Association of S100B polymorphisms and serum S100B with risk of ischemic stroke in a Chinese population

Abstract

The levels of serum S100B were elevated in patients with ischemic stroke (IS), which may be a novel biomarker for diagnosing IS. The aim of this study was to investigate the association of S100B polymorphisms and serum S100B with IS risk. We genotyped the S100B polymorphisms rs9722, rs9984765, rs2839356, rs1051169 and rs2186358 in 396 IS patients and 398 controls using polymerase chain reaction-single base extension (SBE-PCR). Serum S100B levels were measured by enzyme-linked immunosorbent assay (ELISA). Rs9722 was associated with an increased risk of IS (AA vs. GG: adjusted OR = 2.172, 95% CI, 1.175–4.014, P = 0.013; dominant: adjusted OR = 1.507, 95% CI, 1.071–2.123, P = 0.019; recessive: adjusted OR = 1.846, 95% CI, 1.025–3.323, P = 0.041; additive: adjusted OR=1.371, 95% CI, 1.109-1.694, P = 0.003). The A-C-C-C-A haplotype was associated with an increased risk of IS (OR = 1.325, 95% CI, 1.035–1.696, P = 0.025). In addition, individuals carrying the rs9722 GA/AA genotypes had a higher serum S100B compared with the rs9722 GG genotype in IS patients (P = 0.018). Our results suggest that the S100B gene rs9722 polymorphism may contribute to the susceptibility of IS, probably by promoting the expression of serum S100B.

A functional polymorphism in the promoter of TUG1 is associated with an increased risk of ischaemic stroke

“…For example, siblings or mothers of stroke case patients had 2‐4 times higher risk of stroke compared to controls . Our previous work identified some susceptibility loci of IS, such as rs9722 AA in S100 calcium‐binding protein B, rs1804826 T in growth differentiation factor‐15 and rs4705342 TT in the promoter of miR‐143/145 …”

S100B single nucleotide polymorphisms exhibit sex-specific associations with chronic pain in sickle cell disease in a largely African-American cohort

“…Another study found that in Han Chinese patients with major depressive disorder (MDD), those carrying the AA genotype of rs9722 had an earlier age of onset than the GA and GG genotypes, and were more vulnerable to recurrent episodes of depression [37]. A similar study in Han Chinese patients with ischemic stroke found that the A allele was associated with increased risk of stroke as well as elevated serum S100B [35].…”

“…SNPs were selected based on extensive literature search. Since S100B polymorphisms have not been previously studied in the context of pain, we included SNPs that have been associated with comorbid neurological disorders in prior studies [9,[35][36][37][38]. Genotyping success rate of SNPs included in this study analyses was >90% and SNPs were in Hardy-Weinberg equilibrium as determined by a χ 2 goodness-of-fit test.…”

A functional variant rs12904 in the miR-200c binding site was associated with a decreased risk of ischemic stroke

“…The study subjects included 328 patients with IS and 331 controls who were collected from the Affiliated Hospital of Youjiang Medical University for Nationalities, Guangxi, China between January 2013 and September 2016. Detailed information of the study population was described in our previous work [9]. Briefly, IS was defined as an acute focal or global neurologic deficit that persisted for more than 24 h. IS diagnosis was confirmed by clinical symptoms, physical examinations and cranial computed tomography or magnetic resonance imaging.…”

“…For example, family history is a risk factor for stroke, and monozygotic twins are more likely to be concordant than dizygotic twins [8]. Our previous work showed that S100B rs9722, growth differentiation factor-15 ( GDF-15 ) rs1804826, and miR-143/145 rs4705342 were genetic risk factors for the occurrence of IS, probably by affecting the expression levels of serum S100B, soluble GDF-15, and miR-145 [9–11].…”