Association of the HLA‐G 14‐bp insertion/deletion polymorphism with juvenile idiopathic arthritis and rheumatoid arthritis

Abstract

We tested the possible association of the 14-bp polymorphism of the HLA-G gene in the course of two inflammatory diseases, rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA). Patients and controls were genotyped for the 14-bp polymorphism by polymerase chain reaction with specific primers for the exon 8 of the human leukocyte antigen (HLA)-G gene and the amplified fragment was visualized in a 6% polyacrylamide gel. A total of 106 JIA patients, 265 RA patients, 356 healthy adults and 85 healthy children were genotyped for the 14-bp polymorphism. Female JIA patients presented a higher frequency of the -14 bp allele when compared with female healthy children (0.743 and 0.500, corrected P=0.003), which reflected in the JIA group as a whole. This increased frequency of the -14-bp allele was observed in all JIA subtypes. In RA patients, no differences in allelic and genotypic frequencies were observed between patients and controls. No correlations were observed among genotype and disease severity or clinical manifestations. Our data suggest that the HLA-G -14 bp allele is probably a risk factor for JIA, mainly in females. Considering the differences observed in relation to gender, we suggest that hormonal differences can interfere with the development of JIA. Considering the RA patients, our data agree with results from the literature and highlight the differences in the etiology of RA and JIA.

Relationship between the HLA-G 14bp insertion/deletion polymorphism and susceptibility to autoimmune disease: a meta-analysis

“…Both studies showed consistent results: there was no difference between genotype and allelic frequencies for this polymorphism. A single study for each of the associations between this polymorphism and JIR, multiple sclerosis, ulcerative colitis, Crohn's disease, idiopathic dilated cardiomyopathy, pemphigus vulgaris, and non-segmental vitiligo has been reported (Gazit et al, 2004;Glas et al, 2007;Kroner et al, 2007;Lin et al, 2007;Veit et al, 2008;Jeong et al, 2014). Thus, we did not perform AID subtype analyses of these cases, and the results should be interpreted cautiously.…”

“…Many studies have investigated the complications of pregnancy, such as recurrent spontaneous abortion and pre-eclampsia. Several studies have suggested that the HLA-G 14-bp INS/DEL polymorphism is associated with AIDs, such as SLE (Rizzo et al, 2008;Veit et al, 2009;Wu et al, 2009;Consiglio et al, 2011), RA Veit et al, 2008), juvenile idiopathic arthritis (Veit et al, 2008), multiple sclerosis (Kroner et al, 2007), ulcerative colitis (Glas et al, 2007), Crohn's disease (Glas et al, 2007), idiopathic dilated cardiomyopathy (Lin et al, 2007), pemphigus vulgaris, and non-segmental vitiligo (Jeong et al, 2014).…”

“…In the current meta-analysis, we found no association between this polymorphism and SLE. Rizzo et al (2009) and Veit et al (2008) studied the association between the 14-bp INS/DEL polymorphism and RA. Both studies showed consistent results: there was no difference between genotype and allelic frequencies for this polymorphism.…”

Population genetic structure of HLA-G gene in North-west Region of Russian Federation

“…Для идентификации полиморфизма 3741*ins/del 14 п. н. использованы последовательности праймеров, приведенные в работе Veit et al, 2008.…”

Association between two polymorphisms in the HLA-G gene and angiographic coronary artery disease

“…This polymorphism is linked with another polymorphism (rs1632933) of HLA-G. Previous studies (27)(28)(29) have provided evidence of a significant involvement of these polymorphisms in situations where immune dysregulation plays a crucial pathogenetic role, including several inflammatory-mediated human disorders.…”