Association of the rs555172 polymorphism in SENCR long non-coding RNA and atherosclerotic coronary artery disease

Abstract

Introduction: Variants in long non-coding RNAs (lncRNAs) have been implicated as potential biomarkers in prediction of complex disorders such as coronary artery disease (CAD). Studies considering the impact of the SENCR antisense lncRNAs on CAD have not established yet in Iranian population. This study aimed to investigate the association between SENCR rs555172 polymorphism and CAD in south Iranian population. Methods: Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was performed to determine the allele and the genotype distribution of SENCR lncRNA polymorphism in 150 patients with CAD compared with 149 healthy controls through this hospital-based case-control study. Results: The frequency of AA, AG, and GG genotypes in cases were 32.7%, 44.7%, and 22.6%, and in controls were 26.8%, 49%, and 24.2%, respectively. Association was not found with any of the genotypes in comparison of cases and controls. The allelic frequencies did not differ between cases and controls. Cross-tabulating the population based on the gender, the frequency of the GG genotype was significantly higher among women of the case group compared to men. The difference was not seen in the control group between two sexes. Conclusion: The results suggested that the SENCR gene polymorphism did not confer susceptibility to CAD.

Association of polymorphisms in MALAT1 with risk of coronary atherosclerotic heart disease in a Chinese population

“…Gao et al reported that the risk rs2067051A allele in lncRNA- H19 was more frequent in CAD patients than in controls [ 20 ]. Shahmoradi et al reported that the frequency of rs555172 GG genotype in lncRNA9 was significantly higher among female CAD patients compared to male patients [ 24 ]. In this study, we found a reduced risk of rs619586 AG/GG genotypes in MALAT1 with CAD.…”

“…Previous studies have shown that single nucleotide polymorphisms (SNPs) in lncRNA contribute to the risk of CAD [ 18 – 24 ]. We therefore hypothesized that SNPs in MALAT1 may be related to the individuals’ susceptibility to CAD.…”

Definition and review on a category of long non-coding RNA: Atherosclerosis-associated circulating lncRNA (ASCLncRNA)

“…By using amplification refractory mutation system-polymerase chain reaction, SENCR can be detected stably and sensitively in blood samples of patients with CAD (Shahmoradi et al, 2017). Besides, a large-scale investigation focused on the effect of pioglitazone on type 2 diabetes found that the expression of SENCR in the blood is related to the evaluation of diastolic function after drug treatment, and that its indication in drug treatment effect is better than other existing indicators (De Gonzalo-Calvo et al, 2016b).…”

Peer Review #1 of "Definition and review on a category of long non-coding RNA: Atherosclerosis-associated circulating lncRNA (ASCLncRNA) (v0.1)"

“…By using amplification refractory mutation system-polymerase chain reaction, SENCR can be detected stably and sensitively in blood samples of patients with CAD (Shahmoradi et al 2017). Besides, a large-scale investigation focused on the effect of pioglitazone on type 2 diabetes found that the expression of SENCR in the blood is related to the evaluation of diastolic function after drug treatment, and that its indication in drug treatment effect is better than other existing indicators (de Gonzalo-Calvo et al 2016b).…”