Association of PARP1-specific polymorphisms and haplotypes with non-small cell lung cancer subtypes

Abstract

Objective The carcinogenesis role of PARP1 in lung cancer is still not clear. Analysis at allelic levels cannot fully explain the function of PARP1 on lung cancer. Our study aims to further explore the relation between PARP1 haplotypes and lung cancer. Materials and methods DNA and RNA were extracted from non-small cell lung cancer (NSCLC) tumor and adjacent normal fresh frozen tissue. Five PARP1-SNPs were genotyped and PARP1-specific SNPs were imputed using IMPUTE and SHAPEIT software. The SNPs were subjected to allelic, haplotype and SNP-SNP interaction analyses. Correlation between SNPs and mRNA/protein expressions were performed. Results SNP imputation inferred the ungenotyped SNPs and increased the power for association analysis. Tumor tissue samples are more likely to carry rs1805414 (OR = 1.85; 95% CI: 1.12–3.06; P-value: 0.017) and rs1805404 (OR = 2.74; 95%CI 1.19–6.32; P-value: 0.015) compared to normal tissues. Our study is the first study to show that haplotypes comprising of 5 SNPs on PARP1 (rs1136410, rs3219073, rs1805414, rs1805404, rs1805415) is able to differentiate the NSCLC tumor from normal tissues. Interaction between rs3219073, rs1805415, and rs1805414 were significantly associated with the NSCLC tumor with OR ranging from 3.61–6.75; 95%CI from 1.82 to 19.9; P-value<0.001. Conclusion PARP1 haplotypes may serve as a better predictor in lung cancer development and prognosis compared to single alleles.

Circ_0001944 Contributes to Glycolysis and Tumor Growth by Upregulating NFAT5 Through Acting as a Decoy for miR-142-5p in Non-Small Cell Lung Cancer

“… 1 For early NSCLC patients, their 5-year survival rate can reach about 56% after treatment. 2 However, a large segment of NSCLC patients are frequently diagnosed at an advanced stage and have a 5-year survival rate as low as about 4%. 3 , 4 Hence, understanding the molecular mechanisms that accelerate the advancement of NSCLC is crucial for the development of effective treatment strategies.…”

Insights into the pathogenesis of hereditary angioedema using genetic sequencing and recombinant protein expression analyses

“…The quantity of each recombinant C1-INH variant protein was determined using a C1-INH ELISA kit (Abcam, Cambridge, Mass) asper the manufacturer’s protocol. 22 Purified C1-INH, recombinant C1-INH, and L349F were mixed with biotinylated C1s and then applied to the streptavidin-coated plate. Active C1-INH molecules were detected through binding with biotinylated C1s.…”

Assessing effectiveness of many-objective evolutionary algorithms for selection of tag SNPs

“…Olivia et al [ 15 ] investigated variants that explain brown eye color formation in individuals with the rs12913832:GG genotype. Jing et al [ 16 ] investigated the relation between PARP1 haplotypes and lung cancer. They used SNP imputation in their research.…”