Association of the <i>CASP3</i> rs4647602 Gene Polymorphism with Periodontitis in South Indians of Tamil Ethnicity

Loka, Arya Mitra; Ponnaiyan, Deepa; Parthasarathy, Harinath; Tadepalli, Anupama; Victor, Dhayanand John

doi:10.1089/gtmb.2022.0018

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…VEGF-A, which belongs to the vascular endothelial growth factors (VEGFs), plays an important role in the pathological process of periodontitis [52]. Caspase-3, which mainly regulates cell apoptosis, is also involved in the pathogenesis of periodontal disease [53]. All the above-mentioned genes have been con rmed by other researchers to participate in the development of periodontitis, which further validates the accuracy and reliability of our results.…”

Section: Discussionsupporting

confidence: 81%

Investigating the Mechanism of Astragalus in the Treatment of Periodontitis through Bioinformatics Analysis

li,

feng,

Jang

et al. 2024

Preprint

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Background: Periodontitis, a common oral inflammatory disease which may cause premature tooth loss, was proved can be treated by Astragalus, but the detailed mechanisms are still not clear. We validated and discussed the molecular mechanism by using bioinformatics methods and cell experimental, and in order to clarify the mechanism of Astragalus during the treatment of periodontitis. Methods:The active ingredients of Astragalus and their corresponding targets were obtained using the TCMSP database, and the periodontitis-related targets were obtained from DrugBank database, GeneCards database etc., then GO and KEGG analyses were performed based on Metascape database. Astragalus active ingredients and related targets network, Astragalus-active components-targets of periodontitis network, and Astragalus-active components- periodontitis targets-signaling pathways network were constructed by using Cytoscape3.9.0 software. Thereafter, Molecular docking and molecular dynamic simulation were analyzed in Discovery Studio 2019 software and Gromacs 2021.2 software package respectively, in order to evaluate the stability of combination between active components and core targets. Results:17 compounds of Astragalus and 464 corresponding targets were obtained and 5 major active ingredients were screened from the drug active ingredients- periodontitis gene network. PPI network analysis revealed the top 10 core potential targets, 7 of them have suitable crystal structure and can be used for molecular docking, including interleukin-6 (IL6), tumor necrosis factor (TNF), RAC-α serine/threonine protein kinase (AKT1), interleukin-1β(IL1β), prostaglandin G/H synthase-2 (PTGS2), matrix metalloproteinase-9 (MMP9), and Caspase3 (CASP3). In addition, 58 GO terms and 146 KEGG pathways were identified. 5 major active ingredients and 7 core targets which mentioned above were docked molecularly in Discovery Studio 2019 software. Molecular dynamics simulations confirmed that there has a stable combination between Caspase3 and Kaempferol ligand system. Conclusions: Based on the results of network pharmacology, molecular docking and molecular dynamics, it can be concluded that Astragalus has multiple active ingredients, and targets different signaling pathways to regulate the inflammatory response, immune response and oxidative stress in order to play a beneficial role in the treatment of periodontitis, especially Kaempferol can combine with Caspase3 stably to inhibit the cell apoptosis, our data provide solid evidences and enlightenment for the clinical application of Astragalus in future.

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Section: Discussionsupporting

confidence: 81%

Investigating the Mechanism of Astragalus in the Treatment of Periodontitis through Bioinformatics Analysis

li,

feng,

Jang

et al. 2024

Preprint

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“…Genetic Polymorphism. Genetic polymorphisms, which are characterized by an exaggerated phenotypic expression of a protein due to alteration in the genetic makeup, such as in periodontitis, that have gained much of an attention as they play a role in the immunomodulation [33]. Various promising genetic alterations can be mapped to the occurrence of both periodontitis and migraine apart from being associated with common risk factors such as the proinflammatory burden, smoking, and stress [34].…”

Section: Neurovascular Mediatorsmentioning

confidence: 99%

Exploring the Potential Consortium of Migraine and Periodontitis

Ponnaiyan,

Rughwani,

Shetty

et al. 2024

International Journal of Dentistry

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Objectives. Various researches have shown periodontitis to share common pathophysiological pathways with systemic diseases such as diabetes, cardiovascular diseases, and osteoporosis and recently neurological disorders. This article provides a narrative review summarizing the various linking mechanisms and the nature of association between two multifactorial diseases—periodontitis and migraine. Materials and Methods. A literature search was performed for articles related to periodontitis and migraine up till the year 2023 which yielded totally 14 articles. There were only three randomized controlled clinical trials; therefore, we were unable to conduct a systematic review and focused on a narrative review. The keywords searched were “migraine”, “periodontitis” and “biomarkers” in PubMed/Medline, Web of Science, and Embase databases. Any article related to the association of periodontitis and migraine and the dental management of subjects with headache disorders were included and studies with migraine and other dental diseases were excluded. Results. It is found that the occurrence of periodontitis and migraine are associated with each other. There is reasonable evidence to believe that periodontitis and migraine are linked by direct and indirect mechanisms which can eventually lead to chronic inflammatory conditions like periodontitis worsening neurovascular conditions such as migraine. However, upon detailed analysis it was found that the strength of association is weak owing to the presences of various common confounding and risk factors. Conclusions. The association between periodontitis and migraine cannot be denied, however, not all the criteria are fulfilled while examining the nature of association and future long-term studies are required to prove the same. Clinical Relevance. Various studies have reported poor periodontal health in patients with migraine. The risk of exacerbation of migraine also increases in subject undergoing dental therapy if the triggering factors are manipulated. Hence, knowing the precise pathophysiologic mechanisms linking both the diseases would be favorable in planning treatment protocols for subjects with migraine.

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“…Signal transduction activities, including chemotaxis and the activation of inflammatory and bone cells, are triggered when specific receptors like CC chemokine receptor (CCR) 2 and CCR4 interpret chemokine signals. The severity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is correlated with increased expression of the protein it encodes [11].…”

Section: Introductionmentioning

confidence: 99%

Genetic Association of the C-C Motif Chemokine Ligand 2 (CCL2) rs1024611 Polymorphism With Periodontitis

Ahmed Khan,

Murthykumar,

Ganapathy

2023

Cureus

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IntroductionTo a large extent, a person's susceptibility to developing periodontitis is determined by their genetic makeup. Research has shown that chemokines generated during an immune response can harm the periodontal ligaments, gingiva, and alveolar bone. Various chemokine genes located on different chromosomes contribute to periodontitis, and one such gene is C-C motif chemokine ligand 2 (CCL2), associated with the rs1024611 polymorphism, which is part of a cytokine gene cluster on the q-arm of chromosome 17. ObjectiveOur specific objective was to investigate whether CCL2 polymorphisms could influence the relative risk of developing periodontitis. Building on these findings, we aimed to compare the frequency of a specific single nucleotide polymorphism (SNP) in the CCL2 gene between individuals with and without periodontitis. Materials and methodsFifty participants were enrolled in the study after obtaining informed consent and ethical clearance. Clinical assessments, including probing pocket depth, clinical attachment loss, and bleeding on probing, were utilized to classify individuals into two groups: a control group (Group A, n=25) and a periodontitis group (Group B, n=25). DNA extraction from collected samples involved drawing 2 ml of venous blood from the antecubital fossa and transferring it into a sterile tube with a pinch of ethylene diamine tetraacetic acid (EDTA) to prevent clotting. DNA extraction was performed and polymorphisms of CCL2 were assessed through polymerase chain reaction (PCR) and restriction enzyme digestion. ResultsThe periodontitis group consisted of 25 patients, with an average age of 39.0±0.22 years, who met the American Academy of Periodontology's 2018 criteria for at least stage II periodontitis. The control group comprised 25 individuals with an average age of 41.3±0.49 years. Regarding the CCL2 gene polymorphism (rs1024611), there was no substantial variation in genotype frequencies between the patients and controls (p = 0.695). An agarose gel electrophoretogram, along with a standard DNA ladder, demonstrated partial amplification of the CCL2 gene spanning the polymorphism site (rs1024611). Genotypes observed were as follows: homozygous AA -333 bp; heterozygous AG -333 + 250 + 73 bp; homozygous GG -250 + 73 bp. ConclusionIn conclusion, there is no significant association between the CCL2 gene polymorphism rs1024611 and susceptibility to periodontitis.

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Association of the CASP3 rs4647602 Gene Polymorphism with Periodontitis in South Indians of Tamil Ethnicity

Cited by 3 publications

References 30 publications

Investigating the Mechanism of Astragalus in the Treatment of Periodontitis through Bioinformatics Analysis

Investigating the Mechanism of Astragalus in the Treatment of Periodontitis through Bioinformatics Analysis

Exploring the Potential Consortium of Migraine and Periodontitis

Genetic Association of the C-C Motif Chemokine Ligand 2 (CCL2) rs1024611 Polymorphism With Periodontitis

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