Association of serum and follicular fluid SHBG levels andSHBG(TAAAA)npolymorphism with follicle size in women undergoing ovarian stimulation

Xita

et al. 2011

Purpose The association of cytochrome P450 aromatase gene CYP19(TTTA) n polymorphism with ovarian response to FSH stimulation was explored. Methods Three hundred women undergoing medically assisted reproduction and 300 women with at least one spontaneous pregnancy participated in the study. CYP19(TTTA) n polymorphism was genotyped, while serum hormones were determined. During oocyte retrieval, the follicular size, the follicle and oocyte numbers were recorded. Results Six CYP19(TTTA) n alleles with 7 to 12 repeats were revealed. Women homozygous for long CYP19(TTTA) n alleles presented with lower serum FSH levels at the third day of the menstrual cycle (p<0.001) and higher large follicle numbers (p<0.01), compared to women homozygous for short CYP19(TTTA) n alleles. The CYP19(TTTA) 7 allele was associated with higher serum FSH levels (p< 0.003), with lower total follicle (p<0.02) and large follicle numbers (p<0.03), while CYP19(TTTA) 7 allele-carriers presented more frequently with small follicles than CYP19 (TTTA) 7 allele-non carriers (p<0.01). Conclusions CYP19 genetic variants were associated with ovarian reserve and response to standard gonadotrophin stimulation of women undergoing in vitro fertilization.

Section: Subjectsmentioning

confidence: 99%

Aromatase (CYP19) gene variants influence ovarian response to standard gonadotrophin stimulation

Xita

et al. 2011

“…All women of the study population underwent a long GnRH agonist stimulation protocol as previously described [24]. Conventional IVF was performed in all couples.…”

Section: Subjectsmentioning

confidence: 99%

Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

Vartholomatos

et al. 2011

Purpose Sperm flow cytometry (SFC) was used to evaluate the association of sperm chromatin condensation and ploidy with fertilization, embryo development, pregnancy and abortion rates following IVF. Methods Conventional semen analysis was performed in one hundred fifty men, as well as SFC analysis, after acridine orange and propidium iodide staining, for the evaluation of sperm maturity and ploidy respectively. Conventional IVF was performed in all couples. Results Couples with low percentages of mature spermatozoa presented with lower fertilization rates (p < 0.005), lower rates of grade A embryos (p < 0.003) and lower pregnancy rates (p < 0.006), compared to couples with high percentages of mature spermatozoa. Couples with low total aneuploidy rates presented with higher fertilization rates (p < 0.007), higher rates of grade A embryos (p < 0.004) and higher pregnancy rates (p < 0.003), compared to couples with high total aneuploidy rates. Conclusions Sperm chromatin condensation and ploidy constitute critical parameters for the evaluation of semen samples before IVF and for the identification of cases in need of ICSI application.

“…Previous studies of our group have shown significant associations of FSHR 307(T/A)/FSHR 680(N/S), SHBG (TAAAA) n and CYP19(TTTA) n polymorphisms with the ovarian response to standard gonadotrophin stimulation [11][12][13]. The aim of the current study was to investigate the potential synergistic effect of the above polymorphisms on the follicular numbers and size, the oocyte numbers, the serum hormone levels and the pregnancy rate of women undergoing FSH stimulation for in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), due to tubal or male infertility factor.…”

Section: Introductionmentioning

confidence: 70%

“…All women of the study population underwent a long GnRH agonist stimulation protocol as previously described [11,14]. Oocytes were retrieved by follicle aspiration by the transvaginal route under ultrasound guidance and the follicles were stratified into two groups according to their diameter (small follicles with diameter ≤12 mm and large follicles with diameter ≥18 mm).…”

Section: Methodsmentioning

confidence: 99%

The ovarian response to standard gonadotrophin stimulation depends on FSHR, SHBG and CYP19 gene synergism

Pamporaki

et al. 2012