Association of polymorphisms in <scp>NR</scp>6A1,<scp>PLAG</scp>1 and <scp>VRTN</scp> with the number of vertebrae in Chinese Tongcheng × Large White crossbred pigs

Lan

et al. 2020

Simple Summary: An increase in the number of ribs (RIB) could improve carcass length (CL) and body size. Cannon bone circumference (CBC) is a pivotal body size trait, and a large CBC could enhance the capacity to bear excessive body weight, vigorous exercise, and resistance to injuries. Several researchers showed that the vertnin (VRTN) gene g.20311_20312ins291 (NC_010449.5 7: g.20311_20312ins291) is an important variant that is related to RIB and CL of Western pigs. However, it is unknown whether this variant could affect the CBC of pigs. Our study showed that this variant was significantly associated with RIB, carcass diagonal length (CDL), and CBC in Suhuai pigs; therefore, it could be used as a potential molecular marker for improving RIB, CDL, and CBC in this breed. Abstract:The vertnin (VRTN) gene g.20311_20312ins291 was reported as an important variant related to the number of ribs (RIB), and the ins/ins genotype was advantageous for improving RIB of Western pigs. The purpose of this study was to determine whether the VRTN gene g.20311_20312ins291 influences RIB, carcass traits, and body size traits, including cannon bone circumference (CBC) in Chinese Suhuai pigs. We found that the VRTN gene g.20311_20312ins291 was polymorphic in Suhuai fattening pigs and gilts. The polymorphism of g.20311_20312ins291 was significantly associated with RIB and CDL in Suhuai fattening pigs (p < 0.01), whereas this variant had no influence on carcass weight (CWT). There was a tendency of association between this variant and carcass straight length (CSL) in Suhuai fattening pigs (p = 0.06). The polymorphism of g.20311_20312ins291 was also significantly associated with CBC in Suhuai gilts (p = 0.04). Furthermore, CBC was positively genetically correlated with body length (0.22, p < 0.01) and body weight (0.15, p < 0.01). Our results indicated that the VRTN gene g.20311_20312ins291 could be used as a potential marker for improving RIB, CDL, and CBC in Suhuai pigs.

Section: Discussionsupporting

confidence: 81%

Polymorphism of VRTN Gene g.20311_20312ins291 Was Associated with the Number of Ribs, Carcass Diagonal Length and Cannon Bone Circumference in Suhuai Pigs

Jiang

Lan

et al. 2020

“…Furthermore, polymorphisms in the NR6A1 gene in Duroc pigs may affect body length [15]. It was confirmed that NR6A1 is prominently associated with the number of vertebrae in Tongcheng (TC) pigs [16]. Similar results were found in Licha Black and Laiwu pigs [17].…”

Section: Introductionmentioning

confidence: 72%

“…In addition, the high homology of NR6A1 among different species indicates its potential functional conservation [18]. Based on previous research [8,9,10,11,12,13,14,15,16,17,18], it is rational to hypothesize that the NR6A1 gene may be associated with body size traits of other species. Presently, there is no research regarding the NR6A1 gene in donkeys.…”

Section: Introductionmentioning

confidence: 99%

A Novel 13 bp Deletion within the NR6A1 Gene Is Significantly Associated with Growth Traits in Donkeys

Fang

Lai

et al. 2019

Simple SummaryThe detection of genes potentially associated with economic traits and identification of effective variants can provide a basis for molecular marker-assisted selection of livestock. NR6A1 is a member of the nuclear receptor family and is an important candidate gene related to body size traits. Previous studies showed that NR6A1 gene was associated with body size traits in pigs and other livestock, however, it has not yet been observed in donkeys. In the current study, a 13 bp deletion in NR6A1 gene was firstly identified in donkeys. Analysis showed that this deletion had significant associations with body size traits.AbstractNuclear receptor subfamily 6, group A, member 1 (NR6A1), as an important member of the nuclear receptor family, plays an important role in regulating growth, metabolism, and differentiation of embryonic stem cells. For this reason, the NR6A1 gene is considered to be a promising candidate for economic traits and was found to be associated with body size traits in many livestock. However, no studies have been conducted on NR6A1 in donkeys so far. Thus, in this research, we focused on donkeys and identified a 13 bp deletion in intron-1 of the NR6A1 gene among 408 individuals from Guanzhong and Dezhou donkeys using polyacrylamide gel electrophoresis. Three genotypes were identified, namely II, ID, and DD. The association analysis indicated that the body lengths and body heights5f genotype II individuals were significantly different to those of genotype ID in Dezhou donkeys. Conclusively, the 13 bp deletion was associated with growth traits in both Guanzhong donkeys and Dezhou donkeys, indicating that the NR6A1 gene could be a possible candidate gene in marker-assisted selection for donkey breeding programs.

“…The NTV in pigs is a highly heritable trait (heritability from 0.24 to 0.78) and shows considerable variation across breeds [ 3 , 5 ]. Many studies have shown that the NTV is a polygenic trait influenced by many different quantitative trait loci (QTL) on Sus scrofa Chromosomes (SSCs) 1, 2, 4, 7, 11, 16, and 18 [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Genome-wide association studies (GWASs) in recent years have shown that the major genes controlling the NTV in pigs are concentrated on SSC7 [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Analysis Identified BMPR1A as a Novel Candidate Gene Affecting the Number of Thoracic Vertebrae in a Large White × Minzhu Intercross Pig Population

Yue

Niu

et al. 2020

The number of vertebrae (NV), especially the number of thoracic vertebrae (NTV), varies among pig breeds. The NTV is controlled by vertebral segmentation and the number of somites during embryonic development. Although there is a high correlation between the NTV and NV, studies on a fixed NV have mainly considered the absolute numbers of thoracic vertebrae instead of vertebral segmentation. Therefore, this study aimed to discover variants associated with the NTV by considering the effect of the NV in pigs. The NTV and NV of 542 F2 individuals from a Large White × Minzhu pig crossbreed were recorded. All animals were genotyped for VRTN g.19034 A > C, LTBP2 c.4481A > C, and 37 missense or splice variants previously reported in a 951-kb interval on SSC7 and 147 single nucleotide polymorphisms (SNPs) on SSC14. To identify NTV-associated SNPs, we firstly performed a genome-wide association study (GWAS) using the Q + K (population structure + kinship matrix) model in TASSEL. With the NV as a covariate, the obtained data were used to identify the SNPs with the most significant genome-wide association with the NTV by performing a GWAS on a PorcineSNP60K Genotyping BeadChip. Finally, a conditional GWAS was performed by fixing this SNP. The GWAS showed that 31 SNPs on SSC7 have significant genome-wide associations with the NTV. No missense or splice variants were found to be associated with the NTV significantly. A linkage disequilibrium analysis suggested the existence of quantitative trait loci (QTL) in a 479-Kb region on SSC7, which contained a critical candidate gene FOS for the NTV in pigs. Subsequently, a conditional GWAS was performed by fixing M1GA0010658, the most significant of these SNPs. Two SNPs in BMPR1A were found to have significant genome-wide associations and a significant dominant effect. The leading SNP, S14_87859370, accounted for 3.86% of the phenotypic variance. Our study uncovered that regulation variants in FOS on SSC7 and in BMPR1A on SSC14 might play important roles in controlling the NTV, and thus these genetic factors may be harnessed for increasing the NTV in pigs.