Association of Target Therapy Gene Expression with Metastasizing of Clear-Cell Renal Cell Carcinoma

Apanovich, Natalya; Peters, M. V.; Апанович, П. В.; Маркова, А. С.; Камолов, Б. Ш.; Матвеев, В. Б.; Карпухин, А. В.

doi:10.1007/s10517-018-4327-z

Cited by 2 publications

(2 citation statements)

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“…In addition, over-expression of MCM3AP-AS1 significantly augmented the proliferation, tube formation ability of Caki-1 and 786-O cells and the expressions of Ki67, VEGF-A, TNF-α, IL-1β, and IL-6 in ccRCC Caki-1 and 786-O cells, while silencing MCM3AP-AS1 brought about the opposite results. Consistent with our findings, inhibited expression of the VEGFA gene is correlated with reduction in the time to ccRCC metastasis appearance (29). A previous study also noted that diminished VEGFA suppressed the proliferation, migration and invasion of ccRCC 786-O cells, while promoting their apoptosis (30).…”

Section: Discussionsupporting

confidence: 91%

Pro-Angiogenic and Pro-Inflammatory Regulation by lncRNA MCM3AP-AS1-Mediated Upregulation of DPP4 in Clear Cell Renal Cell Carcinoma

Qiu

Yang

et al. 2020

Front. Oncol.

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Clear cell renal cell carcinoma (ccRCC) represents the most common type of renal cell carcinoma (RCC) in adults, in addition to the worst prognosis among the common epithelial kidney tumors. Inflammation and angiogenesis seem to potentiate tumor growth and metastasis of the malignancy. The current study explored the contributions of the lncRNA MCM3AP-AS1 in tumor-associated inflammation and angiogenesis in ccRCC with a specific focus on its transcriptional regulation and its interactions with transcription factor E2F1 and DPP4. Tumor tissues and matched adjacent non-tumor tissues were collected from 78 ccRCC patients. Methylation-specific PCR and ChIP assays were applied to detect the methylation at the promoter region of MCM3AP-AS1. Dual-luciferase reporter assay, RIP, RNA pull-down, and ChIP assays were employed to confirm the interactions between MCM3AP-AS1, E2F1, and DPP4. Nude mice were subcutaneously xenografted with human ccRCC cells. Cell proliferation was evaluated by CCK-8 assays and EDU staining in ccRCC cells in vitro and by immunohistochemical staining of Ki67 in vivo. Inflammation was examined by detecting the secretion of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6). Pro-angiogenic ability of ccRCC cells was assessed by the co-culture with human umbilical vein endothelial cells (HUVEC) in vitro and by microvessel density (MVD) measurements and angiogenesis in the chicken chorioallantoic membrane. MCM3AP-AS1 was highly-expressed in ccRCC and associated with poor patient survival. Demethylation of MCM3AP-AS1 was noted in ccRCC tissues and cells. Over-expression of MCM3AP-AS1 enhanced cell proliferation, the release of pro-inflammatory cytokines, and the tube formation of HUVECs in cultured human Caki-1 and 786-O cells. MCM3AP-AS1 was shown to enhance the E2F1 enrichment at the DPP4 promoter, to further increase the expression of DPP4. Knockdown of DPP4 could abate pro-angiogenic and pro-inflammatory abilities of MCM3AP-AS1 in ccRCC cells. Pro-angiogenic and pro-inflammatory abilities of MCM3AP-AS1 in vivo were confirmed in mice subcutaneously xenografted with human ccRCC cells. Our findings demonstrate a novel mechanism by which lncRNA MCM3AP-AS1 exerts pro-angiogenic and pro-inflammatory effects, highlighting the potential of MCM3AP-AS1 as a promising target for treating ccRCC.

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Section: Discussionsupporting

confidence: 91%

Pro-Angiogenic and Pro-Inflammatory Regulation by lncRNA MCM3AP-AS1-Mediated Upregulation of DPP4 in Clear Cell Renal Cell Carcinoma

Qiu

Yang

et al. 2020

Front. Oncol.

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“…Проблема поиска маркеров метастазирования достаточно значима, так как фактически они являются прогнозом метастатических рецидивов. В литературе имеются сообщения о маркерах диагностики и прогноза метастазирования ПКР на основе анализа экспрессии генов, в том числе выполненные авторами настоящей работы [26][27][28][29], а также на основе экспрессии миРНК, например miR-429, или миРНК семейства miR-200 [30,31]. Набор из 3 миРНК (miR-21, -142-3p, -194) позволил прогнозировать метастазирование ПКР с чувствительностью 86,7 % и специфичнос тью 82,0 % [32].…”

Section: рис 2 ассоциация метилирования генов микрорнк с клинико-гиunclassified

Methylation of a group of microRNA genes: markers of renal cell carcinoma metastasis

et al. 2021

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Почечно-клеточный рак (ПКР) не имеет симптомов вплоть до поздних стадий и характеризуется высокой частотой летальных исходов, достигающей 90 % при развитии метастатического процесса. Цель исследования-определение группы генов микроРНК, метилирование которых ассоциировано с прогрессированием заболевания, в частности с метастазированием.

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Association of Target Therapy Gene Expression with Metastasizing of Clear-Cell Renal Cell Carcinoma

Cited by 2 publications

References 5 publications

Pro-Angiogenic and Pro-Inflammatory Regulation by lncRNA MCM3AP-AS1-Mediated Upregulation of DPP4 in Clear Cell Renal Cell Carcinoma

Pro-Angiogenic and Pro-Inflammatory Regulation by lncRNA MCM3AP-AS1-Mediated Upregulation of DPP4 in Clear Cell Renal Cell Carcinoma

Methylation of a group of microRNA genes: markers of renal cell carcinoma metastasis

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