Association of the hypha‐related protein Pra1 and zinc transporter Zrt1 with biofilm formation by the pathogenic yeast <i>Candida albicans</i>

Kurakado, Sanae; Arai, Rika; Sugita, Takashi

doi:10.1111/1348-0421.12596

Cited by 15 publications

(17 citation statements)

References 24 publications

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“…Interestingly, previously published results on zinc effects on Candida biofilm formation are contradictory. For example, Kurakado et al demonstrated an increase in C. albicans biofilm formation as a response to ZnSO 4 and a decrease in response to zinc chelator 81 while several others report Zn-associated biofilm inhibition [82][83][84] . These contradictions could be partly due to different average (Fig.…”

Section: Discussionmentioning

confidence: 99%

Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

Rosenberg

Visnapuu

Vija

et al. 2020

Sci Rep

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Spread of pathogenic microbes and antibiotic-resistant bacteria in health-care settings and public spaces is a serious public health challenge. Materials that prevent solid surface colonization or impede touch-transfer of viable microbes could provide means to decrease pathogen transfer from high-touch surfaces in critical applications. ZnO and Ag nanoparticles have shown great potential in antimicrobial applications. Less is known about nano-enabled surfaces. Here we demonstrate that surfaces coated with nano-ZnO or nano-ZnO/Ag composites are not cytotoxic to human keratinocytes and possess species-selective medium-dependent antibiofilm activity against Escherichia coli, Staphylococcus aureus and Candida albicans. Colonization of nano-ZnO and nano-ZnO/Ag surfaces by E. coli and S. aureus was decreased in static oligotrophic conditions (no planktonic growth). Moderate to no effect was observed for bacterial biofilms in growth medium (supporting exponential growth). Inversely, nano-ZnO surfaces enhanced biofilm formation by C. albicans in oligotrophic conditions. However, enhanced C. albicans biofilm formation on nano-ZnO surfaces was effectively counteracted by the addition of Ag. Possible selective enhancement of biofilm formation by the yeast C. albicans on Zn-enabled surfaces should be taken into account in antimicrobial surface development. Our results also indicated the importance of the use of application-appropriate test conditions and exposure medium in antimicrobial surface testing. Biofilms are by far the preferred lifestyle of bacteria 1 , mostly in diverse nutrient-limited environmental niches. Biofilm communities cause biomass buildup on solid surfaces that results in major expenses in marine traffic, water systems maintenance and in the industrial sector. Biofilms can also harbor potential human pathogens in food industry, health-care facilities, drinking water systems and on high-touch surfaces in public spaces. It is estimated that hard to treat pathogenic biofilms account for over 80% of all human microbial infections 2 with antibiotic-resistant ESKAPE pathogens 3 including six nosocomial pathogens commonly associated with multidrug resistance and virulence (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp.) being the most problematic in the field. Biofilms pose a major medical challenge as they can be over 1,000-fold more tolerant to antibiotics than their planktonic counterparts 4. Spatially heterogeneous natural selection in biofilm milieu also contributes to antibiotic resistance development and transfer 5,6 as well as producing antibiotic-resistant bacteria that are more fit and not easily outcompeted in the absence of the drug 7. Controversially, most of the methods used to assess antimicrobial properties of surface materials, irrespective of their proposed application, either use planktonic cultures or study indirect effects such as release of antimicrobial compounds from the surfaces 8. Such methods might...

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Section: Discussionmentioning

confidence: 99%

Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

Rosenberg

Visnapuu

Vija

et al. 2020

Sci Rep

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“…In C. albicans, zinc is important in terms of both biofilm development and maintenance. The expression of genes related to zinc acquisition increases in biofilm cells of C. albicans [14,18]. Our previous report suggested that C. albicans biofilm formation is regulated by zinc and that TPEN inhibits biofilm formation [14].…”

Section: Discussionmentioning

confidence: 92%

“…The expression of genes related to zinc acquisition increases in biofilm cells of C. albicans [14,18]. Our previous report suggested that C. albicans biofilm formation is regulated by zinc and that TPEN inhibits biofilm formation [14]. Depletion of divalent cations, such as iron, calcium, and magnesium, inhibit biofilm formation by Aspergillus fumigatus and Cryptococcus neoformans [19,20].…”

Section: Discussionmentioning

confidence: 96%

“…We previously found that the expression of Pra1 and Zrt1 genes involved in zinc utilization in C. albicans was greater in biofilms than in planktonic cells [14]. The pra1 and zrt1 gene-deficient strains exhibited decreased abilities to form biofilms and also hyphae, which are essential for biofilm formation [14]. Moreover, zinc addition caused increased biofilm formation, and zinc depletion by a chelator inhibited biofilm formation in C. albicans [14].…”

Section: Introductionmentioning

confidence: 99%

“…The pra1 and zrt1 gene-deficient strains exhibited decreased abilities to form biofilms and also hyphae, which are essential for biofilm formation [14]. Moreover, zinc addition caused increased biofilm formation, and zinc depletion by a chelator inhibited biofilm formation in C. albicans [14]. These findings suggest that zinc is a key metal for biofilm formation in C. albicans.…”

Section: Introductionmentioning

confidence: 99%

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N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii

et al. 2020

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Objective: Trichosporon asahii is the major causative fungus of disseminated or deep-seated trichosporonosis and forms a biofilm on medical devices. Biofilm formation leads to antifungal drug resistance, so biofilm-related infections are relatively difficult to treat and infected devices often require surgical removal. Therefore, prevention of biofilm formation is important in clinical settings. In this study, to identify metal cations that affect biofilm formation, we evaluated the effects of cation chelators on biofilm formation in T. asahii. Results: We evaluated the effect of cation chelators on biofilm formation, since microorganisms must assimilate essential nutrients from their hosts to form and maintain biofilms. The inhibition by N,N,N′,N′-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) was greater than those by other cation chelators, such as deferoxamine, triethylenetetramine, and ethylenediaminetetraacetic acid. The inhibitory effect of TPEN was suppressed by the addition of zinc. TPEN also inhibited T. asahii hyphal formation, which is related to biofilm formation, and the inhibition was suppressed by the addition of zinc. These results suggest that zinc is essential for biofilm formation and hyphal formation. Thus, zinc chelators have the potential to be developed into a new treatment for biofilm-related infection caused by T. asahii.

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Clinical Significance, Molecular Formation, and Natural Antibiofilm Agents of Candida albicans

Abdulghani,

Zore

2024

Advances in Antifungal Drug Development

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Association of the hypha‐related protein Pra1 and zinc transporter Zrt1 with biofilm formation by the pathogenic yeast Candida albicans

Cited by 15 publications

References 24 publications

Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii

Clinical Significance, Molecular Formation, and Natural Antibiofilm Agents of Candida albicans

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