Association of the Virus of Lymphocytic Choriomeningitis with Erythrocytes of Infected Animals

Shwartzman, Gregory

doi:10.1128/jb.46.5.482-483.1943

Cited by 5 publications

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References 4 publications

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“…However, blood smears of mouse RV-151 (Table IV) showed definite fluorescent-stainable antigen as fine granules on or in roughly 10 per cent of the erythrocytes. This observation is in agreement with the reported association of LCM virus with erythrocytes (17).…”

Section: That In the Acute Infection Liver Sections Varied In The Amsupporting

confidence: 94%

Immunofluorescent Studies of the Histopathogenesis of Lymphocytic Choriomeningitis Virus Infection

Wilsnack

Rowe

1964

The Journal of Experimental Medicine

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The pathogenesis of lymphocytic choriomeningitis (LCM) virus infection of mice has been subjected to intensive study by virus titration and histopathologic techniques (1-5). There are a number of unresolved questions relating to the pathogenesis of the various types of infection which require study of the distribution of virus at the cellular, rather than at the whole organ level. This report describes a direct immunofluorescent antibody test for LCM antigen, and its application to study of cellular distribution of infection in acutely and congenitally infected mice. Materials and MethodsMice.--Weanling mice of the HA/ICR strain were purchased from Charles River Mouse Farms, Brookline, Massachusetts. Latent LCM infection was not detected in these mice.LCM Virus.--The CA 1371 (mouse brain-passaged) and WCP (viscerotropic) strains of LCM virus, and the procedures for preparation of virus stocks have been described previously (6). Swiss mice congenitally infected with LCM virus were from the colony of carrier mice initiated by Haas (7); these mice were in approximately the 41st generation as carriers.Virus Titrations.--Virus suspensions (clarified by centrifugation at 1800 Rmt for 10 minutes) and serial 10-fold dilutions were made in medium 199 with 3 per cent inactivated calf serum. Materials were held in an ice bath, and inoculations made as soon as possible after preparation of suspensions. Weanling mice were inoculated intracerebrally with 0.03 ml, using 6 mice per dilution. Survivors were rechallenged at 14 days postinoculation with approximately 1000 LD60 of strain CA 1371 LCM virus, and held an additional 14 days. In. fectivity titers, based on typical death or immunity to challenge as the endpoint, were calculated according to the method of Reed and Muench, and are expressed as the logt0 of the number of IDs0 per 0.03 mi of 10 per cent suspension. Because of the high degree of pathogenicity of the CA 1371 strain following intracerebral inoculation, immunity tests were not considered necessary to evaluate infectivity titration endpoints with this strain.Tissue Cultures.--Primary cultures of trypsinized kidney ceils of monkey (rhesus and Cercopitkecus), rabbit, and Syrian hamster were used, as well as WI-26 cells and primary cultures of HA/ICR mouse embryo. All cells were grown on 9 x 35 mm coverslips in Leighton tubes. 829

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Section: That In the Acute Infection Liver Sections Varied In The Amsupporting

confidence: 94%

Immunofluorescent Studies of the Histopathogenesis of Lymphocytic Choriomeningitis Virus Infection

Wilsnack

Rowe

1964

The Journal of Experimental Medicine

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“…A survey program and laboratory screening procedures were therefore developed which had as their object the diagnosis of blood-stream infection with viruses having red cell affinities. In order to economize in time and materials, our survey was directed primarily toward clinical material of the following categories: (1) 3 conditions regardless of thc actual presence of anemia at the time of examination, (4) healthy contacts of patients with viremia, and ( 5 ) healthy persons, including blood donors, and patients suffering from clinical conditions in which no suspicion of viremia existed. As now set up, the complete survey of an individual case includes the following panel of tests:…”

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confidence: 99%