Association of the expression of an SR-cyclophilin with myeloid cell differentiation

Giardina, Steven L.; Coffman, J.Daniel; Young, Howard A.; Potter, Stephen J.; Frey, Joyce L.; Ortaldo, John R.; Anderson, Stephen

doi:10.1182/blood.v87.6.2269.bloodjournal8762269

Cited by 7 publications

(3 citation statements)

References 17 publications

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“…CeCyp8 and CeCyp9, along with human NK‐Cyp, were also reported in this study to possess this ‘moca’ domain, but in all cases the proteins show no linkage between species, with only the two C. elegans cyclophilins appearing to have a recent, and in their case distinct, common ancestor (Figure 1A). hNK‐Cyp functions almost solely on the outer cell membrane of natural killer cells (Alkhatib et al , 1997; Anderson et al , 1993; Giardina et al , 1996) as an important component in the recognition of infected cells (Chambers et al , 1994), a process not found in the other compared organisms. The evolution and conservation of this ‘moca’ domain is therefore elusive when looked at in the context of this dendrogram (Figure 1A) and the function of the proteins that are known to possess it.…”

Section: Resultsmentioning

confidence: 99%

Identification and comparative analysis of the peptidyl‐prolyl cis/trans isomerase repertoires of H. sapiens, D. melanogaster, C. elegans, S. cerevisiae and Sz. pombe

Pemberton

Kay

2005

Comp Funct Genom

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The peptidyl-prolyl cis/trans isomerase (PPIase) class of proteins comprises three member families that are found throughout nature and are present in all the major compartments of the cell. Their numbers appear to be linked to the number of genes in their respective genomes, although we have found the human repertoire to be smaller than expected due to a reduced cyclophilin repertoire. We show here that whilst the members of the cyclophilin family (which are predominantly found in the nucleus and cytoplasm) and the parvulin family (which are predominantly nuclear) are largely conserved between different repertoires, the FKBPs (which are predominantly found in the cytoplasm and endoplasmic reticulum) are not. It therefore appears that the cyclophilins and parvulins have evolved to perform conserved functions, while the FKBPs have evolved to fill ever-changing niches within the constantly evolving organisms. Many orthologous subgroups within the different PPIase families appear to have evolved from a distinct common ancestor, whereas others, such as the mitochondrial cyclophilins, appear to have evolved independently of one another. We have also identified a novel parvulin within Drosophila melanogaster that is unique to the fruit fly, indicating a recent evolutionary emergence. Interestingly, the fission yeast repertoire, which contains no unique cyclophilins and parvulins, shares no PPIases solely with the budding yeast but it does share a majority with the higher eukaryotes in this study, unlike the budding yeast. It therefore appears that, in comparison with Schizosaccharomyces pombe, Saccharomyces cerevisiae is a poor representation of the higher eukaryotes for the study of PPIases.

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Section: Resultsmentioning

confidence: 99%

Identification and comparative analysis of the peptidyl‐prolyl cis/trans isomerase repertoires of H. sapiens, D. melanogaster, C. elegans, S. cerevisiae and Sz. pombe

Pemberton

Kay

2005

Comp Funct Genom

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“…three SR domains. NK-TR1 has PPIase, protein folding and chaperone activities and is important for myeloid cell differentiation and cytotoxic function of natural killer cells(51,52). Similar sequence motifs are present in Bmcyp from Brugia malayi, a filarial nematode…”

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confidence: 95%

A serine/arginine-rich nuclear matrix cyclophilin interacts with the C- terminal domain of RNA polymerase II

Bourquin

Štagljar²,

Meier³

et al. 1997

Nucleic Acids Research

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The largest subunit of RNA polymerase II shows a striking difference in the degree of phosphorylation, depending on its functional state: initiating and elongating polymerases are unphosphorylated and highly phosphorylated respectively. Phosphorylation mostly occurs at the C-terminal domain (CTD), which consists of a repetitive heptapeptide structure. Using the yeast two-hybrid system, we have selected for mammalian proteins that interact with the phosphorylated CTD of mammalian RNA polymerase II. A prominent isolate, designated SRcyp/CASP10, specifically interacts with the CTD not only in vivo but also in vitro . It contains a serine/arginine-rich (SR) domain, similar to that found in the SR protein family of pre-mRNA splicing factors, which is required for interaction with the CTD. Most remarkably, the N-terminal region of SRcyp includes a peptidyl-prolyl cis - trans isomerase domain characteristic of immunophilins/cyclophilins (Cyp), a protein family implicated in protein folding, assembly and transport. SRcyp is a nuclear protein with a characteristic distribution in large irregularly shaped nuclear speckles and co-localizes perfectly with the SR domain-containing splicing factor SC35. Recent independent investigations have provided complementary data, such as an association of the phosphorylated form of RNA polymerase II with the nuclear speckles, impaired splicing in a CTD deletion background and inhibition of in vitro splicing by CTD peptides. Taken together, these data indicate that factors directly or indirectly involved in splicing are associated with the elongating RNA polymerases, from where they might translocate to the nascent transcripts to ensure efficient splicing, concomitant with transcription.

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“…Its expression is increased by IL2 activation of the cells [ 16 ]. Interference with normal NKTR expression in HL-60 cells markedly suppresses many differentiation-associated events [ 17 ]. Overexpression of the centrosome-associated serine/threonine kinase Aurora Kinase A (AURKA) has been demonstrated in advanced prostate cancer and high-grade prostatic intraepithelial neoplasia lesions.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis

Bai

Zhong

et al. 2022

Aging

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Objective: Liver metastasis is one of the prognostic factors of colorectal cancer (CRC). The aim of this study is to identify biomarkers that facilitate easier detection of liver metastasis. Methods: Significance Analysis of Microarrays (SAM) and Array Data Analyzer (ADA) were applied used for the analysis of differentially differently expressed mRNAs. mRNA expression was verified by quantitative real-timer reverse transcriptiontase polymerase chain reaction (qRT-PCR). Immunohistochemistry were was used to show natural killer-tumor recognition (NKTR) expression in CRC. NKTR-knockdown CRC cells were constructed obtained by using short hairpin RNA (shRNA). Followed by CCK-8 assay, plate colony formation test, and transwell assay were used to evaluate the influence of NKTR on cell proliferation, migration, and invasion in vitro. Results: SAM yielded showed 256 up-regulated and 224 down-regulated differentially differently expressed genes. Seven genes were identified by using ADA, tools and four genes were verified by using qRT-PCR. Three genes (metastasis associated lung adenocarcinoma transcript 1 (MALAT1), nuclear factor I/B (NKTR), and nuclear factor I/B (NFIB)) showed a statistically significant considerably difference between CRC with and liver metastasis and CRC without liver metastasis. Immunohistochemical analysis showed that NKTR expression was much lower in primary CRC with liver metastasis than that in primary CRC without liver metastasis. The NKTR protein plays a role in the lytic function of natural killer (NK) cells and it has been rarely studied in the CRC. The down-regulation of NKTR by shRNA interference in CRC cells increased cell proliferation, migration, and invasion in vitro.

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Association of the expression of an SR-cyclophilin with myeloid cell differentiation

Cited by 7 publications

References 17 publications

Identification and comparative analysis of the peptidyl‐prolyl cis/trans isomerase repertoires of H. sapiens, D. melanogaster, C. elegans, S. cerevisiae and Sz. pombe

Identification and comparative analysis of the peptidyl‐prolyl cis/trans isomerase repertoires of H. sapiens, D. melanogaster, C. elegans, S. cerevisiae and Sz. pombe

A serine/arginine-rich nuclear matrix cyclophilin interacts with the C- terminal domain of RNA polymerase II

Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis

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