Association of soluble HLA‐G plasma levels with HLA‐G alleles

Rebmann, Vera; Ven, Katrin van der; Pässler, M; Pfeiffer, Kerstin; Krebs, D.; Grosse‐Wilde, H.

doi:10.1034/j.1399-0039.2001.057001015.x

Cited by 186 publications

(177 citation statements)

References 41 publications

(42 reference statements)

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“…Moreover, it was shown that in M8 cells treated with arsenite, HLA-G6 was the first transcript induced (40). It thus appears that HLA-G transcripts that contain intron 4 might be preferentially expressed after HLA-G gene activation in some cell lines, suggesting tight control of HLA-G alternative splicing, which in part may be linked to the HLA-G allelic form (65). For example, we can postulate that mutations in HLA-G gene splicing sites might favor the expression of intron 4-containing transcripts in some cell lines.…”

Section: Discussionmentioning

confidence: 99%

HLA-G gene repression is reversed by demethylation

Moreau

Mouillot

Rousseau

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

140

134

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The HLA-G molecule plays an important role in immune tolerance, protecting the fetus from maternal immune attack, and probably contributes to graft tolerance and tumor escape from the host immune system. HLA-G expression is tightly regulated and involves mechanisms acting in part at the transcriptional level. Nevertheless, almost all regulatory sequences that govern constitutive and inducible HLA class I gene transcription are disrupted in the HLA-G gene promoter, suggesting an unusual regulatory process. In further investigating the molecular mechanisms of HLA-G gene activation, we evaluated the influence of epigenetic mechanisms on seven HLA-G-negative cell lines that exhibit various phenotypes. Exposure of cells to histone deacetylase inhibitors, or to the demethylating agent 5-aza-2 -deoxycytidine, revealed that HLA-G gene transcription is inhibited by DNA methylation. Reversal of methylation-mediated repression may directly induce HLA-G cellsurface expression, supporting the idea that HLA-G might be activated by such a mechanism during malignancy, inflammation, and allogenic reactions.

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Section: Discussionmentioning

confidence: 99%

HLA-G gene repression is reversed by demethylation

Moreau

Mouillot

Rousseau

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

140

134

View full text Add to dashboard Cite

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“…However, we cannot exclude that HLA-G-positive patients may carry out specific HLA-G alleles associated with high HLA-G production. 30 In addition, the degree of histoincompatibility between donor and recipient may play a role in stimulating HLA-G production. Today, heart transplantation has been established as a valuable therapeutic approach to end-stage heart failure.…”

Section: Discussionmentioning

confidence: 99%

Human Leukocyte Antigen-G Expression After Heart Transplantation Is Associated With a Reduced Incidence of Rejection

et al. 2002

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Background — Human leukocyte antigen (HLA)-G, a nonclassic major histocompatibility complex class I molecule expressed in the extravillous cytotrophoblast at the feto-maternal interface, is known to protect the fetus from maternal cellular immunity. In a preliminary study, we showed that HLA-G is expressed in the hearts of some patients after heart transplantation. Methods and Results — In the present study, a larger number of patients was investigated to confirm this finding and to look for possible correlations between HLA-G expression and the number and types of rejection. Expression of HLA-G in endomyocardial biopsy specimens was investigated by immunohistochemical analysis, and detection of the soluble HLA-G in the serum was performed by immunoprecipitation followed by Western blot analysis. HLA-G was detected in the biopsy specimens and serum of 9 of 51 patients (18%). The number of episodes of acute rejection was significantly lower in HLA-G-positive patients (1.2±1.1) as compared with HLA-G-negative patients (4.5±2.8) ( P <0.001). No chronic rejection was observed in HLA-G-positive patients, whereas 15 HLA-G-negative patients had chronic rejection ( P <0.032). A longitudinal study of these patients reveals that the status of HLA-G expression was maintained after 6 months both in serum and in biopsy specimens. During this period, HLA-G-positive patients did not have chronic rejection. Conclusions — There is a significant correlation between rejection and HLA-G expression in the heart after transplantation. HLA-G expression and its effect in reducing the incidence and severity of rejection seem to be stable throughout the evolution.

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“…19 In this study, the HLA-G*0103 was associated with protection against HPV lesions when patients considered as a whole were compared to controls. In addition, all the individuals presenting the G*0103 allele in this study were homozygous for this allele (confirmed by sequencing analysis), indicating that the associations with the G*0103 allele or G*0103/G*0103 genotype were not independent observations.…”

Section: Hla-g In Patients Stratified According To Lesion Severity Anmentioning

confidence: 99%

“…18 HLA-G alleles may influence the plasma levels of soluble HLA-G (sHLA-G). 19 In addition, a 14 bp insertion/deletion polymorphism has been reported in the 3 0 -untranslated region (UTR) of exon 8. 20 HLA-G alleles exhibiting the 14 bp insertion ( þ 14 bp) undergo an alternative splicing that removes 92 bases from 3 0 UTR, 21 influencing the stability of HLA-G mRNA.…”

Section: -15mentioning

confidence: 99%

HLA-G polymorphisms in women with squamous intraepithelial lesions harboring human papillomavirus

et al. 2009

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Human papillomavirus (HPV) infection is etiologically associated with low-(LSIL) and high-grade squamous intraepithelial lesions (HSIL) and with cervical cancer. The progression or regression of the lesions may depend, among other factors, on the host heritable immune response. Because human leukocyte antigen (HLA)-G molecules are involved in the modulation of innate and adaptive immune responses, and because no previous studies have evaluated HLA-G polymorphism in patients with SIL, we conducted a study to assess the association between HLA-G polymorphisms and cervical lesions harboring HPV infection. Cervico-vaginal scrapings and blood samples were collected from 125 women with SIL (68 LSIL and 57 HSIL) and from 94 healthy women without HPV infection and cytological abnormalities. HPV type and HLA-G polymorphisms in exons 2, 3 and 8 (14 bp insertion/deletion) were evaluated by PCR methodology, and digested with restriction endonucleases. The Genepop software and the EM and PHASE algorithms were used for statistical analysis. A significant protective association was observed between the presence of the G*0103 allele and SIL and between the G0101/G0104 genotype and HSIL in the group of patients compared to control. The presence of the G0104/ þ 14 bp and G0104/À14 bp haplotypes conferred susceptibility to SIL compared to control. In addition, patients possessing the G0104/ þ 14 bp haplotype and harboring HPV-16 and -18 co-infections were particularly associated with HSIL. These findings suggest that HLA-G polymorphisms may be associated with HPV infection and SIL, consequently representing a profile of predisposition to cervical cancer.

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Association of soluble HLA‐G plasma levels with HLA‐G alleles

Cited by 186 publications

References 41 publications

HLA-G gene repression is reversed by demethylation

HLA-G gene repression is reversed by demethylation

Human Leukocyte Antigen-G Expression After Heart Transplantation Is Associated With a Reduced Incidence of Rejection

HLA-G polymorphisms in women with squamous intraepithelial lesions harboring human papillomavirus

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