Association of the Growth-Arrest-Specific Protein Gas7 with F-Actin Induces Reorganization of Microfilaments and Promotes Membrane Outgrowth

She, Bin-Ru; Liou, Gunn‐Guang; Lin‐Chao, Sue

doi:10.1006/excr.2001.5435

Cited by 62 publications

(69 citation statements)

References 37 publications

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“…Gas7 has been found to interact physically with cytoskeletons including actin and tubulin (She et al, 2002;Uchida et al, 2009). For example, in vitro and in vivo studies indicated that Gas7, especially the C-terminal FCH domain, is able to form complexes with F-actin, resulting in extended actin bundles (She et al, 2002). Gas7 also potentiates formation of tau-associated microtubulin sheets, suggesting a possible role in enhancing microtubule polymerization and linking this protein with Alzheimer's disease Akiyama et al, 2009).…”

Section: Discussionmentioning

confidence: 97%

“…Insofar as these Gas7 antibody-reactive large proteins are above 72 kDa in size, it is not clear whether they represent Gas7 proteins. It has been shown that Gas7 binds to actin (She et al, 2002) and phospho-Tau, thereby enhancing microtubule polymerization Uchida et al, 2009). The equal loading of atubulin and GAPDH for the tested samples appears to indicate that Gas7b is substantially suppressed by the shRNA used.…”

Section: Knockdown Of Gas7b Reduces Cisplatin-induced Apoptosis In Humentioning

confidence: 97%

“…Gas7 is predominantly expressed in brain tissues, including in the cerebral cortex, hippocampus, and cerebellum (Ju et al, 1998). The subcellular level Gas7 is distributed mainly in the cytoplasm, with preferential localization in submembrane regions (Ju et al, 1998;She et al, 2002).…”

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confidence: 99%

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Knockdown of growth‐arrest‐specific gene 7b (gas7b) using short‐hairpin RNA desensitizes neuroblastoma cells to cisplatin: Implications for preventing apoptosis of neurons

Hung

Chao

2010

J of Neuroscience Research

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Efficient control of cell survival and cell proliferation is critical for the development of neuron cells. Earlier, we observed that growth arrest-specific gene 7 (Gas7) plays a role in controlling neuritogenesis in mammals. In the present study, we report that the Gas7b isoform is involved in controlling growth arrest and apoptosis of neuroblastoma cells in response to various stimuli. Accordingly, knockdown of Gas7b using small-hairpin RNA (shRNA) was shown to reduce apoptosis induced either by serum starvation or by the antineoplastic agents cisplatin and nocodazole in human neuroblastoma SH-SY5Y cells. Gas7b knockdown also enhanced the ability of the treated cells to form clones in response to cisplatin. On the other hand, forced expression of Gas7a or Gas7b isoform in mouse neuroblastoma Neuro2A cells, which express a defective Gas7 gene, rendered the cells proapoptotic and vulnerable to cisplatin-induced apoptosis. In addition, Neuro2A cells that overexpressed Gas7 showed a reduced ability to form clones. Overexpression of Gas7 produced similar but less extensive effects in nonneuronal HEK293 cells. Taken together, our observations suggest that Gas7b is involved not only in neuritogenesis but also in the regulation of neuronal cell death.

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Section: Discussionmentioning

confidence: 97%

Section: Knockdown Of Gas7b Reduces Cisplatin-induced Apoptosis In Humentioning

confidence: 97%

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Knockdown of growth‐arrest‐specific gene 7b (gas7b) using short‐hairpin RNA desensitizes neuroblastoma cells to cisplatin: Implications for preventing apoptosis of neurons

Hung

Chao

2010

J of Neuroscience Research

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“…Several members of the BAR domain super family provide a link between the membrane and the membrane-associated cytoskeleton and play essential roles in fundamental biological processes such as endocytosis, exocytosis, and cell motility (16,17). Gas7b binds actin via its F-BAR domain to enhance actin polymerization (18). Recently, we demonstrated that excess Gas7b protein directly binds to microtubules and inhibits the motility of kinesin (19).…”

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confidence: 99%

Gas7b (Growth Arrest Specific Protein 7b) Regulates Neuronal Cell Morphology by Enhancing Microtubule and Actin Filament Assembly

Gotoh

Hidaka

Hirose

et al. 2013

Journal of Biological Chemistry

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Background: Growth arrest specific protein 7b (Gas7b) protein levels are significantly reduced in the brain tissue of patients with Alzheimer disease. Results: Gas7b enhances microtubule bundling and microtubule cross-linking with the actin filament. Conclusion: Gas7b regulates neural cell morphology by altering the cytoskeletal organization. Significance: A decrease in Gas7b levels impairs neural cell function.

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“…Expression of the Gas protein family is strictly related to the growth arrest state of mammalian cells 158,159 (see Figure 4 Box E) and may be involved in various cellular activities including microfilaments reorganization, cell cycle, DNA synthesis, and apoptosis 158,160,161 . Smith and Steitz 162 showed that upon reduction of the mTOR activity, Gas5 transcript levels increased.…”

Section: Cell Growth Regulationmentioning

confidence: 99%

Scale-down of CHO cell fed-batch cultures

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Association of the Growth-Arrest-Specific Protein Gas7 with F-Actin Induces Reorganization of Microfilaments and Promotes Membrane Outgrowth

Cited by 62 publications

References 37 publications

Knockdown of growth‐arrest‐specific gene 7b (gas7b) using short‐hairpin RNA desensitizes neuroblastoma cells to cisplatin: Implications for preventing apoptosis of neurons

Knockdown of growth‐arrest‐specific gene 7b (gas7b) using short‐hairpin RNA desensitizes neuroblastoma cells to cisplatin: Implications for preventing apoptosis of neurons

Gas7b (Growth Arrest Specific Protein 7b) Regulates Neuronal Cell Morphology by Enhancing Microtubule and Actin Filament Assembly

Scale-down of CHO cell fed-batch cultures

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