Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails

Abstract

Abstract:The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vasoactive intestinal peptide receptor-1 (VIPR-1) was selected as the candidate gene for identifying traits of egg production. A single nucleotide polymorphism (SNP) detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Two mutations (G373T, A313G) were detected in all the tested quail populations. The associated analysis showed that the SNP genotypes of the VIPR-1 gene were significantly linked with the egg weight of G373T and A313G in 398 quails. The quails with the genotype GG always exhibited the largest egg weight for the two mutations in the H and L lines. Linkage disequilibrium (LD) analysis indicated that G373T and A313G loci showed the weakest LD. Seven main diplotypes from the four main reconstructed haplotypes were observed, indicating a significant association of diplotypes with egg weight. Quails with the h1h2 (GGGT) diplotype always exhibited the smallest egg weight and largest egg number at 20 weeks of age. The overall results suggest that the alterations in quails may be linked with potential major loci or genes affecting reproductive traits.

Research Note: Association of VIPR-1 gene polymorphism with growth traits in meat type Japanese quail (Coturnix Japonica)

“…The Hpy CH4 IV site was significantly deviated from HWE ( P < 0.05) in SV, and was consistent with HWE ( P > 0.05) in FG. Pu et al (2016) reported that the detection of 2 SNPs (G373T and A313G) in the VIPR-1 gene of the 3 quail strains was similar to the results of this study.

Figure 1 Detection of PCR amplification products and digestion products at the Bsr D I and Hpy CH4 IV sites of VIPR-1 gene.

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“…The restriction enzyme system was 10 μL: 1 μL 1× buffer, 7.5 μL PCR product, restriction enzyme 0.5 μL Bsr D I (5 U/μL) or Hpy CH4 IV (10 U/μL), 1 μL ddH 2 O. After centrifugation, the Bsr D I system was digested overnight in an incubator at 37°C, while Hpy CH4 IV system was digested in an incubator at 65°C for 1 h ( Pu et al, 2016 ). The enzyme products were detected by 1% agarose gel electrophoresis.…”

“…Furthermore, Zhou et al (2008) have proved that polymorphism of the VIPR-1 gene has a significant impact on broodiness traits of chicken. Pu et al (2016) purported that the polymorphism of the VIPR-1 gene was significantly associated with egg weight in quail. These studies have shown a close correlation between VIPR-1 gene and animals’ economic traits.…”

Remarkable Correlation of Non-Genetic Factors With Growth Traits in Two Breeds of Sheep

“…Interestingly, the recorded LBW values in the Brown-line were decreased at 9 th to 12 th weeks of the experiment. These reduced LBW values may be related to the high efforts performed by this line to produce a higher number of eggs (23). For this reason, larger amounts of the consumed food were utilized in egg production and maintenance requirements instead (29).…”

Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)

“…These observations may refer to a considerable correlation between IGF2 function and egg production. Likewise, several candidate genes are increasingly being reported to be associated with production traits in quails [ 26 – 28 ]. For this reason, the genetic variations within this critical gene may bring several alterations for productive performance in quail populations.…”