Association of serum arginase I with L-arginine, 3-nitrotyrosine, and exhaled nitric oxide in healthy Japanese workers

Abstract

The associations of serum arginase I with serum L-arginine, serum 3-nitrotyrosine, and fractional exhaled nitric oxide (FENO) were evaluated cross-sectionally in healthy Japanese workers. The serum median (minimum-maximum) levels of arginase I, 3-nitrotyrosine, and FENO in healthy people (n = 130) were 14.6 (0.94-108.1) ng/mL, 81.0 (0.27-298.6) pmol/mg protein, and 14.0 (5.0-110.0) parts per billion, respectively. Significant correlations of arginase I with FENO, L-arginine, 3-nitrotyrosine, and percent predicted forced expiratory volume in 1 s (FEV1 (% predicted)) were observed, and correlations of FENO with immunoglobulin E (IgE), NOx, arginase I, and sex and allergy were also observed. By multiple regression analysis, arginase I showed positive associations with FENO and 3-nitrotyrosine, and a negative association with L-arginine; and FENO showed positive associations with IgE and NO2(-) + NO3(-) (NOx), and a negative association with L-arginine, as well as an association with sex. Moreover, logistic regression analysis showed linear inverse associations of arginase I and 3-nitrotyrosine with L-arginine, and showed linear positive associations of FENO with IgE and NOx. It was concluded that serum arginase I might regulate serum L-arginine and 3-nitrotyrosine via L-arginine, and that IgE or NOx might regulate FENO in a healthy Japanese population.

Association of arginase I or nitric oxide-related factors with job strain in healthy workers

“…Social support was positively associated with arginase I. Previously, we showed a high serum level of arginase I in asthma and the correlation of arginase I with hs-CRP [30], as well as the association of arginase I with oxidative stress [17, 18]. Oxidative stress is associated with depression [31–34].…”

Relationship between serum arginase I and l-arginine or exhaled nitric oxide in asthma

“…No correlation was found between arginase activity and L-arginine in healthy controls or asthmatic patients [14]. We previously demonstrated that arginase I was negatively associated with L-arginine and 3-nitrotyrosine in healthy individuals [15]. Decreases in L-arginine levels by arginase I may contribute to the generation of 3-nitrotyrosine, an oxidative stress biomarker, due to the uncoupling of NOS.…”

“…In a healthy population, we found a relationship between serum arginase I and serum L-arginine [15]. After liver transplantation, an inconsistency was noted in the reduction of L-arginine by the release of arginase [16,17].…”

“…FENO in healthy individuals may be associated with several factors such as age, gender, atopic constitution, smoking, and diet [19,20,21]. In a healthy Japanese population, an inverse association between FENO and L-arginine and a positive association between FENO and arginase I were observed in addition to FENO being associated with IgE and NOx [15]. In asthmatic patients in the present study, a positive association was observed between FENO and arginase I in addition to the inverse association between arginase I and L-arginine.…”

“…In healthy Caucasian adults, several factors such as age, gender, atopic constitution, smoking, and diet have been shown to influence FENO [19,20,21]. In a healthy Japanese population, an inverse association was observed between L-arginine and FENO, while FENO was associated with IgE and NOx [15]. Therefore, it is a prerequisite to evaluate the relationship of elevated levels of arginase I with FENO and L-arginine in asthma.…”