Association of Oxidative Stress Biomarkers and Antioxidant Enzymatic Activity in Male Infertility of North-East India

Mahanta, Rita; Gogoi, Adriana; Chaudhury, P. N. B.; Roy, Subhas Chandra; Bhattacharyya, I. K.; Sharma, Poonam

doi:10.1007/s13224-011-0070-3

Cited by 18 publications

(13 citation statements)

References 25 publications

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“…This excessive LPO impairs the antioxidant defence system in the male reproductive tract and leads to male infertility [72] . Results obtained in this investigation showed higher MDA concentration in the blood plasma of infertile men were similar with another Indian population study where blood and semen LPO levels were correlated positively [73] , hence smoking and alcohol exposure could be associated with elevated LPO levels. Shiva et al described that high MDA levels were associated with low sperm concentration, motility, morphology and related to poor sperm quality, hence sperm membrane integrity [23] .…”

Section: Resultssupporting

confidence: 89%

Impact of Smoking and Alcohol Consumption on Oxidative Status in Male Infertility and Sperm Quality

Ramgir¹,

Abilash²

2019

ijps

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Ramgir and Abilash: Smoking and Alcohol in Male Infertility The most common unhealthy lifestyle factors like cigarette smoking and drinking alcohol have controversial reports on how these impact male fertility. The present study assessed the potential effects of these habits on sperm quality, male reproductive hormones, and blood plasma oxidative profile in 133 infertile men with 124 fertile, healthy controls. Among the participants, 28 (10.8 %), 29 (11.2 %), 33 (12.8 %), 34 (13.2 %) fertile men were non-alcoholics and non-smokers (total abstainers), smokers, alcohol drinkers, and smokers plus alcohol drinkers, respectively. On the other hand, 32 (12.4 %), 30 (11.6 %), 34 (13.2 %), 37 (14.3 %) infertile men were total abstainers, smokers, alcohol drinkers, and smokers plus alcohol drinkers, respectively. The conventional sperm parameters and reproductive hormone levels were found to be significantly different in the subsets of infertile men than those of fertile men (p<0.001). In these subsets of infertile men, blood plasma malondialdehyde, nitric oxide concentrations were significantly higher and superoxide dismutase, reduced glutathione activities were significantly lower when compared to fertile men and infertile men total abstainers group (p<0.001). In infertile subjects, particularly smoking-alcohol drinking group was more affected than only smoking or alcohol drinking groups. Furthermore, these oxidative markers were correlated with sperm concentration, motility, and morphology, except nitric oxide concentration, which was only associated with sperm motility. These findings highlighted that blood plasma antioxidants are as efficient as seminal plasma antioxidants in male fertility evaluation.

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Section: Resultssupporting

confidence: 89%

Impact of Smoking and Alcohol Consumption on Oxidative Status in Male Infertility and Sperm Quality

Ramgir¹,

Abilash²

2019

ijps

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“…Another recent study demonstrated that catalase exerts a protective role to restore sperm quality (9,10). In agreement with these findings, some studies have reported the differential expression of antioxidant enzymes between fertile and infertile men (11)(12)(13). Consistent with these evidences, our previous proteomic study demonstrated that the sperm proteome changes during capacitation, which is one of the fundamental processes that spermatozoa undergo to acquire the ability to fertilize an egg (14).…”

Section: Introductionsupporting

confidence: 83%

Peroxiredoxin 4 as potential fertility marker in boars

Ryu

Pang

Rahman

et al. 2020

Preprint

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Background: High levels of reactive oxygen species are toxic to spermatozoa as they induce DNA damage, which leads to male infertility. Therefore, reduction of oxidative stress using reducing agents, such as antioxidant enzymes, is required. Peroxiredoxins (Prdxs) are known to play a critical role in the regulation of male fertility as antioxidant enzymes. Although several studies have suggested a close association between Prdxs and male fertility, few studies have explored the efficacy of Prdxs to predict fertility. Therefore, the current study was designed to discover the most sensitive biomarkers among the Prdxs with six isoforms, and furthermore to determine whether Prdxs are more suitable fertility markers for boar spermatozoa compared to conventional semen analysis.Results: The mRNA levels of PRDXs and several sperm parameters were examined in spermatozoa collected from 20 boars with different litter sizes. Our study showed that there was a significant positive correlation between the litter size and the levels of PRDX 4 among all isoforms in spermatozoa. Subsequently, a regression analysis using a combination of markers was conducted to increase efficacy for fertility prediction. According to the data, PRDX4 had the highest efficacy compared to other combination models. The prediction accuracy of male fertility was further evaluated through receiver operating characteristic curve analysis, which showed that PRDX 4 could predict the litter size with a high overall accuracy of 95%.Conclusions: As fertility biomarkers, genomic markers might be more accurate for predicting male fertility compared to conventional semen analysis. Our findings indicate that PRDX 4 might be a potential biomarker for diagnosing male fertility and subsequently improving reproductive efficacy in boars.

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“…In normozoospermic men, higher seminal activities of enzymatic antioxidants correlate with lower total malondialdehyde levels (MDA), indicating the protective effect of these antioxidants against oxidative stress-induced lipid peroxidation [ 133 , 134 ]. Conversely, infertile men with poor spermatozoa quality have lower levels of seminal enzymatic antioxidants that correspond with increased levels of lipid peroxidation [ 133 , 135 ].…”

Section: Antioxidants As Ameliorating Agentsmentioning

confidence: 99%

Utility of antioxidants during assisted reproductive techniques: an evidence based review

Agarwal

Durairajanayagam

Plessis

2014

Reprod Biol Endocrinol

181

134

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Assisted reproductive technology (ART) is a common treatment of choice for many couples facing infertility issues, be it due to male or female factor, or idiopathic. Employment of ART techniques, however, come with its own challenges as the in vitro environment is not nearly as ideal as the in vivo environment, where reactive oxygen species (ROS) build-up leading to oxidative stress is kept in check by the endogenous antioxidants system. While physiological amounts of ROS are necessary for normal reproductive function in vivo, in vitro manipulation of gametes and embryos exposes these cells to excessive ROS production either by endogenous or exogenous environmental factors. In this review, we discuss the sources of ROS in an in vitro clinical setting and the influence of oxidative stress on gamete/embryo quality and the outcome of IVF/ICSI. Sources of ROS and different strategies of overcoming the excessive generation of ROS in vitro are also highlighted. Endogenously, the gametes and the developing embryo become sources of ROS. Multiple exogenous factors act as potential sources of ROS, including exposure to visible light, composition of culture media, pH and temperature, oxygen concentration, centrifugation during spermatozoa preparation, ART technique involving handling of gamete/embryo and cryopreservation technique (freeze/thawing process). Finally, the use of antioxidants as agents to minimize ROS generation in the in vitro environment and as oral therapy is highlighted. Both enzymatic and non-enzymatic antioxidants are discussed and the outcome of studies using these antioxidants as oral therapy in the male or female or its use in vitro in media is presented. While results of studies using certain antioxidant agents are promising, the current body of evidence as a whole suggests the need for further well-designed and larger scale randomized controlled studies, as well as research to minimize oxidative stress conditions in the clinical ART setting.

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Association of Oxidative Stress Biomarkers and Antioxidant Enzymatic Activity in Male Infertility of North-East India

Cited by 18 publications

References 25 publications

Impact of Smoking and Alcohol Consumption on Oxidative Status in Male Infertility and Sperm Quality

Impact of Smoking and Alcohol Consumption on Oxidative Status in Male Infertility and Sperm Quality

Peroxiredoxin 4 as potential fertility marker in boars

Utility of antioxidants during assisted reproductive techniques: an evidence based review

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