Association of two single nucleotide polymorphisms in the calpastatin gene with tenderness under varying lengths of meat ageing in two native Spanish cattle breeds

Iguácel, Laura Pilar; Calvo, J.H.; Casasús, I.; Serrano, M.; Ripoll, G.; Sarto, P.; Villalba, D.; Alibés, Mireia Blanco

doi:10.1016/j.livsci.2019.103820

Cited by 4 publications

(8 citation statements)

References 22 publications

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“…Studies in controlled environmental and slaughter conditions sometimes only include a small number of animals [35,36] and/or a small number of genotypes [35,37] due to the financial costs involved in raising and slaughtering animals. Other genomic association studies of beef quality have pooled data collected from different herds subjected to varying production systems [38][39][40], with or without electrical stimulation at varying ageing periods [38]. Large studies are often constrained by the cost to genotyping [41], so that the number of SNPs analyzed was sometimes limited to lower-density SNP chips, such as 7K-150K [42,43], inferred through imputation [40,44], or only a few individual SNPs from causal genes were used [35,[37][38][39]45,46].…”

Section: Introductionmentioning

confidence: 99%

“…Other genomic association studies of beef quality have pooled data collected from different herds subjected to varying production systems [38][39][40], with or without electrical stimulation at varying ageing periods [38]. Large studies are often constrained by the cost to genotyping [41], so that the number of SNPs analyzed was sometimes limited to lower-density SNP chips, such as 7K-150K [42,43], inferred through imputation [40,44], or only a few individual SNPs from causal genes were used [35,[37][38][39]45,46]. Data from Bos indicus using a limited capn1 and cast SNP pool suggested that ageing time (<21 d) may alter genomic associations [45], while it has been suggested that ageing decays genetic associations with tenderness phe-notypes over time [47,48].…”

Section: Introductionmentioning

confidence: 99%

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Sustained Effects of Muscle Calpain System Genotypes on Tenderness Phenotypes of South African Beef Bulls during Ageing up to 20 Days

Basson

Strydom

Marle-Köster

et al. 2022

Animals

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The most important factor that determines beef tenderness is its proteolytic activity, and the balance between calpain-1 protease activity and calpastatin inhibition is especially important, while contributions can also arise from calpain-2 and, possibly, calpain-3. The meat ageing process itself affects these processes. To determine whether genotypes in the calpain–calpastatin system can enhance tenderness through a 20-day ageing period, South African purebred beef bulls (n = 166) were genotyped using the Illumina BovineHD SNP BeadChip through a gene-based association analysis targeting the cast, capn3, capn2 and capn1 genes. The Warner–Bratzler shear force (WBSF) and myofibril fragment length (MFL) of Longissimus thoracis et lumborum (LTL) steaks were evaluated between d 3 and d 20 of ageing, with protease enzyme activity in the first 20 h post-mortem. Although several of the 134 SNPs are associated with tenderness, only seven SNP in the cast, capn2 and capn1 genes sustained genetic associations, additive to the ageing-associated increases in tenderness for at least three of the four ageing periods. While most genomic associations were relatively stable over time, some genotypes within the SNP responded differently to ageing, resulting in altered genomic effects over time. The level of ageing at which genomic associations are performed is an important factor that determines whether SNPs affect tenderness phenotypes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sustained Effects of Muscle Calpain System Genotypes on Tenderness Phenotypes of South African Beef Bulls during Ageing up to 20 Days

Basson

Strydom

Marle-Köster

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…Studies in controlled environmental and slaughter conditions sometimes only include a small number of animals [35,36] and/or a small number of genotypes [35,37] due to the financial costs involved in raising and slaughtering animals. Other genomic association studies of beef quality have pooled data collected from different herds subjected to varying production systems [38][39][40], with or without electrical stimulation at varying aging periods [38]. Large studies are often constrained by the cost to genotyping [41], so that the number of SNP analyzed was sometimes limited to lower density SNP chips, like 7K  150K [42,43], inferred through imputation [40,44] or only a few individual SNP from causal genes were used [35,[37][38][39]45,46].…”

Section: Introductionmentioning

confidence: 99%

“…Other genomic association studies of beef quality have pooled data collected from different herds subjected to varying production systems [38][39][40], with or without electrical stimulation at varying aging periods [38]. Large studies are often constrained by the cost to genotyping [41], so that the number of SNP analyzed was sometimes limited to lower density SNP chips, like 7K  150K [42,43], inferred through imputation [40,44] or only a few individual SNP from causal genes were used [35,[37][38][39]45,46]. Data from Bos indicus using a limited capn1 and cast SNP pool suggested that aging time (21d) may alter genomic associations [45], while it has been suggested that aging decays genetic associations with tenderness phenotypes over time [47,48].…”

Section: Introductionmentioning

confidence: 99%

“…Data from Bos indicus using a limited capn1 and cast SNP pool suggested that aging time (21d) may alter genomic associations [45], while it has been suggested that aging decays genetic associations with tenderness phenotypes over time [47,48]. Few studies have explored the effect of different post-slaughter practices like aging period on the association between genotypes and tenderness phenotypes [39,45,49,50] and many studies used a limited number of single nucleotide polymorphisms (SNP), while no data is available from South African beef cattle.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sustained Effects of Muscle Calpain System Genotypes on Tenderness Phenotypes of South African Beef Bulls during Ageing up to 20 Days

Basson¹,

Strydom²,

Marle-Köster³

et al. 2021

Preprint

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The most important factor that determines beef tenderness is its proteolytic activity and the balance between calpain1 protease activity and calpastatin inhibition is especially important, while contributions could arise from calpain2 and possibly calpain3. These processes are however affected by the meat aging process itself. To determine whether genotypes in the calpaincalpastatin system can enhance tenderness throughout a 20 day aging period, South African purebred beef bulls (n=166) were genotyped using the Illumina BovineHD SNP BeadChip, through genebased association analysis targeting the cast, capn3, capn2 and capn1 genes. The WarnerBratzler shear force (WBSF) and myofibril fragment length (MFL) of Longissimus thoracis et lumborum (LTL) steaks were evaluated between d 3 d 20 of aging, with protease enzyme activity in the first 20 h postmortem. Although several of the 134 SNP associated with tenderness, only seven SNP in the cast, capn2 and capn1 genes sustained genetic associations, additive to agingassociated increases in tenderness for at least three of the four aging periods. While most genomic associations were relatively stable over time, some genotypes within SNP responded differently to aging, resulting in altered genomic effects over time. The level of aging at which genomic associations are performed is an important factor that determines whether SNP affect tenderness phenotypes.

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O polimorfismo do gene CAST interfere no amaciamento da carne de bovinos machos Nelores em diferentes condições sexuais e reatividades

Silva

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Tese apresentada para obtenção do título de Doutora em Ciências. Área de concentração: Ciência Animal e Pastagens Piracicaba 2023 Dados Internacionais de Catalogação na Publicação DIVISÃO DE BIBLIOTECA -DIBD/ESALQ/USP Silva, Ana Cláudia da O polimorfismo do gene CAST interfere no amaciamento da carne de bovinos machos nelores em diferentes condições sexuais e reatividades / Ana Cláudia da Silva. --Piracicaba, 2023. 98 p. Tese (Doutorado) --USP / Escola Superior de Agricultura "Luiz de Queiroz". 1. Calpastatina 2. Castração 3. Proteólise 4. Temperamento I. Título AGRADECIMENTOSPrimeiramente а Deus e a Nossa Senhora Aparecida, que me deram força não somente nestes anos de doutorado, mas em todos os momentos da minha vida .Agradeço aos meus pais Ademar e Rose meus maiores exemplos, obrigada por cada incentivo e orientação, pelas orações em meu favor, pela preocupação para que estivesse sempre andando pelo caminho correto. Obrigada por estarem ao meu lado sempre. Amo vocês.E meu agradecimento muito especial a minha irmã gêmea Ana Paula que é minha companheira de todos os momentos, obrigada por sempre me mostrar que eu sou capaz, te amo.Agradeço ao meu companheiro de vida Alex, que jamais me negou apoio, carinho e incentivo.Obrigado, amor da minha vida por aguentar as crises choro e estresse, te amo.Ao Professor Dr. Eduardo pela sua orientação, paciência e atenção, que dedicou seu tempo para me orientar neste trabalho, além disso, por ter me inspirado para que eu me torne uma profissional melhor a cada dia. Agradeço também pela amizade construída nesses 7 anos de convivência.Á Patricia, por tudo que me ensinou, por sua contribuição no meu crescimento pessoal e profissional, por ser uma inspiração. Sou muito grata pela sua amizade. Vou lembrar sempre das nossas inúmeras conversas e risada nos momentos de trabalho. Conviver com você no LAFA, fez desse período muito mais leve.Ao João Pedro, por toda ajuda durante as analises, além da amizade, as risadas, que você compartilhou comigo nessa etapa tão desafiadora da minha vida, fizeram toda a diferença. Á nossa pequena grande família do LAFA Gabriele, Gabriela e Carol. Ao professorSaulo, pela parceria e acolhimento na FZEA/USP, pela oportunidade, pela atenção e colaboração no projeto. Á Professora Aline, as alunas Julia, Iza e Simara, pela ajuda e acolhimento no Laboratório de Biologia Molecular e Micotoxinas. Aos meus queridos amigos Zé Matheus, Oc, Juuh, Giu e Bruna por tornarem os dias da pós mais leves e divertidos Ao Programa de Pós-Graduação em Ciência Animal e Pastagens e à ESALQ/USP, pela oportunidade de realização do meu mestrado. Á CAPES pela bolsa de doutotado concedida. MUITO OBRIGADA! "Conviva com os sabios e será um deles" Provérbios 13.20

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Association of two single nucleotide polymorphisms in the calpastatin gene with tenderness under varying lengths of meat ageing in two native Spanish cattle breeds

Cited by 4 publications

References 22 publications

Sustained Effects of Muscle Calpain System Genotypes on Tenderness Phenotypes of South African Beef Bulls during Ageing up to 20 Days

Sustained Effects of Muscle Calpain System Genotypes on Tenderness Phenotypes of South African Beef Bulls during Ageing up to 20 Days

Sustained Effects of Muscle Calpain System Genotypes on Tenderness Phenotypes of South African Beef Bulls during Ageing up to 20 Days

O polimorfismo do gene CAST interfere no amaciamento da carne de bovinos machos Nelores em diferentes condições sexuais e reatividades

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