al, 2014;Meijerink et al, 2016;Wu et al, 2016;Ma et al, 2018), ongoing pregnancy (Beguería et al, 2014;Meijerink et al, 2016;Wu et al, 2016;Bartolacci et al, 2018), LBR (Abdel Raheem et al, 2013;Beguería et al, 2014;Ma et al, 2018;Mariappen et al, 2018;Park et al, 2018), low neonatal birth weight (<2500g) in singletons (Ma et al, 2018), and preterm birth and birth defects (Wu et al, 2016). However, some studies found that paternal age was an independent factor negatively affecting the FR (Bartolacci et al, 2018), CPR (Yu et al, 2019), IR (Yu et al, 2019), embryonic aneuploidy risk (Bilibio et al, 2021), LBR (McPherson et al, 2018;Yu et al, 2019;Wen et al, 2021) and term-birth rates (liveborn infants between 37 and 41 weeks gestation) (McPherson et al, 2018). McPherson et al (2018) described the evidence of negative associations between paternal age and both viable pregnancies and live births, with a 10% decrease in the probability of pregnancy in women who were aged 35 years with a male partner over 40 years, vs. a male partner aged under 30 years.…”

Section: Male Agementioning

confidence: 99%

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In Vitro Fertilisation and Intracytoplasmic Sperm Injection predictive factors: A review of the effect of female age, ovarian reserve, male age, and male factor on IVF/ICSI treatment outcomes

Ribeiro¹,

Sousa²

2022

JBRA

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The development of assisted reproductive technology has allowed offspring in infertile couples, and specifically, allowed infertile men to conceive through Intracytoplasmic Sperm Injection (ICSI). Despite the proven efficacy of In Vitro Fertilisation (IVF) and ICSI, many factors can influence its success. In this review we present an analysis on the effect of Female age, Ovarian Reserve, Male age and Male factor on the outcomes of IVF/ICSI, to determine if and which can be applied to the practical context. A literature search on PubMed, EMBASE and MEDLINE for relevant articles was elaborated until July 2021, leading to the selection of 234 articles based on their titles. After reading through the abstracts, those that evaluated IVF/ ICSI predicting factors were selected. Finally, only those approaching female age, ovarian reserve, male age and male factor were considered in this review. Higher female age and baseline ovarian markers alterations such as lower anti-Müllerian hormone and antral follicular count, and higher basal follicle-stimulating hormone, were associated with poorer outcomes. The predictive value of Male age and Male factor presented varied results across literature. The multifactorial nature of male fertility makes evaluation difficult. Although the first assessment of male infertility is based on sperm concentration, motility and morphology, semen parameters have shown low prognostic value, whilst sperm DNA alterations gain importance. Nevertheless, results remain controversial. While some factors have proven to predict IVF/ICSI success, other need to be further studied to be applied to practical context to allow the best prognosis possible.

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Predictive factors of aneuploidy in infertile patients undergoing IVF: a retrospective analysis in a private IVF practice

Salame,

Dahdouh,

Aljafari

et al. 2024

Middle East Fertil Soc J

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Background PGT-A has become an important part of IVF treatments. Despite its increased use, there are contradicting results on its role in improving reproductive outcomes of ART cycles. Given that aneuploidy is a main limiting factor for IVF success, we aimed to study the predictive factors of aneuploidy in infertile patients undergoing IVF and hence highlight the patients who would benefit the most from genetic testing. Results A retrospective analysis of 1242 blastocysts biopsied in the setting of PGT-A cycles was performed. The euploid group included 703 embryos, while the aneuploid group had 539 embryos. The factors included in the analyses were the couple’s history as well as the embryo characteristics. The primary outcome was the rate of aneuploid embryos per patient’s history as well as per embryo characteristics. The aneuploidy rate (AR) in our cohort was 43.4%. The woman’s age was found to be a significant predictor (OR 1.045, 95% CI 1.008–1.084, p = 0.016). Biopsy on day 5 as well as degree of expansion 3 was also found to affect significantly (OR 0.724, 95% CI .541–.970, p = 0.03 and OR 2.645, 95% CI 1.252–5.585, p = 0.011). Lack of consanguinity decreased the AR by an OR 0.274 with 95% CI .137–.547, p < 0.001. The number of blastocysts available, trophectoderm quality, embryo grade, gonadotropins as well as trigger used were not found to be significant predictors (p = 0.495, 0.649, 0.264, 0.717 and 0.659 respectively). Conclusion Advanced female age, consanguinity, the day of embryo biopsy, and the degree of blastocyst expansion were all found to affect the incidence of AR. The age of the male partner, cause of infertility, and grade of embryo at biopsy were not found to correlate with aneuploidy.

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Associations among morphological parameters, clinical factors and euploid blastocyst formation

Cited by 3 publications

References 34 publications

Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

In Vitro Fertilisation and Intracytoplasmic Sperm Injection predictive factors: A review of the effect of female age, ovarian reserve, male age, and male factor on IVF/ICSI treatment outcomes

Predictive factors of aneuploidy in infertile patients undergoing IVF: a retrospective analysis in a private IVF practice

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