Association of the tumor necrosis factor-alpha −308 G/A polymorphism with nasal polyposis

Abstract

Nasal polyposis (NP) is a chronic inflammatory disease in which several molecular and cellular interactions play important roles. Tumor necrosis factor-alpha (TNF-alpha) is a major pro-inflammatory cytokine with a key role in immune inflammatory responses in NP. Altered levels of TNF-alpha, which may occur due to polymorphisms in the TNF-alpha promoter region, may also be associated with NP susceptibility. Given these facts, we investigated the possible association of the TNF-alpha -308 G/A single nucleotide polymorphism (SNP) with NP. In this study, 97 consecutive adult patients with NP and 95 age- and gender-matched controls were recruited. For identification of SNP, restriction fragment length polymorphism analysis after polymerase chain reaction was carried out. The NP group had a significantly higher rate of polymorphism compared to controls (p = 0.015). Logistic regression analysis revealed that the presence of the TNF-alpha -308 G/A SNP is an independent risk factor for NP development (OR, 3.68; CI, 1.27-10.7; p = 0.016). The presence of a mutation failed to influence disease severity on the basis of resistance to medical and/or surgical treatment. This study suggests a possible linkage of a SNP in the TNF-alpha promoter with NP. These results need to be confirmed with multicentre studies for more precise interpretation and corroborative studies for investigating the influence of polymorphism on transcriptional activity.

The role of tumor necrosis factor-alpha -308 G/A and transforming growth factor-beta 1 -915 G/C polymorphisms in childhood idiopathic thrombocytopenic purpura

“…Polymorphisms of TNF-α promoter are associated with high levels of TNF-α and have been studied as a determinant of susceptibility to numerous diseases [12,[21][22][23][24][25]. A study that examined inflammatory cytokines and Fcγ receptor polymorphisms in chronic childhood ITP reported that polymorphisms in 2 low-affinity FcγR genes (IIIA and IIIB) and 2 proinflammatory cytokine genes (TNF and LTA) were associated with chronic childhood ITP [7].…”

TNF-α and IL-1 β Cytokine Gene Polymorphism in Patients with Nasal Polyposis

“…In the North America region, Randolph et al (21) found that TNF-α gene promoter region allele frequency did not affect asthma formation, whereas Witte et al (22) found the A allele to be a risk factor. The different results in the Turkish population (7,8, and our study) can be explained by genetic variations in different regions of the country, patient selection criteria, and differences in study design. Moreover, the effect of allelic frequencies and genotypes of the TNF-α gene promoter region on the transcription of TNF-α is not clear.…”

“…Single nucleotide polymorphisms (SNPs) in these pro-inflammatory mediators have been studied for genetic susceptibility to NP development, but the literature findings regarding the dominant genotype or allele causing NP formation are conflicting. Some authors claim that the TNF-α GA polymorphism in the -308 promoter region is a risk factor for NP development, whereas others have found that the A allele frequency is a risk factor (7)(8)(9). Some authors have found no relationship between the TNF-α GA polymorphism and NP development (4,10).…”

Evaluation of autoinflammatory disease genes in nasal polyposis*

“…Batikhan et al showed that TNF-a-308G > A single nucleotide polymorphism (SNP) may play a role in the etiology of nasal polyposis (10).…”