“…They could be simple amino acids, sugars, fatty acids or complex glycoproteins, hormones, etc. In our previous study (Behera et al., 2020), the level of taurine was reported to be lower in the plasma during postpartum anestrus conditions in buffaloes. Therefore, to explore further, the present study was targeted to understand serum taurine dynamics during the oestrous cycle in buffaloes.…”
Section: Discussionmentioning
confidence: 75%
“…In addition, it is a known fact that estrogens are synthesised from their precursors like testosterone. In our previous study, we found a positive correlation between taurine and testosterone in the follicular fluid of developing ovarian follicles (Behera et al, 2020). Therefore, a greater concentration of taurine just before estrus (proestrus) could also contribute to the greater concentration of testosterone during proestrus, which is the F I G U R E 8 ROC curve analysis for serum proline concentration.…”
Section: Notementioning
confidence: 89%
“…After the oestrous cycle stages were determined, four samples representing all four stages of the oestrous cycle were taken from each animal. The serum samples were deproteinised by adding 400 μL of methanol to 100 μL serum (sample:methanol::1:4) as per the protocol established in our lab earlier (Behera et al., 2020) with little modifications from a previous publication (Mu et al., 2019). The mixture was incubated at room temperature for about 6 min and centrifuged at 8000 g for 5 min at 4°C.…”
Section: Methodsmentioning
confidence: 99%
“…After the spots dried, 10 μL of 1 mg/mL proline was added to the same spots externally. Proline solution was used as a biological control to minimise the plate variations as given in our previous protocol, which was performed on ovarian follicular fluid and the blood plasma from post-partum buffaloes (Behera et al, 2020). Along with the serum samples, 10 μL of standard (1 mg/ mL) taurine was also loaded on the plate as a separate spot to compare the relative spots of taurine obtained from the serum samples.…”
Section: Tlc For Quantification Of Taurine In Buffalo Serummentioning
confidence: 99%
“…Further, taurine also showed a positive correlation (0.75) with testosterone and a negative correlation (−0.77) with oestrogen in the developing ovarian follicles of buffaloes, indicating the importance of taurine and testosterone during the development of ovarian follicles. Apart from ovarian follicular fluid, a significantly lower level of taurine in the plasma of buffalo during post‐partum anestrus condition shows not only taurine's role in maintaining normal reproductive cyclicity in buffaloes (Behera et al., 2020) but also highlights the necessity to understand the plasma or serum dynamics of taurine during the reproductive cycle of buffaloes. However, serum taurine dynamics during the buffalo oestrous cycle were not reported.…”
Estrus identification is one of the common issues in buffaloes because of their short estrus duration and silent estrus problem. Hence, specific biomarkers facilitating in identifying the estrus stage would be helpful to buffalo farmers and researchers. In our previous studies, taurine, a non‐protein amino acid that helps in the secretion of reproductive hormones such as GnRH, was found to be associated with postpartum anestrus in buffaloes. Therefore, the present study was conducted to explore the level of taurine in serum during different stages of the oestrous cycle in healthy cyclic buffaloes. Blood samples were collected from healthy cyclic buffaloes (n = 4), and taurine was estimated at the estrus (0th day), proestrus (−2nd day), metestrus (3rd day) and diestrus (+10th day) stages using TLC method. The days of the oestrous cycle were determined by ultrasonography and observation of behavioural signs by trained professionals. The results revealed that taurine was consistently present in the serum. However, the highest concentration of taurine was observed at the proestrus (0.20 ± 0.03 mg/mL) stage, which was greater (p < .05) than metestrus (0.10 ± 0.05 mg/mL) and diestrus (0.13 ± 0.03 mg/mL) stages, but comparable with the estrus stage. These results were also validated in the simulated population datasets of population size 6 to 10,000. Further, ROC curve analysis for the large simulated population indicated the efficiency of taurine to distinguish proestrus from metestrus and diestrus stages at a lower cutoff value of <0.1643 mg/mL with 60% sensitivity and specificity. Therefore, the present study concludes that serum taurine concentration could help in detecting proestrus stage of buffalo estrous cycle.
“…They could be simple amino acids, sugars, fatty acids or complex glycoproteins, hormones, etc. In our previous study (Behera et al., 2020), the level of taurine was reported to be lower in the plasma during postpartum anestrus conditions in buffaloes. Therefore, to explore further, the present study was targeted to understand serum taurine dynamics during the oestrous cycle in buffaloes.…”
Section: Discussionmentioning
confidence: 75%
“…In addition, it is a known fact that estrogens are synthesised from their precursors like testosterone. In our previous study, we found a positive correlation between taurine and testosterone in the follicular fluid of developing ovarian follicles (Behera et al, 2020). Therefore, a greater concentration of taurine just before estrus (proestrus) could also contribute to the greater concentration of testosterone during proestrus, which is the F I G U R E 8 ROC curve analysis for serum proline concentration.…”
Section: Notementioning
confidence: 89%
“…After the oestrous cycle stages were determined, four samples representing all four stages of the oestrous cycle were taken from each animal. The serum samples were deproteinised by adding 400 μL of methanol to 100 μL serum (sample:methanol::1:4) as per the protocol established in our lab earlier (Behera et al., 2020) with little modifications from a previous publication (Mu et al., 2019). The mixture was incubated at room temperature for about 6 min and centrifuged at 8000 g for 5 min at 4°C.…”
Section: Methodsmentioning
confidence: 99%
“…After the spots dried, 10 μL of 1 mg/mL proline was added to the same spots externally. Proline solution was used as a biological control to minimise the plate variations as given in our previous protocol, which was performed on ovarian follicular fluid and the blood plasma from post-partum buffaloes (Behera et al, 2020). Along with the serum samples, 10 μL of standard (1 mg/ mL) taurine was also loaded on the plate as a separate spot to compare the relative spots of taurine obtained from the serum samples.…”
Section: Tlc For Quantification Of Taurine In Buffalo Serummentioning
confidence: 99%
“…Further, taurine also showed a positive correlation (0.75) with testosterone and a negative correlation (−0.77) with oestrogen in the developing ovarian follicles of buffaloes, indicating the importance of taurine and testosterone during the development of ovarian follicles. Apart from ovarian follicular fluid, a significantly lower level of taurine in the plasma of buffalo during post‐partum anestrus condition shows not only taurine's role in maintaining normal reproductive cyclicity in buffaloes (Behera et al., 2020) but also highlights the necessity to understand the plasma or serum dynamics of taurine during the reproductive cycle of buffaloes. However, serum taurine dynamics during the buffalo oestrous cycle were not reported.…”
Estrus identification is one of the common issues in buffaloes because of their short estrus duration and silent estrus problem. Hence, specific biomarkers facilitating in identifying the estrus stage would be helpful to buffalo farmers and researchers. In our previous studies, taurine, a non‐protein amino acid that helps in the secretion of reproductive hormones such as GnRH, was found to be associated with postpartum anestrus in buffaloes. Therefore, the present study was conducted to explore the level of taurine in serum during different stages of the oestrous cycle in healthy cyclic buffaloes. Blood samples were collected from healthy cyclic buffaloes (n = 4), and taurine was estimated at the estrus (0th day), proestrus (−2nd day), metestrus (3rd day) and diestrus (+10th day) stages using TLC method. The days of the oestrous cycle were determined by ultrasonography and observation of behavioural signs by trained professionals. The results revealed that taurine was consistently present in the serum. However, the highest concentration of taurine was observed at the proestrus (0.20 ± 0.03 mg/mL) stage, which was greater (p < .05) than metestrus (0.10 ± 0.05 mg/mL) and diestrus (0.13 ± 0.03 mg/mL) stages, but comparable with the estrus stage. These results were also validated in the simulated population datasets of population size 6 to 10,000. Further, ROC curve analysis for the large simulated population indicated the efficiency of taurine to distinguish proestrus from metestrus and diestrus stages at a lower cutoff value of <0.1643 mg/mL with 60% sensitivity and specificity. Therefore, the present study concludes that serum taurine concentration could help in detecting proestrus stage of buffalo estrous cycle.
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