Association of smoking, alcohol and NSAIDs use with expression of cag A and cag T genes of Helicobacter pylori in salivary samples of asymptomatic subjects

Ghosh, Pranab; Bodhankar, Subhash L.

doi:10.1016/s2221-1691(12)60080-x

Cited by 14 publications

(8 citation statements)

References 35 publications

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“…Some previous studies have reported significantly higher prevalences of H. pylori in males than in females [ 34 , 35 ]. Others have found no association with gender [ 11 , 15 ], a result that agrees with our findings. Higher prevalences have been reported previously in cattlemen and agriculturists, suggesting possible zoonotic transmission [ 26 ].…”

Section: Discussionsupporting

confidence: 93%

“…Previous estimates of the prevalence of H. pylori infection in stool samples of asymptomatic persons have ranged from 6.8% to 73.3% [ 10 – 14 ]. Corresponding values for saliva samples are 45.7%–67.8% [ 10 , 11 , 15 ]. In Thailand, only one study has so far investigated the prevalence of H. pylori infection in stool samples of asymptomatic persons [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes

et al. 2018

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BackgroundTwo-thirds of the world’s population is thought to be infected by Helicobacter pylori. Although most people infected with H. pylori are asymptomatic, this pathogen is associated with several gastric pathologies including cancer. The risk factors for colonization are still unclear and the genetic diversity within individual hosts has never been clearly investigated.ResultThis study determined the prevalence of, and explored risk factors for, H. pylori infection directly from paired saliva (n = 110) and stool (n = 110) samples from asymptomatic persons in Northeast Thailand. Samples were subjected to indirect immunofluorescence assay (IFA), 16S rRNA-based real-time PCR and vacA-based semi-nested PCR. Partial vacA gene sequences of H. pylori were compared between saliva and stool samples.The overall prevalence of H. pylori infection in our asymptomatic study population was 64%. Age, gender, occupation and frequency of brushing teeth were not found to be associated with H. pylori colonization. The vacA gene was successfully sequenced from both saliva and stool samples of 12 individuals. For seven of these individuals, saliva and stool sequences fell into different clusters on a phylogenetic tree, indicating intra-host genetic variation of H. pylori.ConclusionThis study reports a high prevalence of H. pylori infection in asymptomatic persons in this region of Thailand and demonstrates that genotypes (vacA gene sequences) of H. pylori may differ between the oral cavity and intestinal tract.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1150-7) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes

et al. 2018

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“…This is due to the fact that smoking causes various ulcers in the respiratory and digestive tracts, which can lead to infection and gastric cancer. 19,20 Likewise, a significant relationship was found in our study between H. pylori infection and smoking (P= 0.027). In terms of antibiotics, it seems that the use of antibiotics results in cross reactions and, subsequently, in false positive results in ELISA test.…”

Section: Comparison Of Elisa and Histology Resultssupporting

confidence: 59%

Challenges of Accurate Diagnosis of Helicobacter pylori in Iran

Fotoohi

Kazemi

Ansariniya

2019

Int J Enteric Pathog

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Background: Helicobacter pylori-associated gastritis is regarded as the most prevalent gastrointestinal infection, as well as the major cause of several other diseases. Despite being discovered over 30 years ago, no golden diagnostic method has yet been suggested for this microorganism. Objective: The present study aimed to compare two common diagnostic methods of histology and serology. Materials and Methods: This cross-sectional study began in July, 2016, and was conducted at Imam Jafar Sadegh Hospital (a university hospital) in Maybod, Yazd, Iran, on 70 patients. All the patients underwent endoscopy, and biopsy samples were taken for histology, as well as blood samples for ELISA test. Finally, sensitivity, specificity, NPV (negative predictive values), and PPV (positive predictive values) were calculated and analyzed. Results: The sensitivity, specificity, accuracy, PPV, and NPV of the ELISA method with histology as the gold standard were 87.5%, 36.9%, 54.2%, 42%, and 85%, respectively. Still, the histology method appears to be the most reliable test for the diagnosis of H. pylori in Iran. Conclusion: Regarding the high prevalence of H. pylori in Iran, the present study recommends the use of histology as a diagnostic method in combination with other diagnostic methods for detecting H. pylori infection.

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“…Smoking might be a major risk factor entailed in modulating the susceptibility of an individual suffering with ulcers to H. pylori infection. Evidently, study on H. pylori positive asymptomatic and symptomatic subjects with acid peptic disease showed that the risk of virulent infection was escalated by smoking in both groups of individual 33 . Statistically significant association was observed (Pvalue=0.030) in the prevalence of H. pylori infection and type of occupations in this study which is in line with other studies 34,35 .…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Helicobacter Pylori Among Asymptomatic Populations in Sana'a, Yemen

Alyahawi

Al-kaf

Alzaghrori

2018

UJPR

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Helicobacter pylori consider the most important cause of chronic gastritis and also the most important etiological factor responsible for the duodenal and gastric ulcer and have an important role in the pathogenesis of gastric cancer. The aim of the present study to know the prevalence of H. pylori among the khat chewing adults in compared to non-khat chewing among asymptomatic populations. A total of 82 healthy subjects were screened to find out the prevalence of Helicobacter pylori using stool antigen card test during the period started in 16 April 2018 and ending in 8 May 2018 in Sana'a, Yemen. Predesigned questionnaire was used to collect information such as age, sex, education status, khat chewing, smoking, tobacco chewing, occupation, and tests results all above data were recorded for each subject. Patients who had taken proton pump inhibitors or antibiotic for a month prior to study were excluded. Out of these 82 asymptomatic individuals, 36 were found positive for Helicobacter pylori by the test, giving a prevalence of 43.9 %. A prevalence of 26.9 % (62.9 % of khat chewing) was khat chewing individuals and it had statistically significant difference. A prevalence of 14.6 % was seen among cigarette smoking participants. The present study revealed substantial prevalence of Helicobacter pylori in khat chewing healthy subjects. A higher prevalence of Helicobacter pylori seen in these subjects may be contributed to khat chewing. Identification of khat chewing populations, who do not show symptoms of Helicobacter pylori infection, is essential for controlling the infection and it still remains a challenge for the clinicians.

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Association of smoking, alcohol and NSAIDs use with expression of cag A and cag T genes of Helicobacter pylori in salivary samples of asymptomatic subjects

Cited by 14 publications

References 35 publications

Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes

Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes

Challenges of Accurate Diagnosis of Helicobacter pylori in Iran

Prevalence of Helicobacter Pylori Among Asymptomatic Populations in Sana'a, Yemen

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