Association of S100A7 gene polymorphisms with manifestations of common types of psoriasis: effect on serum calcium levels

Abstract

ROS deleterious for the cells (Fig. 2d,e). TIEG1 protein expression was assessed at different time points after treatment, and expression levels calculated relative to untreated cells (Fig. 2e). NHF were particularly sensitive to stress induction, with a marked and prolonged decrease of TIEG1 expression shortly after H 2 O 2 , UVA and to a lesser extent with UVB treatment (Fig. 2e).Additional experiments can be found in supplementary data. ConclusionTIEG1 is a mediator of TGF-b pathway with an important function in bone tissue regulation (4). Here, we thoroughly described for the first time TIEG1 expression and regulation in human skin cells. We found that TIEG1 was well expressed in NHF and NHK at the mRNA and at the protein level both in primary cultures and in human skin biopsies. The function of TIEG1 as a TGF-b mediator was also revealed in human skin cells and therefore conserved from bone to skin tissues. TIEG1 expression was decreased after exogenous stress such as UV irradiation or H 2 O 2 treatment on primary cell cultures of NHF and NHK, and we found a correlation between in vivo skin photoexposure and low TIEG1 expression. Because TIEG1 is a positive mediator of TGF-b signalling, our results indicate that photoexposure lowers responsiveness to TGF-b pathway by decreasing TIEG1 expression. Previous reports suggest that UV irradiation blocks cellular responses to TGF-b primarily by downregulating its receptor expression (5,8). Here, we describe another mechanism by which stress can affect this multifunctional cytokine that regulates cell growth and differentiation.Our work suggests that TIEG1 might play an essential role for human skin both in dermis and epidermis although functional studies need to be performed in order to support this hypothesis. Acknowledgements Conflict of interestsNone. Supporting InformationAdditional supporting data may be found in the supplementary information of this article.Data S1. Materials and methods. Data S2. Results and discussion. Figure S1. Controls for TIEG1 protein detection. Figure S2. TIEG1 is induced by TGF-b in human skin cells. Figure S3. TIEG1 expression is not correlated to aging.

Low vitamin D-modulated calcium-regulating proteins in psoriasis vulgaris plaques: S100A7 overexpression depends on joint involvement

“…S100A7 has been shown to enhance osteoclast formation in vitro ( 82 ). Moreover, a S100A7 single nucleotide polymorphism has been shown to be associated with psoriatic arthritis ( 83 ).…”