Association of TCF7L2 gene polymorphisms with susceptibility to type 2 diabetes mellitus in a Chinese Hui population

Abstract

ABSTRACT. Diabetes is one of costly chronic diseases. Previous studies across several ethnicities have shown that polymorphisms in the transcription factor 7-like 2 (TCF7L2) gene were strongly associated with susceptibility to type 2 diabetes (T2DM). In the present study, the association between the TCF7L2 gene and the susceptibility to T2DM in a Chinese Hui population was interrogated. Polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis and allelic specific PCR were employed for examining the TCF7L2 gene rs12255372 (G>T) and rs290487 (C>T), and rs7901695 (T>C) polymorphisms, respectively, in 109 healthy individuals and 111 subjects with T2DM who were of Chinese Hui descent and lived in the Ningxia Hui Autonomous Region of China. The results showed that the genotypic frequency of rs290487 and the allelic frequency distributions of the rs7901695 and rs290487 loci were not significantly different between patients and controls in this population. However, both the genotypic and the allelic frequencies at rs12255372 exhibited statistical 10065 TCF7L2 gene polymorphisms and T2DM©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 10064-10071 (2015) differences between the patients with T2DM and the unaffected cohort (P < 0.01). In addition, the frequency of the G allele at the rs12255372 locus in the patients was higher than that in healthy individuals (OR = 1.198, 95%CI = 1.097-1.307). These findings suggest that the TCF7L2 rs12255372 (G>T) polymorphism might be one of the most important genetic factors associated with T2DM susceptibility, and that individuals in the Chinese Hui population who carry a G allele at this locus might be at risk to develop T2DM.

Association between transcription factor 7-like 2 genetic polymorphisms and development of type 2 diabetes in a Chinese population

“…Assmann et al (2014), Guewo-Fokeng et al (2015), and Ouhaibi-Djellouli et al (2014) conducted investigations to assess the role of the TCF7L2 rs7903146 polymorphism in the pathogenesis of this disease, reporting that this sequence variant is associated with susceptibility to type 2 diabetes mellitus in Caucasians. In studies of Chinese populations, Yao et al (2015) and Yang et al (2015) determined that the TCF7L2 rs12255372 polymorphism may also contribute to the risk of developing this condition. However, some investigations have reached inconsistent conclusions (Barros et al, 2014;Pourahmadi et al, 2015).…”

“…To date, several studies have tested the association between TCF7L2 genetic variations and risk of developing type 2 diabetes mellitus (Assmann et al, 2014;Barros et al, 2014;OuhaibiDjellouli et al, 2014;Guewo-Fokeng et al, 2015;Nanfa et al, 2015;Yang et al, 2015;Yao et al, 2015). Assmann et al (2014), Guewo-Fokeng et al (2015), and Ouhaibi-Djellouli et al (2014) conducted investigations to assess the role of the TCF7L2 rs7903146 polymorphism in the pathogenesis of this disease, reporting that this sequence variant is associated with susceptibility to type 2 diabetes mellitus in Caucasians.…”

“…TCF7L2 expression is related to its proposed function in insulin secretion. Several previous studies have examined the role of the TCF7L2 rs7903146 polymorphism in the development of type 2 diabetes mellitus, but their results have been inconclusive (Assmann et al, 2014;Barros et al, 2014;Allahdini et al, 2015;GuewoFokeng et al, 2015;Liu et al, 2015;Nanfa et al, 2015;Yao et al, 2015;Yang et al, 2015). In this study, we conducted a hospital-based case-control investigation to evaluate the relationship between TCF7L2 rs7903146 and risk of developing type 2 diabetes mellitus in a Chinese population.…”

Integrative analysis of super enhancer SNPs for type 2 diabetes

“…Importantly, we identified that super enhancer SNPs were highly enriched (88/286) in TCF7L2 gene. Variants in TCF7L2 have been associated with T2D in multiple ethnic groups [ 58 – 60 ]. As a transcription factor, TCF7L2 plays a critical role in WNT signaling pathway and is involved in the development of various cell lineages and tissues [ 61 ].…”

“…Interestingly, among the 21 T2D super enhancer SNPs in adipose tissue, 13 SNPs were mapped to TCF7L2 gene. This gene encodes a WNT transcription factor which has been linked to a higher risk of developing T2D [ 58 – 60 , 66 ]. Previous studies have also indicated that WNT signaling pathway is the most commonly dysregulated process in myelogenous leukemia.…”