Association of vitamin D receptor gene polymorphism (VDR) with vitamin D deficiency, metabolic and inflammatory markers in Egyptian obese women

Abstract

Vitamin D deficiency might contribute to the pathogenesis of metabolic syndrome and could cause immune disturbance. The aim of this study is to analyze the associations between Vitamin D receptor (VDR) gene polymorphism, serum 25-hydroxy vitamin D, metabolic and inflammatory biomarkers in Egyptian obese women. The study included 201 obese women with vitamin D deficiency and 249 obese matched age healthy controls with sufficient vitamin D levels. Their age ranged between 25 and 35 years. Inflammatory biomarkers (interleukin-6 and C-reactive protein) and serum 25(OH) D were measured by enzyme-linked immunosorbent assay. Insulin resistance (IR) was determined by the homeostasis model assessment of insulin resistance (HOMA-IR).Vitamin D receptor (VDR) gene polymorphisms of FokI, ApaI, and TaqI were studied by PCR using the restriction fragment length polymorphism (RFLP) technique. Obese women with vitamin D deficiency had significant higher values of inflammatory and metabolic parameters compared to controls. Multivariable-logistic regression showed associations between 25(OH) D deficiency and metabolic components when comparing cases with controls. Moreover, cases carrying polymorphic alleles showed significant lower levels of serum 25(OH) D and higher HOMA-IR, blood pressure levels and lipid parameters compared to those with the wild type homozygote in obese cases with vitamin D deficiency. Vitamin D deficiency in Egyptian obese women with vitamin D deficiency is associated with abnormal metabolic components and abnormal inflammatory biomarkers. Moreover, VDR polymorphisms play important role in immune and inflammation status.

Vitamin D receptor (VDR) gene FokI, BsmI, ApaI, and TaqI polymorphisms and osteoporosis risk: a meta-analysis

“…Several studies have documented that the onset of osteoporosis is caused by VDR gene polymorphisms [81]. VDR gene polymorphisms are also associated with other diseases like breast cancer [105], diabetes [106], myocardial infarction [107], and metabolic syndrome and inflammation [108].…”

Vitamin D receptor gene (FokI, TaqI, BsmI, and ApaI) polymorphisms in children with urinary tract infection

“…6,[14][15][16][17][18][19][20]30,33,34 Some studies have reported a link between vitamin D status, response to vitamin D supplementation, and VDR gene polymorphisms. [35][36][37][38][39] Studies by Martineau et al, Søborg et al, and Lewis et al on pulmonary tuberculosis patients have shown that administration of high doses of VitD have no effect on sputum conversion time when assessed in relation to Fok1 genotype, 35 while other VDR SNPs appeared to influence the response to VitD supplementation. 36,37 The Karpinski study on low-energy bone fractures has demonstrated that ApaI polymorphism recessive "aa" and TaqI polymorphism dominant "TT" genotypes are associated with higher levels of vitamin D in serum.…”

Association of VDR gene variant (rs1544410) with type 2 diabetes in a Pakistani cohort

“…It is predicted that there will be an alarming increase in the incidence of diabetes from 382 million (8.3%) in 2013 to 592 million (10.1%) in 2035. The previous study has shown a strong correlation between the VDR polymorphisms and T2DM-associated metabolic parameters [12]. To briefly explain, the human VDR gene is located on chromosome 12q13.1.…”