Association of polymorphisms of vasoactive intestinal peptide and its receptor with reproductive traits of turkey hens

Abstract

The aim of this study was to identify variations of vasoactive intestinal peptide (VIP) and VIP receptor-1 (VIPR-1) genes that might be associated with turkey reproductive traits. One hundred twenty turkey hens were recorded for age at first egg (AFE), first egg weight (FEW), egg number (EN), total egg weight (TEW), laying period (LP), and broodiness. The DNA was isolated from blood samples and subjected to PCR amplification of the meleagrine VIP and VIPR-1 genes. The SNPs were detected by single-strand conformation polymorphism and the variant DNA fragments were sequenced. One mutation in 3’-UTR of VIP (G5846A) and two SNPs in intron 2 of VIPR-1 (C17687T and A17690T) were found, all of them novel. The associations of the three detected SNPs with the reproductive traits of turkeys were evaluated. The detected polymorphisms were used for marker-trait association analyses. The results of association analysis showed that G5846A on 3’ UTR of VIP has a significant association with LP, EN, TEW, and AFE. The G allele of G5846A was the favourable SNP allele for LP, EN, and TEW traits. The AA genotype of A17690T on intron 2 of VIPR-1 was significantly associated with higher LP, EN, and TEW. AGAA haplotype showed association with higher EN and TEW. These results suggest that the SNPs in 3’-UTR of VIP and intron 2 of VIPR-1 genes may influence egg production traits in turkey hens.Keywords: broodiness, candidate gene, egg production, single nucleotide polymorphism, VIPR-1

Research Note: Association of VIPR-1 gene polymorphism with growth traits in meat type Japanese quail (Coturnix Japonica)

“… Zhou et al (2008) have shown that 7 sites (A-284G, A+457G, C+598T, D+19820I, C+37454T, C+42913T, and C+53327T) of VIPR-1 gene might be associated with broodiness. Hosseinpour et al (2020) have shown that 2 SNPs (C17687T and A17690T) of VIPR-1 gene were associated with egg production traits, egg number, total egg weight, and laying period in turkey hens. The present study was conducted to determine if the polymorphisms of the VIPR-1 gene were correlated with the growth traits at 3 and 5 wk of age in quails ( Figure 4 ).…”

Non-synonymous variation and protein structure of candidate genes associated with selection in farm and wild populations of turbot (Scophthalmus maximus)

“…VIP binds to the N-terminal end of the receptor, which in turbot contains an Asn2Gln polymorphism. VIPR1 polymorphism has been linked to gastrointestinal dysmotility disorders in man 120 , but associated with reproductive traits in birds 121 . Two polymorphic hepcidins have been identified in turbot 122 of which hep1 was highly polymorphic in all populations and farms examined, particularly in the Black Sea and farm 3.…”