Association of SNP (-G1082A)IL-10with Increase in Severity of Periportal Fibrosis in Schistosomiasis, in the Northeast of Brazil

Abstract

Interleukin 10 (IL-10) is an important anti-inflammatory cytokine that modulates severe periportal fibrosis (PPF). We hypothesized that genetic polymorphisms (-G1082A/-C819T/-C592A) of the IL-10 gene and classic factors (age, sex, alcohol, exposure, and specific treatment) are associated with the severity of PPF and that these polymorphisms influence IL-10 expression. In this cross-sectional study, we genotyped these polymorphisms within the IL-10 gene in 203 Brazilian subjects infected with Schistosoma mansoni, with different patterns of PPF. There was an association of protection between the ages of 41 and 60 years and advanced standard PPF. The -1082AA genotype was significantly associated with severity in PPF when compared with the -1082GG genotype. Similarly, when analyzed together, both the -1082GA+AA genotypes were significantly associated. The ACC and GTA haplotypes indicated a protective effect against PPF, while the ATA haplotype was significantly associated with PPF severity when compared with the GCC haplotype. There was no significant difference between average levels of IL-10 between clinical groups, and there was no association between average serum levels of IL-10 and (-G1082A) IL-10 polymorphism. Our results suggest that (-G1082A) IL-10 polymorphism and putative haplotypes are associated with PPF severity in the Brazilian population.

Differential contribution of interleukin‐10 promoter variants in malaria and schistosomiasis mono‐ and co‐infections among Nigerian children

“…A study investigated the association of the three IL-10 promoter variants -1082G/ A, -819C/T and -592C/A with the severity of periportal fibrosis in schistosomiasis. The results showed that the -1082A/G SNP and haplotypes ACC, GTA and ATA were associated with fibrosis severity [29]. Minor alleles of the three promoter SNPs were found associated with total IgE levels and S. mansoni infection intensity in a schistosomiasis-endemic area in Brazil [30].…”

There is no evident correlation between interleukin-10 gene polymorphisms and periportal fibrosis regression after specific treatment

“…Additionally, -G1082A/-C819T/-C592A IL-10 polymorphisms and their respective serum concentrations determined for these patients (9) . The present cohort study included 119 patients infected with S. mansoni who were recruited during 2012-2013 and followed-up retrospectively for 2 years after specifi c treatment for S. mansoni to estimate the probability of PPF regression.…”

“…In the previous study (9) with 203 patients, polymorphisms -G1082A/-C819T/-C592A IL-10 in the promoter region of the IL-10 gene were determined alongside their respective serum concentrations. Of the 203 patients in this previous study, the current study excluded 12 who had image pattern A (without PPF) in the fi rst abdominal ultrasound, 66 who did not have a follow-up of 2 years after the fi rst specifi c treatment for S. mansoni, and 6 for whom the genotype frequencies for regions (-C819T/-C592A) of the IL-10 gene were not determined.…”

“…The main exposures were polymorphisms -G1082A/-C819T/-C592A IL-10 which, in the context of the hypothesis of this study, were investigated for associations with the severity of PPF. The information on these genotypes and their respective serum IL-10 concentrations were obtained in 2012 and 2013 (9) .…”

“…Additionally, there was no difference in serum levels of IL-10 between exposure groups. Recently, Silva et al (9) evaluated the effects of these polymorphisms on the severity of PPF in 203 patients infected with S. mansoni who had different patterns of PPF in the State of Pernambuco. Silva et al found that only polymorphism (-G1082A) showed risk associations with disease severity in this Brazilian population.…”