Association of platelet-derived growth factor-D gene polymorphism with ischemic stroke in a Chinese case–control study

Abstract

Stroke is a multiple genetic disease. Platelet-derived growth factor-D has been found to be involved in the pathogenesis of atherosclerosis, suggesting possible association between platelet-derived growth factor-D and the development of ischemic stroke. However, little information on the relationship between platelet-derived growth factor-D and stroke is currently available. The aim of this study was to investigate the association between platelet-derived growth factor-D genetic variation and the risk of ischemic stroke in a Chinese population. We conducted a case-control study with 309 ischemic stroke patients and 309 sex and age (<5 years)-matched controls. DNA was extracted from the whole blood of each participant. Platelet-derived growth factor-D C/G polymorphism at position +3166 (rs7950273) was detected by TaqMan SNP genotyping assay. Overall, the combined rates of platelet-derived growth factor- D CG and GG are 51% in patients in contrast with 46% in controls. There were no significant differences in the genotype frequencies of platelet-derived growth factor-D +3166 polymorphisms between the patients and controls with history or family history of hypertension or diabetes (P = 0.770). However, among people without history or family history of hypertension or diabetes, platelet-derived growth factor-D CG/GG is significantly more frequently expressed in patients (60%) than in controls (43%) (odds ratio 1.97; 95% confidence interval 1.19-3.26). This significant association holds after adjustment for age, sex, smoking and alcohol intaking (odds ratio 1.86; 95% confidence interval 1.11-3.10) (P = 0.018). Our study found that the G allele of rs7950273 of the platelet-derived growth factor-D gene is associated with higher risk of ischemic stroke in a Chinese population without history or family history of hypertension or diabetes. Future studies with larger and ethnically diverse populations are needed to further evaluate the platelet-derived growth factor-D polymorphism and stroke association, as well as its pathophysiological mechanisms.

A functional variant in promoter region of platelet-derived growth factor-D is probably associated with intracerebral hemorrhage

“…However, the associations of PDGFD variants with other type of stroke, especially ICH have not been investigated [18]. In the present study, we found that the -858A/C variation in the PDGFD promoter region could lead to an enhancement of PDGFD promoter activity and consequently up-regulation of the expression of the gene.…”

Molecular mechanisms for the prevention and promoting the recovery from ischemic stroke by nutraceutical laminarin: A comparative transcriptomic approach

“…A PDGFD transgenic mouse study showed that PDGFD could induce blood vessel maturation during angiogenesis (42). More importantly, a case-control study demonstrated an association between PDGFD gene polymorphisms and ischemic stroke (43). Heparin-binding EGF-like growth factor (HBEGF), a hypoxia-inducible neuroprotective protein, was reported to play a neuroprotective role by stimulating the proliferation of neuronal precursor cells during ischemia and reperfusion injury (44).…”