Association of Polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep

Moradband, F.; Rahimi, G.; Gholizadeh, Mohsen

doi:10.5713/ajas.2011.10453

Cited by 48 publications

(40 citation statements)

References 20 publications

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“…Identifying genes of major effect is also a prerequisite which offers the opportunity to improve production efficiency, product quality and product diversity in livestock industry, through utilizing them in breeding programs [1]. Most mutations in proteins are associated with diseases; only a small number of examples show the heterozygote advantage.…”

Section: Introductionmentioning

confidence: 99%

Computational Molecular Analysis of the Sequences of BMP15 Gene of Ruminants and Non-Ruminants

Bibinu¹,

Yakubu²,

Ugbo³

et al. 2016

OJGen

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Bone morphogenetic protein 15 (BMP15) is a member of the transforming growth factor β (TGFβ) super family that is expressed by oocytes and plays key roles in granulosa cell development and fertility in animal. This study investigated the molecular genetic variation of BMP15 gene of some selected mammalian species with a view to providing relevant genetic information for breeding and selection programmes in the studied species using computational methods. A total of thirty seven (37) BMP15 nucleotide sequences comprising goats (18), sheep (6), cattle (6), swine (4) and chicken (3) were retrieved from the GenBank. Sequence alignment, translation and comparison of the BMP15 gene of the various species were done with ClustalW. High degree of polymorphism of BMP15 gene was observed among the studied species. The significant value (P < 0.01) for relative proportions of non-synonymous substitutions per non-synonymous site (dN) and the number of synonymous substitutions per synonymous site (dS) symbolized that non-synonymous sites evolved faster than the synonymous sites and positive selection effect over shadowed purifying selection. Functional analysis of missense mutations using PROVEAN showed that twelve amino acid substitutions (L10S, W13A, E20L, V28S, P31R, P31G, P40Q, L42W, Q46N, A52V, R58C and G64T) in goats, nine in sheep (H21R, S32R, I33A, A39W, Q46W, E51A, G54S, R61D and E72A), six in cattle (Q30M, T41W, E50R, I62R, H65E, and E72S), seven in swine (I7L, T9I, V33I, L35H, C40P, R46P and Q61R) and five in chickens (A20H, L27H, W43L, A47P and G50Y) appeared beneficial. The phylogenetic trees from nucleotide and amino acid sequences revealed the close relatedness of members of the bovidae family (goat, sheep and cattle). The present information could guide future efforts involving * Corresponding authors. B. S. Bibinu et al. 40selection of markers of fecundity to improve genetically livestock species in Nigeria.

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Section: Introductionmentioning

confidence: 99%

Computational Molecular Analysis of the Sequences of BMP15 Gene of Ruminants and Non-Ruminants

Bibinu¹,

Yakubu²,

Ugbo³

et al. 2016

OJGen

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“…The analysis of polymorphism for GDF9 (G1) in Baluchi sheep indicated all three possible genotypes, however allele G had the highest frequency (0.82), whereas allele A had the lowest frequency (0.18) [16]. The genotype frequencies of GG, AG and AA were 0.72.0, 20.0 and 8.0%, respectively.…”

Section: Resultsmentioning

confidence: 98%

Polymorphism of the GDF9 Gene in Russian Sheep Breeds

LV¹

2015

J Cytol Histol

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Growth differentiation factor 9 (GDF9) plays a key role in the fertility of most mammalian species. Ovine GDF9 gene is localized in the 5th chromosome. The gene's length is 2.5 kb consisting of two exons separated by one intron (1126 bp) and encodes a pro-peptide comprising 453 amino acids. The mature peptide includes 135 amino acids.The purpose of this paper is to determine the GDF9 polymorphism in sheep of Salskaya and Romanov breeds in Rostov region of Russia. Polymorphism was identified by PCR-RFLP method at points G1 (G260A) and G4 (G721A). AG and GG genotypes were detected at points G1 and AA and AG genotypes were detected at points G4 in Salskaya sheep breed, frequency of A was 0.05 and G allele 0.95 (at point G1) and frequency of A was 0.95 and G allele 0.05 (at point G4). In Romanov breed AG and GG genotypes were detected at points G1 and AA and AG genotypes were detected at points G4, frequency of A was 0.20 and G allele 0.80 (at point G1) and frequency of A was 0.80 and G allele 0.20 (at point G4).

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“…The FecX G mutation was analyzed in Garole, Kendrapada [12] , Malpura, Deccani, Baluchi [32] and Sangsari sheep [14] ; however, none of these sheep breeds carried the FecX G mutation in the BMP15 gene. Additionally, the FecX G mutation was identified in Small Tailed Han, Belclare and Cambridge sheep [1,33] .…”

Section: Discussionmentioning

confidence: 99%

Sakız Irkı Koyunlarda FecB, BMP15 ve CAST Genlerindeki Mutasyonların Analizi

Dinçel¹,

Ardıçlı²,

Soyudal³

et al. 2015

Kafkas Univ Vet Fak Derg

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Fertility traits, such as the ovulation rate and the number of offspring at birth, are genetically regulated by fecundity genes. This study was performed to identify DNA polymorphisms in Booroola (FecB), Galway (FecX G ), Inverdale (FecX I ) and Calpastatin (CAST) genes in Sakiz sheep. A total of 71 ewes were genotyped for gene or allelic polymorphisms in the genes listed above using the PCR-RFLP method. The results obtained from this study indicated that all of the Sakiz ewes sampled were non-carriers for FecB, FecX G or FecX I mutations. However, genotypic frequencies in the CAST gene were 0.59, 0.36 and 0.05 for AA, AB and BB, respectively. A significant deviation from Hardy-Weinberg equilibrium for the CAST gene was not observed in the investigated breed (P>0.05). As a result, more extensive screening is required as tests for newly discovered mutations are developed. Additionally, this study is the first to report a genetic polymorphism in the CAST gene in Sakiz sheep.

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Association of Polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep

Cited by 48 publications

References 20 publications

Computational Molecular Analysis of the Sequences of BMP15 Gene of Ruminants and Non-Ruminants

Computational Molecular Analysis of the Sequences of BMP15 Gene of Ruminants and Non-Ruminants

Polymorphism of the GDF9 Gene in Russian Sheep Breeds

Sakız Irkı Koyunlarda FecB, BMP15 ve CAST Genlerindeki Mutasyonların Analizi

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