Association of TRIM22 with the type 1 interferon response during primary human cytomegalovirus infection in THP-1 macrophages

Abstract

As a response factor of interferon, tripartite motif (TRIM) 22 was reported to exert antiviral activity against viruses. In this study, THP-1 macrophages were infected with human cytomegalovirus (HCMV) to establish the HCMV lytic infection model. The mRNA levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and interferonbeta (IFN-β) were significantly up-regulated in THP-1 macrophages at different infection time and titers. Moreover, for the first time, upregulation of TRIM22 expression was found during HCMV infection at both mRNA and protein levels in THP-1 macrophages. Furthermore, IFN-β could induce TRIM22 expression in THP-1 macrophages or HCMV infected THP-1 macrophages. Depletion of TRIM22 increased replication activity of HCMV with increasing of HCMV titers and HCMV proteins. In conclusion, it is the first report that HCMV can induce TRIM22 activation through IFN-β signaling and TRIM22 can suppress replication of HCMV in THP-1 macrophages.

Intrauterine high androgen promotes obesity of the offspring of rats with polycystic ovarian syndrome via activating macrophage-angiogenesis-related androgen signaling

“…In adipose tissue, inflammation inhibits adipocyte expansion and differentiation, alters adipocyte endocrine secretion, and induces extracellular matrix remodelling (Khan et al, 2009). Macrophages play an important role in chronic low-grade inflammation (Hong et al, 2020;Li et al, 2019;Lima et al, 2018). During the development of PCOS, it has been found that hyperandrogenic conditions may lead to the recruitment macrophages under the influence of chemokines (Lima et al, 2018).…”