Association of the microbiota dysbiosis in the hepatopancreas of farmed crayfish (Procambarus clarkii) with disease outbreaks

Wu, Zhenbing; Zhang, Qianqian; Zhang, Tanglin; Chen, Jiwang; Wang, Shuyi; Hao, Jingwen; Lin, Yun; Li, Aihua

doi:10.1016/j.aquaculture.2021.736492

Cited by 24 publications

(20 citation statements)

References 81 publications

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“…Many studies have shown the potential effects of Lactococcus on the growth and digestive enzyme activities of shrimp ( Adel et al, 2017 ). In addition, Lactococcus belonging to Firmicutes participates in the degradation of undigested carbohydrates and amino acids into short-chain fatty acids, promoting the metabolic function of crayfish ( Lopetuso et al, 2013 ; Wu et al, 2021 ) and thus playing an important role throughout the growth stages of P. clarkii .…”

Section: Discussionmentioning

confidence: 99%

“…P. clarkii is omnivorous, feeding mainly on zooplankton during the juvenile period and on large aquatic plants during the adult period ( Li, 2006 ), which may be related to the large increase of Dysgonomonas at the adult stage. Citrobacter is a typical conditional pathogen widely found in nature, and its infection in P. clarkii activates the natural immune response of P. clarkii ( Liu et al, 2020 ); however, in general, Citrobacter is normally dominant in the intestine of crayfish ( Wu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Xie

Zhang²,

et al. 2021

Front. Microbiol.

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This study aimed to determine the effect of the growth stage of Procambarus clarkii on their intestinal microbiota. Intestinal samples of five different growth stages of P. clarkii (first instar, second instar, third instar, juvenile, and adult) from laboratory culture were analyzed through the Illumina MiSeq high-throughput sequencing platform to determine the intestinal microbiome of crayfish. The alpha diversity decreased along with the growth of the crayfish, with the relative abundance of the microbiota changing among stages; crayfish at closer development stages had a more comparable intestinal microbiota composition. A comparative analysis by principal component analysis and principal coordinate analysis showed that there were significant differences in the intestinal microbiota of crayfish among the different growth stages, except for the first two stages of larval crayfish, and the intestinal microbiota showed a consistent progression pattern from the larval stage to the juvenile stage. Some microbiota showed stage specificity, which might be the characteristic microbiota of different stages of growth. According to FAPROTAX functional clustering analysis, the three stages of larvae were clustered together, while the juvenile and adult stages were clustered separately according to the growth stage, indicating that, in the early stages of larval development, the function of the intestinal flora was similar; as the body grew and developed, the composition and function of the intestinal microbiota also changed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Xie

Zhang²,

et al. 2021

Front. Microbiol.

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“…Also, the hepatopancreas is an important indicator of crustaceans’ host health condition ( Deng et al, 2017 ). Previous studies of the crayfish’s ( Procambarus clarkia ) hepatopancreatic microbiota revealed that diseased organisms underwent dysbiosis in the gut-hepatopancreatic microbiota, affecting lipid synthesis pathways ( Wu et al, 2021 ). Although, the relationship between gut dysbiosis and physiological effects in lipid synthesis remains unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The dysbiosis of the intestine can contribute to several shrimp diseases, such as acute hepatopancreatic necrosis disease (AHPND) and white feces syndrome (WFS) ( Hossain, Dai & Qiu, 2021 ; Huang et al, 2020a ). Recently, Wu et al (2021) revealed that dysbiosis in the hepatopancreatic microbiota of the crayfish ( Procambarus clarkii ) is associated with disease outbreaks.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal variations in the gastrointestinal microbiome of the white shrimp, Litopenaeus vannamei

Garibay-Valdez

Cicala

Martínez‐Porchas

et al. 2021

PeerJ

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The shrimp gut is a long digestive structure that includes the Foregut (stomach), Midgut (hepatopancreas) and Hindgut (intestine). Each component has different structural, immunity and digestion roles. Given these three gut digestive tract components’ significance, we examined the bacterial compositions of the Foregut, Hindgut, and Midgut digestive fractions. Those bacterial communities’ structures were evaluated by sequencing the V3 hypervariable region of the 16S rRNA gene, while the functions were predicted by PICRUSt2 bioinformatics workflow. Also, to avoid contamination with environmental bacteria, shrimp were maintained under strictly controlled conditions. The pairwise differential abundance analysis revealed differences among digestive tract fractions. The families Rhodobacteraceae and Rubritalaceae registered higher abundances in the Foregut fraction, while in the Midgut, the families with a higher proportion were Aeromonadaceae, Beijerinckiaceae and Propionibacteriaceae. Finally, the Cellulomonadaceae family resulted in a higher proportion in the Hindgut. Regarding the predicted functions, amino acid and carbohydrate metabolism pathways were the primary functions registered for Foregut microbiota; conversely, pathways associated with the metabolism of lipids, terpenoids and polyketides, were detected in the Midgut fraction. In the Hindgut, pathways like the metabolism of cofactors and vitamins along with energy metabolism were enriched. Structural changes were followed by significant alterations in functional capabilities, suggesting that each fraction’s bacteria communities may carry out specific metabolic functions. Results indicate that white shrimp’s gut microbiota is widely related to the fraction analyzed across the digestive tract. Overall, our results suggest a role for the dominant bacteria in each digestive tract fraction, contributing with a novel insight into the bacterial community.

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“…Cray sh are freshwater crustaceans that have become increasingly popular in epigenetic, microbiome, stem cell and evolutionary biology research. 14,19,102,103,110,114,37,40,42,82,87,94,100,101 This is primarily due to Procambarus spp small size, high fecundity, and overall environmental and nutritional adaptability. 40,78,82,101 Additionally, there is clear potential for cray sh to become a mainstay neuroregenerative and immunologic animal model.…”

Section: Introductionmentioning

confidence: 99%

Housing, Husbandry, and Clinical Techniques for Crayfish (Procambarus clarkii) used in Biomedical Research

Palillo¹,

Palillo²,

Glon³

et al. 2021

Preprint

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Crayfish (Decapoda: Astacoidea & Parastacoidea), are amongst the few animals that have stem cells within hemolymph, with the capacity to continuously produce differentiated neuronal structures throughout life. As crayfish and other invertebrates continue to become common models in research to study human disease, it is vital that we develop universal laboratory standards and guidelines on housing and husbandry practices. This publication presents introductory data on housing, husbandry, hemolymph collection and statistically supported anesthesia trials, to support future research endeavors. Evaluation of housing, husbandry, clinical and anesthetic techniques in Procambarus clarkii maintained in a biomedical research setting were performed. An option for hemolymph collection, in the area termed, the Ventral Coelomic Hemolymph Collection Zone (VCHCZ) is presented as a technique to assess hemocytes. Additionally, Wright Giemsa stained slides of crayfish hemolymph were evaluated by a board-certified veterinary clinical pathologist for interpretation and confirmation of hemolymph collection. The housing and husbandry experiments were performed over a duration of 37 days. Mortality rates and physical health assessments were performed. The following water quality parameters were concurrently evaluated: temperature, light cycle, pH, KH, GH, conductivity, total dissolved solids, salinity, ammonia, nitrite, and nitrate. Anesthetic techniques were evaluated between four experimental groups: (A) immersion in buffered MS-222 (50 mg/L), (B) immersion in buffered MS-222 (150 mg/L) (C) immersion in Propofol (65 mg/L) and (D) Propofol injection (100 mg/kg) into VCHCZ. Housing and husbandry techniques were validated with 0% mortality and normal species-specific behaviors were observed. MS-222 immersion had no observable effect on crayfish. Propofol immersion (65 mg/L) created sedative effects allowing for appropriate handling. Propofol injection (100 mg/kg) into VCHCZ successfully created a deep anesthetic plane that would allow for more invasive or surgical procedures, without adverse effects during or after recovery.

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Association of the microbiota dysbiosis in the hepatopancreas of farmed crayfish (Procambarus clarkii) with disease outbreaks

Cited by 24 publications

References 81 publications

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Longitudinal variations in the gastrointestinal microbiome of the white shrimp, Litopenaeus vannamei

Housing, Husbandry, and Clinical Techniques for Crayfish (Procambarus clarkii) used in Biomedical Research

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