Association of porous hydroxyapatite and bone marrow cells for bone regeneration

Anselme, Karine; Noël, Benoît; Flautre, B.; Blary, M. C.; Delecourt, C.; Descamps, M.; Hardouin, Pierre

doi:10.1016/s8756-3282(99)00133-7

Cited by 73 publications

(53 citation statements)

References 9 publications

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“…In recent years, synthetic and natural porous ceramic materials have been widely used as scaffolds in bone tissue engineering (Anselme et al, 1999;Hench and Polak, 2002;Cancedda et al, 2003). Major requirements for such materials are biocompatibility, porosity and a biodegradation rate corresponding to the one of bone formation upon replacement of the scaffold by the new bone tissue (Langer and Vacanti, 1993).…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of porous calcium phosphate scaffolds in an ectopic bone formation model studied by X-ray computed microtomograph

Komlev

Mastrogiacomo²,

Pereira

et al. 2010

eCM

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Three types of ceramic scaffolds with different composition and structure [namely synthetic 100% hydroxyapatite (HA; Engipore), synthetic calcium phosphate multiphase biomaterial containing 67% silicon stabilized tricalcium phosphate (Si-TCP; Skelite™) and natural bone mineral derived scaffolds (Bio-oss®)] were seeded with mesenchymal stem cells (MSC) and ectopically implanted for 8 and 16 weeks in immunodeficient mice. X-ray synchrotron radiation microtomography was used to derive 3D structural information on the same scaffolds both before and after implantation. Meaningful images and morphometric parameters such as scaffold and bone volume fraction, mean thickness and thickness distribution of the different phases as a function of the implantation time, were obtained. The used imaging algorithms allowed a direct comparison and registration of the 3D structure before and after implantation of the same sub-volume of a given scaffold. In this way it was possible to directly monitor the tissue engineered bone growth and the complete or partial degradation of the scaffold. Further, the detailed kinetics studies on Skelite™ scaffolds implanted for different length of times from 3 days to 24 weeks, revealed in the X-ray absorption histograms two separate peaks associated to HA and TCP. It was therefore possible to observe that the progressive degradation of the Skelite™ scaffolds was mainly due to the resorption of TCP. The different saturation times in the tissue engineered bone growth and in the TCP resorption confirmed that the bone growth was not limited the scaffold regions that were resorbed but continued in the inward direction with respect to the pore surface.

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Section: Introductionmentioning

confidence: 99%

Biodegradation of porous calcium phosphate scaffolds in an ectopic bone formation model studied by X-ray computed microtomograph

Komlev

Mastrogiacomo²,

Pereira

et al. 2010

eCM

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“…Since the early nineties, many investigators have demonstrated the concept of combining osteoprogenitor cells with an appropriate scaffold to be osteogenic ectopically in rodents and larger mammals. [1][2][3][4] Recently, ectopic tissue-engineered bone formation in a goat model has been demonstrated. 5,6 Although less informative with respect to clinical application, the advantage of an ectopic model is that it allows fundamental research without the disturbing influence of host bone as present orthotopically.…”

Section: Introductionmentioning

confidence: 99%

Optimization of bone‐tissue engineering in goats

et al. 2004

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Successful bone-tissue engineering (TE) has been reported for various strategies to combine cells with a porous scaffold. In particular, the period after seeding until implantation of the constructs may vary between hours and several weeks. Differences between these strategies can be reduced to (a) the presence of extracellular matrix, (b) the differentiation status of the cells, and (c) the presence of residual potentially immunogenic serum proteins. These parameters are investigated in two types of calcium phosphate scaffolds in a goat model of ectopic bone formation. Culture-expanded bone-marrow stromal cells from eight goats were seeded onto two types of hydroxyapatite granules: HA60/400 (60% porosity, 400-microm average pore size) and HA70/800. Scaffolds seeded with cells and control scaffolds were cultured for 6 days in medium containing autologous or semisynthetic serum, in the presence or absence of dexamethasone. Other scaffolds were seeded with cells just before implantation in medium with or without serum. All conditions were implanted autologously in the paraspinal muscles. After 12 weeks, bone had formed in 87% of all TE constructs, as demonstrated by histology. Histomorphometry indicated significantly more bone in the HA70/800 scaffolds. Furthermore, a significant advantage in bone formation was found when the constructs had been cultured for 6 days. In conclusion, both scaffold characteristics (porosity) and TE strategy (culturing of the constructs) were demonstrated to be important for bone TE.

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“…Obviously, this is a very positive result also obtained with other cell types as well, e.g. (Anselme et al 1999;Stevens et al 2005). The use of cells for tissue engineering comes with a drawback.…”

Section: Foetal Cell Therapymentioning

confidence: 65%

Integration of mechanotransduction concepts in bone tissue engineering

Pioletti

2013

Computer Methods in Biomechanics and Biomedical Engineering

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Mechanical stimulus has been identified for a long time as a key player in the adaptation of the musculo-skeletal tissues to their function. Mechanical loading is then an intrinsic variable to be considered when new developments are proposed in bone tissue engineering. By combining structural biomechanics and mechanotransduction aspects, a new paradigm is presented for bone tissue engineering. It is proposed that in vivo mechanical loading be used to increased bone formation in the scaffold instead of pre-seeding the scaffold with cells or delivering growth factors. In this article, we demonstrated the feasibility of this approach and compared it to the classical tissue engineering strategy. In particular, we showed that bone formation could be increased in the scaffold that underwent mechanical loading during an in vivo study in rats. A model of bone formation was then proposed to translate the in vivo results into a possible clinical application where the loading of the scaffold would be transmitted by the sharing of the load between an implant and the bone scaffold.

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Association of porous hydroxyapatite and bone marrow cells for bone regeneration

Cited by 73 publications

References 9 publications

Biodegradation of porous calcium phosphate scaffolds in an ectopic bone formation model studied by X-ray computed microtomograph

Biodegradation of porous calcium phosphate scaffolds in an ectopic bone formation model studied by X-ray computed microtomograph

Optimization of bone‐tissue engineering in goats

Integration of mechanotransduction concepts in bone tissue engineering

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