Association temperature governs structure and apparent thermodynamics of DNA–gold nanoparticles

Beermann, Bernd; Carrillo-Nava, Ernesto; Scheffer, Andy; Büscher, Wolfgang; Jawalekar, Anup M.; Seela, Frank; Hinz, Hans‐Jürgen

doi:10.1016/j.bpc.2006.05.020

Cited by 15 publications

(10 citation statements)

References 11 publications

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“…Conventional ICP-MS is usually used in analyzing the concentrations and compositions of the elements. However, single-particle mode ICP-MS (sp-ICP-MS) is able to perform multiple information analysis on the structure, shape, particle size, and agglomeration of nanoparticles, which extends the application of ICP-MS technology in bioassay [161,162,163,164,165,166,167,168,169,170]. For the first time, Allabashi et al evaluated the possibility of direct determination of AuNPs in colloid solutions by ICP-MS, without previous digestion/dissolution.…”

Section: Icp-ms Biosensormentioning

confidence: 99%

Applications of Gold Nanoparticles in Non-Optical Biosensors

et al. 2018

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Due to their unique properties, such as good biocompatibility, excellent conductivity, effective catalysis, high density, and high surface-to-volume ratio, gold nanoparticles (AuNPs) are widely used in the field of bioassay. Mainly, AuNPs used in optical biosensors have been described in some reviews. In this review, we highlight recent advances in AuNP-based non-optical bioassays, including piezoelectric biosensor, electrochemical biosensor, and inductively coupled plasma mass spectrometry (ICP-MS) bio-detection. Some representative examples are presented to illustrate the effect of AuNPs in non-optical bioassay and the mechanisms of AuNPs in improving detection performances are described. Finally, the review summarizes the future prospects of AuNPs in non-optical biosensors.

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Section: Icp-ms Biosensormentioning

confidence: 99%

Applications of Gold Nanoparticles in Non-Optical Biosensors

et al. 2018

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“…It should be noted that our measurements of T m for DNA-modified vesicles represent temperatures at which almost all DNA is unbound. Although T m for soluble DNA is defined as the temperature at which half the DNA is bound and half unbound, melting profiles of DNA anchored to surfaces and colloids are known to be sharp (28)(29)(30)(31) and therefore we expect T m (vesicles) z T m (DNA) in our experiments.…”

Section: Comparison Of Soluble Dna and Membrane-anchored Dnamentioning

confidence: 99%

“…Melting temperatures of DNA-linked nanoparticle assemblies cannot be understood directly from models of the thermodynamics of single DNA strands in solution (28)(29)(30)(31). Many physical factors may contribute to these differences: i), the entropy cost of tethering one end of the ssDNA to a particle, ii), the entropy loss of the particles when bound, iii), locally increased concentration and orientational restriction of the surface-anchored DNA, and iv), the influence of interparticle interactions on the stability of DNA binding.…”

Section: Introductionmentioning

confidence: 99%

DNA as Membrane-Bound Ligand-Receptor Pairs: Duplex Stability Is Tuned by Intermembrane Forces

Beales

Vanderlick

2009

Biophysical Journal

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We use membrane-anchored DNA as model adhesion receptors between lipid vesicles. By studying the thermal stability of DNA duplex formation, which tethers the vesicles into superstructures, we show that the melting temperature of a 10-base DNA sequence is dependent on the lipid composition of the tethered vesicles. We propose a simple model that describes how the intermembrane interactions tilt the free energy landscape for DNA binding. From our model, we estimate the area per DNA in the binding sites between vesicles and also the total area of the adhesion plaques. We find that vesicles containing a small proportion of cationic lipid that are modified with membrane-anchored DNA can be reversibly tethered by specific DNA interactions and that the DNA also induces a small attraction between these membranes, which stabilizes the DNA duplex. By increasing the equilibrium intermembrane distance on binding, we show that intermembrane interactions become negligible for the binding thermodynamics of the DNA and hence the thermal stability of vesicle aggregates becomes independent of lipid composition at large enough intervesicle separations. We discuss the implications of our findings with regards to cell adhesion and fusion receptors, and the programmable self-assembly of nano-structured materials by DNA hybridization.

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“…The nanoprobes were oriented in a tail-to-tail arrangement, one probe functionalized via a thiol moiety located at the 5'end of the sequence and other at 3' end, with both sequences being contiguous to each other. This way the hybridization of the nanoprobes with the complementary target resulted in the formation of a polymeric cross-linked network, bringing the AuNPs close enough to induce a color change from red to blue (Beermann et al, 2007;Li et al, 2006;Liandris et al, 2009;Storhoff et al, 2005). The efficacy of such cross-linking assay was evaluated by analyzing sputum specimens.…”

Section: Noble Metal Nanoparticlesmentioning

confidence: 99%