Association study between genetic polymorphisms in folate metabolism and gastric cancer susceptibility in Chinese Han population: A case–control study

Abstract

Background Gastric cancer (GC), the second leading cause of cancer mortality behind lung cancer worldwide, is caused by both genetic and environmental factors. In this study, we evaluated the association between the genetic polymorphisms of methylenetetrahydrofolate reductase ( MTHFR ), methionine synthesis reductase ( MTR ), and methyltransferase reductase ( MTRR ) genes and ischemic stroke risk in Chinese population. Methods A case–control study was conducted including 681 patients with GC and 756 healthy controls. Chi‐squared test/Fisher's exact test and genetic model were used to evaluate associations. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using unconditional logistic regression. Results In the allele model, using the chi‐square test, we found that the rs1532268 in MTRR with a minor allele T was significantly associated with increased risk of GC (OR = 1.24, 95% CI, 1.00–1.53; p = 0.048). In the genetic model analysis, we identified that the single‐nucleotide polymorphism of the rs1801133 in MTHFR could increase the GC risk in the recessive model (OR = 1.31, 95% CI, 1.01–1.70; p = 0.042) and log‐additive model (OR = 1.19, 95% CI, 1.02–1.38; p = 0.025). In MTHFR , a strong linkage of rs2274976 and rs1801133 was detected. The haplotype “GC” in the MTHFR gene was found to prominently increase the risk of GC (OR = 1.26, 95% CI: 1.07–1.47; p = 0.005). Other haplotypes did not display the correlativity. Conclusion This study suggested that MTR and MTHFR polymorphisms may contribute to increase the risk of GC.

Association of polymorphisms in LOC105377871 and CASC16 with breast cancer in the northwest Chinese Han population

“…Peripheral blood (5 mL) from each subject was collected in test tube containing ethylenediamine tetraacetic acid and all blood samples were stored in a refrigerator at −80 C. 14 Then, we extracted DNA from the blood samples using the Gold-Mag nanoparticles method (Gold Mag Co., Ltd, Xian, China) 15,16 and measured the DNA concentration and purity with a NanoDrop 2000C (Thermo Scientific, Waltham, MA, USA). 17 We selected SNPs of LOC105377871 and CASC16 with the minor allele frequencies > 5% in global population from the 1000 Genome Project (http://www.internationalgenome.org).…”

Association study between KCNQ1 and KCNQ1OT1 genetic polymorphisms and gastric cancer susceptibility and survival in a Chinese Han population: a case-control study

“…Gastric cancer (GC), a common malignancy with a 5-year survival rate of <25%, is the second leading cause of cancer mortality worldwide (1). The highest GC incidence rate and mortality rate in both sexes are found in East Asia, with approximately 679,100 new cancer cases and 498,000 cancer deaths reported in 2018 in China (2).…”

MTRR rs1532268 polymorphism and gastric cancer risk: evidence from a meta-analysis

“…The characteristics of the included studies were listed in Table 1 . These five studies included four focused on Asian populations 8 , 11 – 13 and one in a White population. 9 In one study, the numbers of patients with CT and TT genotypes were not given individually.…”

“… 9 Among these polymorphisms, CT/TT or TT genotypes of the single nucleotide polymorphism rs1532268 in the MTRR gene coding region are significantly associated with a poorer overall survival compared with the CC genotype. 10 Several studies have evaluated the relationship between the risk of GC and the rs1532268 polyporphism, 8 , 9 , 11 – 13 but the results have been inconsistent. Thus, we conducted this meta-analysis to assess the effect of the rs1532268 polymorphism on GC susceptibility.…”