Association of serum podocalyxin levels with peripheral arterial disease in patients with type 2 diabetes

El-Ashmawy, Hazem M.; Selim, Fayrouz Othman; Hosny, Thoraya; Almassry, Hosam Nabil

doi:10.1016/j.jdiacomp.2019.04.003

Cited by 13 publications

(9 citation statements)

References 30 publications

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“…Previous study showed that the re-lease of Podxl from injured podocytes occurs as vesicle-like struc-tures and/or as a result of shedding microvilli (25) .Various types of cells and tissues express Podxl including neurons, lungs, platelets and vascular en-dothelial cells (26) .Our results of high s-Podxl levels in patients with DM might be due to its release from injured endothelium through amechanism, which may be similar to that of podxl release from in-juredpodoctyes.Previous studies have shown the role of Podxl in endo-thelial function and vascular inflammation (27) . Another study showed that, there was an increase in non-specific inflammatory infiltrates within the vessels and CRP level in mu-rine endothelial cells after conditional knock out of the Podxl gene (28) .In our study, we found higher levels of s-Podxl in patients with DM com-pared with healthy group that s-Podxl might be associ-ated with CVD independently of diabetes (29) .…”

Section: Resultssupporting

confidence: 66%

“…A recent study reported an association between s-Podxl concentrations and markers of CVD.Shoji et.al claimed that s-Podxl levels were significantly associated with cIMT and this association remained significant even after controlling the common CVD risk factors such as diabetes, dyslipidemia, hypertension, sex, age and current smoker (23) . In other study illustrated, the number of cases with diabetic and CHD increased with increasing tertiles of s-Podxl from 11(15%) in the 1st tertile, over 22 (30%) in the 2nd tertile, to 25 (33%) in the 3rd tertile (24) .…”

Section: Resultsmentioning

confidence: 68%

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Serum Podocalyxin Levels as High Risk Markers for CVDs of Complication of Diabetes

Hussein DhaherAyyez Al-fatlawiy,

Hanaa Addai Ali

2021

MLU

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Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The chronic hyperglycemia of diabetes is associated with long-term damage, dysfunction, and failure of various organs, especially the eyes, kidneys, nerves, heart, and blood vessels; Podocalyxin is the major sialoprotein in the glycocalyx of glomerular podocytes, podocalyxin was found in the blood vessels of several organs. Objective: The aim of the present study was to elevate serum podocalyxin and some biochemical parameters in diabetic patients compare with healthy group. Method: The present case-control study included 90 participants divided into two groups: 60 patients with diabetes mellitus (40 males and 20 females; aged 49.87 ±8.089 yr.) study group and 30 (20 males and 10 females; aged 48.27±6.335 yr.) a healthy group (control group). Podocalyxin (PODXL) and insulin measured using the ELISA kit. Anthropometric were measured fasting serum glucose levels and lipid profile were determined by photometric method. Result: The mean serum podocalyxin levels was significantly increase in patients with diabetes mellitus Vs. healthy control group (26.24±2.11ng\ml Vs. 17.45±1.57ng\ml respectively, p<0.001), BMI was significantly increased in patients diabetes mellitus Vs. healthy control group (29.766±3.225 Vs. 23.756±0.77 respectively, p<0.001) also the mean fasting insulin level was higher in patients with diabetes mellitus than the healthy control group (7.23±1.26 Vs. 5.05±1.14 respectively, p<0.001), HOMA-IR significantly increased in patients with diabetes mellitus Vs. healthy control group (2.65±0.77 Vs. 1.16±0.25 respectively, p<0.001) but the mean of HOMA-β in patients with diabetes mellitus was significantly lower than the healthy control group (35.30±13.83 Vs. 61.91±19.87 respectively, p<0.001), lipid profile include TG, TC, LDL-C, VLDL-C significant increased in patients with diabetes mellitus compared with healthy control group (211.50±88.02 Vs. 116.87±38.43, p<001), (240.13±56.20 Vs. 141.43±33.34, p<0.001), (161.87±56.39 Vs. 65.82±30.97, p<0.001), (42.30±17.60 Vs. 23.37±7.68, p<0.001) respectively except HDL-C were significantly decreased in patients with diabetes mellitus than the healthy control group (33.83±9.02 Vs. 52.23±7.08 respectively, p<0.001), Patients with diabetic mellitus have significantly higher serum podocalyxin levels than the controls. Podocalyxin levels positively significantly correlated with BMI, FSG, Insulin, HbA1c and HOMA-IR levels while HOMO-β significant negative correlation respectively with podocalyxin levels. podocalyxin may be a promising biomarker in patients with diabetes mellitus. Conclusion: Podocalyxin may be a promising biomarker in patients with diabetes mellitus and can be used as a high risk markers for CVDs in DM patients.

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 68%

Serum Podocalyxin Levels as High Risk Markers for CVDs of Complication of Diabetes

Hussein DhaherAyyez Al-fatlawiy,

Hanaa Addai Ali

2021

MLU

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“…A reported loss-of-function mutation in PODXL has been reported clinically with a very similar phenotype to the one observed in PODXL knockout mice [ 134 ]. More recently, podocalyxin has been studied outside the kidney for its role in other diseases like cancer, neurologic and atherosclerotic diseases [ 132 , 135 , 136 , 137 ].…”

Section: Structural Regulation Of Actin Dynamics In Podocytesmentioning

confidence: 99%

Regulation of the Actin Cytoskeleton in Podocytes

Blaine

Dylewski

2020

Cells

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Podocytes are an integral part of the glomerular filtration barrier, a structure that prevents filtration of large proteins and macromolecules into the urine. Podocyte function is dependent on actin cytoskeleton regulation within the foot processes, structures that link podocytes to the glomerular basement membrane. Actin cytoskeleton dynamics in podocyte foot processes are complex and regulated by multiple proteins and other factors. There are two key signal integration and structural hubs within foot processes that regulate the actin cytoskeleton: the slit diaphragm and focal adhesions. Both modulate actin filament extension as well as foot process mobility. No matter what the initial cause, the final common pathway of podocyte damage is dysregulation of the actin cytoskeleton leading to foot process retraction and proteinuria. Disruption of the actin cytoskeleton can be due to acquired causes or to genetic mutations in key actin regulatory and signaling proteins. Here, we describe the major structural and signaling components that regulate the actin cytoskeleton in podocytes as well as acquired and genetic causes of actin dysregulation.

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“…In peripheral arterial diseases associated with diabetes mellitus, PODXL is shed from vascular endothelial cells and flows into the bloodstream 34 . This indicates the possibility that PODXL shedding occurs in BMECs due to vascular endothelial damage, which occurs in systemic diseases such as hypertension, diabetes, and dyslipidemia.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of Podocalyxin Post-Transcriptionally Induces the Expression and Activity of ABCB1/MDR1 in Human Brain Microvascular Endothelial Cells

Nagano

Ogata

Ito

et al. 2022

Journal of Pharmaceutical Sciences

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Association of serum podocalyxin levels with peripheral arterial disease in patients with type 2 diabetes

Cited by 13 publications

References 30 publications

Serum Podocalyxin Levels as High Risk Markers for CVDs of Complication of Diabetes

Serum Podocalyxin Levels as High Risk Markers for CVDs of Complication of Diabetes

Regulation of the Actin Cytoskeleton in Podocytes

Knockdown of Podocalyxin Post-Transcriptionally Induces the Expression and Activity of ABCB1/MDR1 in Human Brain Microvascular Endothelial Cells

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