Association of variants of miRNA processing genes with cervical precancerous lesion risk in a southern Chinese population

Abstract

The miRNA processing genes play essential roles in the biosynthesis of mammalian miRNAs, and their genetic variants are involved in the development of various cancers. Our study aimed to determine the potential association between miRNA processing gene polymorphisms and cervical precancerous lesions. Five single nucleotide polymorphisms (SNPs), including Ran-GTP () rs14035, exportin-5 () rs11077, rs3742330, rs13078, and rs784567, were genotyped in a case-control study to estimate risk factors of cervical precancerous lesions. The gene-environment interactions and haplotype association were estimated. We identified a 27% decreased risk of cervical precancerous lesions for individuals with minor G allele in rs3742330 (odds ratio (OR) = 0.73, 95% confidence interval (95% CI) = 0.58-0.92, = 0.009). The AG and AG/GG genotypes in rs3742330 were also found to decrease the risk of cervical precancerous lesions (AG compared with AA: OR = 0.51, 95% CI = 0.35-0.73, <0.001; AG/GG compared with AA: OR = 0.54, 95% CI = 0.39-0.77, = 0.001). The GT haplotype in had a risk effect on cervical precancerous lesions compared with the AT haplotype (OR = 1.36, 95% CI = 1.08-1.73, = 0.010). A two-factor ( rs3742330 and human papillomavirus (HPV) infection) and two three-factor (model 1: rs3742330, passive smoking, and HPV infection; model 2: rs3742330, abortion history, and HPV infection) interaction models for cervical precancerous lesions were identified. In conclusion, the genetic variants in the miRNA processing genes and interactions with certain environmental factors might contribute to the risk of cervical precancerous lesions in southern Chinese women.

The 3’ UTR polymorphisms rs3742330 in DICER1 and rs10719 in DROSHA genes are not associated with primary open-angle and angle-closure glaucoma: As case-control study

“…Plausibly, altered levels of DROSHA and DICER1 (as a result of these polymorphisms) may thus either directly affect enzyme function or indirectly, via differential regulation of miRNA expression profiles, can influence disease pathogenesis as discussed above. Rs3742330 polymorphism has been related to DICER1 mRNA dysregulation, wherein the polymorphic A/G and G/G genotypes harbored lower levels of DICER1 mRNA [ 31 , 69 ]. However, the polymorphisms rs3742330 in DICER1 and rs10719 in DROSHA were not associated with POAG and PACG or its related clinical endophenotypes (e.g., IOP and cup/disc ratio) in our Saudi cohort.…”

Polymorphism rs3742330 in microRNA Biogenesis Gene DICER1 Is Associated with Pseudoexfoliation Glaucoma in Saudi Cohort

“…The underlying potential mechanism(s) linking DICER1 polymorphism rs3742330 to PXG pathogenesis is unknown. Rs3742330 polymorphism has been related to dysregulation of DICER1 mRNA, wherein the polymorphic A/G and G/G genotypes harbored lower levels of DICER1 mRNA [ 31 , 32 ]. Altered DICER1 levels (due to polymorphism) may directly affect enzyme function or can indirectly influence disease pathogenesis via differential regulation of miRNA expression.…”

Analysis of microRNA processing machinery gene (DROSHA, DICER1, RAN, and XPO5) variants association with end‐stage renal disease

“…Our results revealed that carrying one DICER1 rs3742330*G allele conferred protection against developing ESRD. Although up to the authors’ knowledge, there are no similar published reports, and emerging evidence shows that DICER1 rs3742330 variant may alter its biologic functions and play essential roles in the susceptibility/protection for various diseases and cancers 36‐42 …”

“…Although up to the authors' knowledge, there are no similar published reports, and emerging evidence shows that DICER1 rs3742330 variant may alter its biologic functions and play essential roles in the susceptibility/protection for various diseases and cancers. [36][37][38][39][40][41][42] According to 43 the DICER1 rs1057035/rs13078/rs3742330 variants was reported to be associated with increased risk of gestational hypertension, 46 while the A-A haplotype of DICER1 rs13078 and rs3742330 were associated with a protective effect on type 2 diabetes mellitus compared with the A-T haplotype. 47 All these findings can support, in part, the observed association of DICER1 variant with ESRD development in the study population.…”