Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins

Mesquita, Alice Leite; Rezende, Cíntia Silva Minafra e; Mesquita, Albenones José de; Jardim, Eurione Antônio Garcia da Veiga; Kipnis, Ana Paula Junqueira

doi:10.1590/s1517-83822012000200034

Cited by 23 publications

(17 citation statements)

References 12 publications

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“…There was a nonsynonymous mutation in exon 3 (SNP 9787C>T) which had changed amino acid tyrosine to lysine. All these SNPs were reported earlier (seven SNPs by Wang et al 9 one SNP by Mesquita et al ) 10 There was no novel SNP found in TLR4 gene in the present study. The SNPs and other variations found in the nucleotide sequences of expressed regions of bovine TLR4 gene among the crossbred populations are presented in Table 1.…”

Section: Nucleotide Sequence Of Bovine Tlr4 Genesupporting

confidence: 72%

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TLR4 Gene Polymorphism and its Association with Mastitis Resistance in Indigenous and Crossbred Cattle of Tamil Nadu, India

Girish¹,

Sn²,

Smk³

et al. 2016

JDVAR

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TLR4 gene is one of the candidate genes for mastitis resistance in cattle. This gene has three exons and PCR with nine sets of primers produced nine amplicons. The amplicons were sequenced from both ends, assembled and screened for SNPs. Totally, 221 dairy cows (Jersey crossbreds, Holstein Friesian crossbreds and indigenous breeds) were genotyped either by PCR-RFLP or tetra-primer ARMS-PCR. Genotyping results showed that one SNP was in 5'UTR region, two SNPs in intron 1, one in exon 2 and seven in exon 3. Out of 11 SNPs, allele frequencies were found to differ between healthy and mastitic cows in six SNPs viz. SNPs 245, 5053, 5134, 9422, 9787 and 9794. At SNP position 5134, the G allele frequency got reduced from 0.71 in healthy cows to 0.50 in mastitic cows but the A allele frequency increased from 0.29 in healthy cows to 0.50 in mastitic cows. The SNP 5053T>G resulted from transversion while the SNPs 5087G>A and 5134G>A resulted from transitions. In SNP 5134G>A, the frequency of both the alleles were in equal proportions in mastitic animals. The SNP 9787C>T in exon 3 had shown no appreciable change in the allele frequencies in both normal and mastitis animals. Homology modeling of TLR4 gene revealed no change in the length of the amino acids of TLR4 protein. The amino acid tyrosine present in the position 674 in the protein chain of Bos taurus was replaced lysine in crossbreds. No association was found between TRL4 genotypes and somatic cell scores in the breeds studied.

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Section: Nucleotide Sequence Of Bovine Tlr4 Genesupporting

confidence: 72%

“…Similarly, no association was found between TLR4 gene SNP 245G>C and SCS in earlier studies also. 5,11 On the contrary, associations were found between SNP 245 and SNP 5087 and SCS 10 and between SNP 9787 and SCS. 9…”

Section: Association Of Tlr4 Gene With Scsmentioning

confidence: 88%

TLR4 Gene Polymorphism and its Association with Mastitis Resistance in Indigenous and Crossbred Cattle of Tamil Nadu, India

Girish¹,

Sn²,

Smk³

et al. 2016

JDVAR

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“…According to Sender et al (2013) the best cumulative genotype does not identify individual genes, and animals are selected based on the estimated cumulative impact of multiple genes on resistance to mastitis. Mesquita et al (2012) studied Brazilian Holsteins for TLR4 polymorphism indicated that animals with combined genotypes AACCCC, GGTCGG, and GACCGC presented the lowest SCS and have the potential to be applied as molecular markers for assisted animal selection to improve milk quality. Six combined genotypes of two TLR4 gene SNPs (c.9421C>T and c.2021C>T) were observed, though only one cow had a CC/TC and CC/TT combined genotype.…”

Section: Discussionmentioning

confidence: 99%

The influence of TLR4 gene polymorphisms on milk quality and composition of Lithuanian Holstein cows

et al. 2020

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This study investigated bovine TRL4 gene c.9421C>T, c.2021C>T and c.-10C>T polymorphisms and their relationship with somatic cell count and indicators of milk composition. Blood samples were collected from 152 Lithuanian Holstein dairy cows. The method of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) was used to genotype TLR4 gene polymorphisms. The data concerning somatic cell count and milk composition indicators (fat, protein, lactose) were analysed. The influence of genes and statistical significance of differences between different genotypes was evaluated by the one-factor dispersion analysis (ANOVA). Results showed that c.9421C>T was significantly associated with somatic cell count in milk. Also a significant association between the genotypes of c.9421C>T and somatic cell count was found (P<0.05). Cattle with the TT genotype showed the lowest somatic cell count. TLR4 gene polymorphisms c.2021C>T and c.-10C>T have no significant effect on mastitis resistance and milk composition. Analysis of the combined genotypes TC/CC of c.2021C>T and c.9421C>T allowed us to determine the possible association of SNPs with the somatic cell count and lactose content. The study showed a significant association between the somatic cell count (SCC) and TLR4 polymorphism c.9421C>T in Lithuanian Holstein cows. SCS of cows with a TT genotype was significantly lower and indicated the association of TT genotype with resistance to mastitis in c.9421C>T and allele T might be the beneficial allele for mastitis resistance.

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“…The remaining variants identified all represented synonymous variations. TLR4 region analyzed for somatic cell score (SCS) and 3 SNPs were associated with high and low SCS values (intron1 rs8193046 A/G, exon3 rs8193060 C/T, 5'UTR rs29017188 C/G) [52]. ACC haplotype was associated with low SCS whereas GTG haplotype was associated with high SCS.…”

Section: Discussionmentioning

confidence: 99%

Determination of Genetic Variations of Toll-like Receptor (TLR) 2, 4 and 6 with Next Generation Sequencing in Native Cattle Breeds of Anatolia and Holstein Friesian

Bilgen¹,

Kul²,

Offord³

et al. 2016

Preprint

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Abstract:In recent years, the focus of disease resistance and susceptibility studies in cattle have been on determining patterns in the innate immune response of key proteins, such as Toll-like receptors (TLR). In the bovine genome, there are 10 TLR family members and, of these, TLR2, TLR4 and TLR6 are specialized in recognition of bacterial ligands. Indigenous cattle breeds of Anatolia have been reported to show fewer signs of clinical bacterial infections, such as bovine tuberculosis and mastitis, and it is hypothesized that this might be due to a less stringent genetic selection during breeding.In contrast, Holstein-Friesian cattle have been under strong selection for milk production, which may have resulted in greater susceptibility to diseases. To test this hypothesis, we have compared the TLR2, TLR4 and TLR6 genes of Anatolian Black (AB), East Anatolian Red (EAR), South Anatolian Red (SAR), Turkish Grey (TG), and Holstein (HOL) cattle using Next Generation Sequencing. The SAR breed had the most variations overall, followed by EAR, AB, TG and HOL. TG had the most variations for TLR2 whereas SAR had the most variations in TLR4 and TLR6. We compared these variants with those associated with disease and susceptibility traits. We used exon variants to construct haplotypes, investigated shared haplotypes within breeds and determined candidate haplotypes for disease resistance phenotype in Anatolian cattle breeds.

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Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins

Cited by 23 publications

References 12 publications

TLR4 Gene Polymorphism and its Association with Mastitis Resistance in Indigenous and Crossbred Cattle of Tamil Nadu, India

TLR4 Gene Polymorphism and its Association with Mastitis Resistance in Indigenous and Crossbred Cattle of Tamil Nadu, India

The influence of TLR4 gene polymorphisms on milk quality and composition of Lithuanian Holstein cows

Determination of Genetic Variations of Toll-like Receptor (TLR) 2, 4 and 6 with Next Generation Sequencing in Native Cattle Breeds of Anatolia and Holstein Friesian

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