Association of oxidative stress with the formation of reproductive toxicity from mercury exposure on hermaphrodite nematode Caenorhabditis elegans

Wu, Qiuli; He, Ke-Wen; Liu, Peidang; Li, Yinxia; Wang, Dayong

doi:10.1016/j.etap.2011.04.009

Cited by 49 publications

(17 citation statements)

References 35 publications

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“…In C. elegans , toxicants usually cause damage on animals by inducing oxidative stress [18], [29], [53]. We further examined the intestinal ROS production in clentuberol or ractopamine exposed nematodes.…”

Section: Resultsmentioning

confidence: 99%

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Zhuang

Zhao

et al. 2014

PLoS ONE

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In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intestinal autofluorescence and reactive oxygen species (ROS) production. Although acute exposure to the examined concentrations of clentuberol or ractopamine did not induce lethality, prolonged exposure to 10 µg/L of clentuberol and ractopamine reduced lifespan. At relatively high concentrations, ractopamine exhibited more severe toxicity than clentuberol on nematodes. Overexpression of sod-2 gene encoding a Mn-SOD to prevent induction of oxidative stress effectively inhibited toxicity from clentuberol or ractopamine. Besides oxidative stress, we found that clentuberol might reduce lifespan through influencing insulin/IGF signaling pathway; however, ractopamine might reduce lifespan through affecting both insulin/IGF signaling pathway and TOR signaling pathway. Ractopamine more severely decreased expression levels of daf-16, sgk-1, skn-1, and aak-2 genes than clentuberol, and increased expression levels of daf-2 and age-1 genes at the examined concentration. Therefore, the C. elegans assay system may be useful for assessing the possible toxicity from weight loss agents, and clentuberol and ractopamine may induce toxicity through different molecular mechanisms.

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Section: Resultsmentioning

confidence: 99%

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Zhuang

Zhao

et al. 2014

PLoS ONE

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“…The method for ROS production was performed as described previously (Wu et al, 2011). To examine the intestinal ROS production, the examined nematodes were transferred to 1 μM of 5′,6′-chloromethyl-2′,7′dichlorodihydro-fluorescein diacetate (CM-H 2 DCFDA; Molecular Probes) in 12-well sterile tissue culture plates to pre-incubate for 3-h at 20°C in the dark.…”

Section: Intestinal Autofluorescence and Intestinal Ros Productionmentioning

confidence: 99%

Adverse effects of coal combustion related fine particulate matter (PM 2.5 ) on nematode Caenorhabditis elegans

Sun

Liu²,

Liao

et al. 2015

Science of The Total Environment

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“…C. elegans can be used for safety assessment of many toxicants such as heavy metal, drug, and nanomaterials [11]–[14]. The facts that genome and important signal pathways are conserved between C. elegans and human and aging process of C. elegans is similar to human make it an ideal model for aging research and anti-aging drug discovery [15]–[16].…”

Section: Introductionmentioning

confidence: 99%

Beneficial Effects of Wheat Gluten Hydrolysate to Extend Lifespan and Induce Stress Resistance in Nematode Caenorhabditis elegans

Zhang

et al. 2013

PLoS ONE

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Previous studies have showed that wheat gluten hydrolysate (WGH) has the anti-oxidative property. In the present study, we examined the possible safety property of WGH and the beneficial effects of WGH to extend lifespan and induce stress resistance using nematode Caenorhabditis elegans as the in vivo assay system. We found that WGH at concentrations of 0.1–1 mg/mL did not cause lethality, influence development, alter locomotion behavior and brood size, and induce significant intestinal autofluorescence and reactive oxygen species (ROS) production in young adults. Treatment with 0.1–1 mg/mL of WGH significantly extended lifespans of nematodes under the normal conditions. Moreover, WGH treatment significantly inhibited the induction of intestinal autofluorescence and suppressed the decrease in locomotion behavior during the aging process of nematodes. Furthermore, pre-treatment with 1 mg/mL of WGH significantly suppressed the adverse effects caused by heat-stress or oxidative stress on nematodes as indicated by the alterations of both lifespan and intestinal ROS production. Therefore, WGH treatment is relatively safe and has beneficial effects on nematodes under both the normal conditions and the stress conditions.

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Association of oxidative stress with the formation of reproductive toxicity from mercury exposure on hermaphrodite nematode Caenorhabditis elegans

Cited by 49 publications

References 35 publications

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Adverse effects of coal combustion related fine particulate matter (PM 2.5 ) on nematode Caenorhabditis elegans

Beneficial Effects of Wheat Gluten Hydrolysate to Extend Lifespan and Induce Stress Resistance in Nematode Caenorhabditis elegans

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