Association of Short‐Chain Fatty Acids with Gut Microbiota and Lipid Metabolism in Mice with Diarrhea Induced by High‐Fat Diet in a Fatigued State

Qiao, Bo; Liu, Jing; Peng, Xinxin; Cai, Ying; Peng, Maijiao; Li, Xiaoya; Tan, Zhoujin; Deng, Na

doi:10.1002/mnfr.202300452

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…Structure and functional changes in the commensal gut microbiota are thought to be involved in the pathogenesis of many diseases ( Parker et al, 2022 ; Qiao et al, 2023 ). Several studies have elucidated that the bidirectional communication between the eye diseases and the gastrointestinal microbiota, indicating an accurate characterization of the gut microbiome is of critical importance for maintaining ocular health.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiome and plasma metabolome alterations in myopic mice

Li,

Liu,

Zhang

et al. 2023

Front. Microbiol.

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BackgroundMyopia is one of the most common eye diseases leading to blurred distance vision. Inflammatory diseases could trigger or exacerbate myopic changes. Although gut microbiota bacteria are associated with various inflammatory diseases, little is known about its role in myopia.Materials and methodsThe mice were randomly divided into control and model groups, with the model group being attached-30D lens onto the eyes for 3 weeks. Then, mouse cecal contents and plasma were collected to analyze their intestinal microbiota and plasma metabolome.ResultsWe identified that the microbial composition differed considerably between the myopic and non-myopic mice, with the relative abundance of Firmicutes phylum decreased obviously while that of Actinobacteria phylum was increased in myopia. Furthermore, Actinobacteria and Bifidobacterium were positively correlated with axial lengths (ALs) of eyeballs while negatively correlated with refractive diopters. Untargeted metabolomic analysis identified 141 differentially expressed metabolites, and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed considerable enrichment mainly in amino acid metabolism pathways. Notably, pathways involved glutamate metabolism including “Glutamine and D-glutamate metabolism” and “Alanine, aspartate and glutamate metabolism” was changed dramatically, which presented as the concentrations of L-Glutamate and L-Glutamine decreased obviously in myopia. Interestingly, microbiome dysbiosis and metabolites alternations in myopia have a disrupting gut barrier feature. We further demonstrated that the gut barrier function was impaired in myopic mice manifesting in decreased expression of Occludin, ZO-1 and increased permeation of FITC-dextran.DiscussionMyopic mice had obviously altered gut microbiome and metabolites profiles compared to non-myopic mice. The dysbiosis and plasma metabolomics shift in myopia had an interrupting gut barrier feature. Our study provides new insights into the possible role of the gut microbiota in myopia and reinforces the potential feasibility of microbiome-based therapies in myopia.

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Section: Discussionmentioning

confidence: 99%

Gut microbiome and plasma metabolome alterations in myopic mice

Li,

Liu,

Zhang

et al. 2023

Front. Microbiol.

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“…Disruption of the microbiota structure can reduce the production of beneficial metabolites and increase the accumulation of harmful metabolites. In diarrhea mice induced by a high-fat diet under fatigue, there is a reduction in the production of short-chain fatty acids (SCFAs) ( Qiao et al, 2023 ). Similarly, in diabetic kidney disease patients with imbalanced intestinal microbiota, the production of SCFAs decreases, and the levels of harmful metabolites such as TMAO increase ( Tian et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Dysfunction of cecal microbiota and CutC activity in mice mediating diarrhea with kidney-yang deficiency syndrome

Guo,

Fang,

Chen

et al. 2024

Front. Microbiol.

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ObjectivePrevious studies have indicated that diarrhea with kidney-yang deficiency syndrome leads to a disorder of small intestine contents and mucosal microbiota. However, the relationship of TMA-lyase (CutC) activity and TMAO with diarrhea with kidney-yang deficiency syndrome remains unexplored. Therefore, this study explores the relationship between cecal microbiota and choline TMA-lyase (CutC) activity, as well as the correlation between trimethylamine oxide (TMAO), inflammatory index, and CutC activity.MethodTwenty SPF-grade male KM mice were randomly divided into the normal group (CN) and the diarrhea model group (CD). Diarrhea mouse models were established by adenine combined with Folium sennae administration. CutC activity, TMAO, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels were detected, and the cecal content microbiota was sequenced.ResultAfter 14 days, diarrhea occurred in the CD group. Compared with the CN group, there was no significant change in the activity of CutC in the small intestine of the CD group, while the activity of CutC in the cecum was significantly increased, and the levels of TMAO, IL-6, and TNF-α showed a significant increase. The Chao1 index, Observed_species index, Shannon index, and Simpson index all exhibited a decreasing trend. The main changes at the bacterial genus level were Alistipes, Enterorhabdus, Desulfovibrio, Bacteroides, Candidatus_Saccharimonas, and [Ruminococcus]_torques_group. The results of LEfSe analysis, random forest analysis and ROC curve analysis revealed Paludicola, Blautia, Negativibacillus, Paraprevotella, Harryflintia, Candidatus_Soleaferrea, Anaerotruncus, Oscillibacter, Colidextribacter, [Ruminococcus]_torques_group, and Bacteroides as characteristic bacteria in the CD group. Correlation analysis showed a significant negative correlation between cecal CutC activity and Ligilactobacillus, and a significant positive correlation with Negativibacillus and Paludicola. The level of TMAO was significantly positively correlated with CutC activity and IL-6.ConclusionDiarrhea with kidney-yang deficiency syndrome significantly affects the physiological status, digestive enzyme activity, CutC activity, TMAO levels, and inflammatory response in mice. Additionally, there are changes in the composition and function of cecal microbiota, indicating an important impact of diarrhea with kidney-yang deficiency syndrome on the host intestinal microbiota balance. The occurrence of diarrhea with kidney-yang deficiency syndrome may be associated with dysbiosis of intestinal microbiota, increased CutC activity, elevated TMAO levels, and heightened inflammatory factor levels.

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“…Understanding SMF involves recognizing it as a symptom rather than a standalone condition. Alongside treating the root cause, patients may benefit from general strategies to reduce fatigue, such as regular physical activity, balanced nutrition, stress management techniques, and pacing daily activities to avoid overexertion ( 17 ). Effectively managing SMF requires a comprehensive, individualized approach that targets the specific underlying cause while also incorporating general strategies to improve overall energy levels and well-being.…”

Section: Introductionmentioning

confidence: 99%

Renshen Yangrong decoction for secondary malaise and fatigue: network pharmacology and Mendelian randomization study

Wang,

Zhu,

Cui

et al. 2024

Front. Nutr.

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BackgroundRenshen Yangrong decoction (RSYRD) has been shown therapeutic effects on secondary malaise and fatigue (SMF). However, to date, its bioactive ingredients and potential targets remain unclear.PurposeThe purpose of this study is to assess the potential ingredients and targets of RSYRD on SMF through a comprehensive strategy integrating network pharmacology, Mendelian randomization as well as molecular docking verification.MethodsSearch for potential active ingredients and corresponding protein targets of RSYRD on TCMSP and BATMAN-TCM for network pharmacology analysis. Mendelian randomization (MR) was performed to find therapeutic targets for SMF. The eQTLGen Consortium (sample sizes: 31,684) provided data on cis-expression quantitative trait loci (cis-eQTL, exposure). The summary data on SMF (outcome) from genome-wide association studies (GWAS) were gathered from the MRC-IEU Consortium (sample sizes: 463,010). We built a target interaction network between the probable active ingredient targets of RSYRD and the therapeutic targets of SMF. We next used drug prediction and molecular docking to confirm the therapeutic value of the therapeutic targets.ResultsIn RSYRD, network pharmacology investigations revealed 193 possible active compounds and 234 associated protein targets. The genetically predicted amounts of 176 proteins were related to SMF risk in the MR analysis. Thirty-seven overlapping targets for RSYRD in treating SMF, among which six (NOS3, GAA, IMPA1, P4HTM, RB1, and SLC16A1) were prioritized with the most convincing evidence. Finally, the 14 active ingredients of RSYRD were identified as potential drug molecules. The strong affinity between active components and putative protein targets was established by molecular docking.ConclusionThis study revealed several active components and possible RSYRD protein targets for the therapy of SMF and provided novel insights into the feasibility of using Mendelian randomization for causal inference between Chinese medical formula and disease.

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Association of Short‐Chain Fatty Acids with Gut Microbiota and Lipid Metabolism in Mice with Diarrhea Induced by High‐Fat Diet in a Fatigued State

Cited by 8 publications

References 40 publications

Gut microbiome and plasma metabolome alterations in myopic mice

Gut microbiome and plasma metabolome alterations in myopic mice

Dysfunction of cecal microbiota and CutC activity in mice mediating diarrhea with kidney-yang deficiency syndrome

Renshen Yangrong decoction for secondary malaise and fatigue: network pharmacology and Mendelian randomization study

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