Association study of CD154 polymorphisms and serum CD154 level with systemic lupus erythematous in Chinese population

Abstract

The aim of this study was to investigate the association of three polymorphisms of CD154 with risk of SLE in Chinese population. The study population comprised 770 Chinese individuals, including 350 SLE patients and 420 healthy controls. The gene polymorphism was measured using Snapshot SNP genotyping assays and confirmed by sequencing. Serum CD154 (sCD154) level was measured by ELISA. Compared with control group, sCD154 levels were significantly increased in case group (P < 0.001). The minor C allele of rs1126535 was associated with a significantly increased risk of SLE as compared to the major T allele (P < 0.001). Furthermore, an increased frequency of C-G-A haplotype was also detected in case group which associated with an increased risk of SLE (P = 0.009). Notably, patients carrying rs1126535CT/CC genotypes had a higher sCD154 level compared with that carrying rs1126535TT genotype (P < 0.05). Unfortunately, analyses on the association between rs1126535 and several clinical manifestations of SLE failed to find any significant results. In conclusion, these results indicated that CD154 gene polymorphisms may associate with the risk of SLE and may play regulation role in the expression of sCD154 in SLE patients.

Association between CD40 polymorphisms and systemic lupus erythematosus and correlation between soluble CD40 and CD40 ligand levels in the disease: a meta-analysis

“…32,33 Therefore, 14 studies met the inclusion criteria. [7][8][9][10][11][12][13][14][15][16][17][18][19][20] One of the eligible studies included data on two different groups that were independently treated 7 (Table 1). Of these studies, six studies together comprised 1884 patients with SLE and 2882 controls, which were evaluated for CD40 rs4810485 polymorphism; three studies for CD40 rs1883832 polymorphism with 898 patients and 1248 controls; and two studies for rs3765456 polymorphism with 916 patients and 1285 controls.…”

“…7 Some studies investigating the relationship between CD40 polymorphisms and SLE and between soluble CD40 (sCD40) and soluble CD40L (sCD40L) levels and SLE have found associations with SLE susceptibility. [7][8][9][10][11][12][13][14][15][16][17][18][19][20] Reasons for such disparity may be small sample sizes, low statistical power, and/or clinical heterogeneity. To overcome the limitations of individual studies and resolve inconsistencies, we performed this meta-analysis [21][22][23] to review the systematically available evidence on the associations between CD40 rs4810495, rs1883832, and rs376545 polymorphisms and sCD40/sCD40L levels in patients with SLE and controls.…”