Association of open field behavior with blood and semen characteristics in roosters: an alternative animal model

Ommati, Mohammad Mehdi; Heidari, Reza; Zamiri, Mohammad Javad; Shojaee, S.; Akhlaghi, A.; Sabouri, Samira

doi:10.1016/j.androl.2017.02.002

Cited by 10 publications

(10 citation statements)

References 26 publications

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“…In bulls, no correlation was found between serum T levels and sperm indices (Gábor et al ., ). No significant correlation was found between blood T and seminal characteristics in Iranian indigenous‏ ‏roosters (Ommati et al ., )‎. Zeman et al .…”

Section: Discussionmentioning

confidence: 98%

“…Epididymal sperm concentration was determined using a Neubauer hemocytometer under a light microscope at 200× magnification. Sperm viability and abnormality were evaluated in duplicate (200 sperm per slide) after eosin–nigrosin staining (Seed et al ., ; Ommati et al ., ). Spermatozoa without tail or head or with protoplasmic droplets were considered as abnormal.…”

Section: Methodsmentioning

confidence: 97%

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Retracted: Is immunosuppression, induced by neonatal thymectomy, compatible with poor reproductive performance in adult male rats?

et al. 2017

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With increasing knowledge that the immune system has a major impact on reproductive ‎health, the potential for cells arising in organs such as the thymus to alleviate oxidative stress ‎has been revealed. This study addresses the impact of neonatal thymectomy on male ‎reproductive function in pubertal and adult animals. Neonatal Sprague Dawley rats were allotted to four treatments consisting of fully thymectomized, partially thymectomized, intact, and sham-operated rats. Half of the rats in each treatment were sacrificed at 40 and the other half at 80 days of age. Testicular volume, ventral prostate and spleen weight, several sperm attributes (concentration, motility, livability, membrane integrity, sperm penetration into mucus, total antioxidant capacity, mitochondrial dehydrogenase activity), plasma superoxide dismutase, glutathione, and testosterone level as well as fertility decreased in thymectomized rats. Adrenal gland weight, sperm malondialdehyde level, indices of oxidative stress, sperm abnormality, testicular and sperm lipid peroxidation, protein carbonylation, and sperm reactive oxygen species generation increased in thymectomized rats. In thymectomized rats, the testes contained high levels of malondialdehyde but low levels of glutathione and ferric-reducing antioxidant power. Epididymal sperm reactive oxygen species, blood lipid peroxidation, and oxidative stress indices ‎‎in blood and spermatozoa were highest in fully thymectomized, intermediate ‎in partially thymectomized, and lowest in both pubertal and mature control rats. Blood levels of superoxide dismutase, lipid peroxidation indices, and ‎testosterone, and mitochondrial adenosine triphosphate and ‎dehydrogenase activities in epididymal spermatozoa were lowest in fully thymectomized, ‎intermediate in partially thymectomized, and highest in both pubertal and mature control rats.‎ The data indicated that increased oxidative stress and mitochondrial dysfunction might play a role in the mechanism of immunosuppression-induced testicular and sperm abnormalities.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 97%

Retracted: Is immunosuppression, induced by neonatal thymectomy, compatible with poor reproductive performance in adult male rats?

et al. 2017

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“…Sperm concentration was measured by transferring a portion of diluted epididymal fluid (10 µL) onto a Neubauer chamber and observing the cells under a light microscope (200 × magnification). The hypo-osmotic swelling (HOS) test, as a valid index of membrane integrity, was performed by mixing 10 µL of sperm suspension with 50 µL of hypo-osmotic solution (50-mOsm NaCl) for 10 min at 37 • C and calculating the percentages of spermatozoa (in at last 200 sperm per slide) with a swollen "bubble" around the curled flagellum, using light microscopy (1,000 × magnification) (31,56). Sperm abnormality and viability were measured in duplicate (200 sperm per sample) after eosin-nigrosin staining (20,31).…”

Section: Sperm Quality Evaluationmentioning

confidence: 99%

“…The hypo-osmotic swelling (HOS) test, as a valid index of membrane integrity, was performed by mixing 10 µL of sperm suspension with 50 µL of hypo-osmotic solution (50-mOsm NaCl) for 10 min at 37 • C and calculating the percentages of spermatozoa (in at last 200 sperm per slide) with a swollen "bubble" around the curled flagellum, using light microscopy (1,000 × magnification) (31,56). Sperm abnormality and viability were measured in duplicate (200 sperm per sample) after eosin-nigrosin staining (20,31). Spermatozoa with protoplasmic droplets, ab-axial tails, malformed heads, double tails, coiled tails, bent tails, and without tail or head were recorded as abnormal under a phase-contrast microscope (Olympus BX41; Olympus Optical Co. Ltd, Japan) (28,57).…”

Section: Sperm Quality Evaluationmentioning

confidence: 99%

“…Oxidative stress (OS), mitochondrial impairment, and their subsequent complications seem to play crucial roles in the xenobiotics-induced reprotoxicity and consequent infertility (20,21,(24)(25)(26)(27)(28)(29)(30). High sensitivity to OS, excessive reactive oxygen species (ROS) levels, and thiobarbituric acid reactive substances (TBARS) content could dramatically affect the functions of the reproductive organs (20,21,(26)(27)(28)(29)(30)(31)(32)(33)(34). For instance, oxidative injury and DNA damage studies indicated that high doses of MWCNT could cause DNA damage in murine sperm (35).…”

Section: Introductionmentioning

confidence: 99%

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Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.

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Glycine protects the male reproductive system against lead toxicity via alleviating oxidative stress, preventing sperm mitochondrial impairment, improving kinematics of sperm, and blunting the downregulation of enzymes involved in the steroidogenesis

Ommati

Ahmadi

Sabouri

et al. 2022

Environmental Toxicology

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Lead (Pb) is a highly toxic heavy metal widely dispersed in the environment because of human industrial activities. Many studies revealed that Pb could adversely affect several organs, including the male reproductive system. Pb‐induced reproductive toxicity could lead to infertility. Thus, finding safe and clinically applicable protective agents against this complication is important. It has been found that oxidative stress plays a fundamental role in the pathogenesis of Pb‐induced reprotoxicity. Glycine is the simplest amino acid with a wide range of pharmacological activities. It has been found that glycine could attenuate oxidative stress and mitochondrial impairment in various experimental models. The current study was designed to evaluate the role of glycine in Pb‐induced reproductive toxicity in male mice. Male BALB/c mice received Pb (20 mg/kg/day; gavage; 35 consecutive days) and treated with glycine (250 and 500 mg/kg/day; gavage; 35 consecutive days). Then, reproductive system weight indices, biomarkers of oxidative stress in the testis and isolated sperm, sperm kinetic, sperm mitochondrial indices, and testis histopathological alterations were monitored. A significant change in testis, epididymis, and Vas deferens weight was evident in Pb‐treated animals. Markers of oxidative stress were also significantly increased in the testis and isolated sperm of the Pb‐treated group. A significant disruption in sperm kinetic was also evident when mice received Pb. Moreover, Pb exposure caused significant deterioration in sperm mitochondrial indices. Tubular injury, tubular desquamation, and decreased spermatogenic index were histopathological alterations detected in Pb‐treated mice. It was found that glycine significantly blunted oxidative stress markers in testis and sperm, improved sperm mitochondrial parameters, causing considerable higher velocity‐related indices (VSL, VCL, and VAP) and percentages of progressively motile sperm, and decreased testis histopathological changes in Pb‐exposed animals. These data suggest glycine as a potential protective agent against Pb‐induced reproductive toxicity. The effects of glycine on oxidative stress markers and mitochondrial function play a key role in its protective mechanism.

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Association of open field behavior with blood and semen characteristics in roosters: an alternative animal model

Cited by 10 publications

References 26 publications

Retracted: Is immunosuppression, induced by neonatal thymectomy, compatible with poor reproductive performance in adult male rats?

Retracted: Is immunosuppression, induced by neonatal thymectomy, compatible with poor reproductive performance in adult male rats?

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Glycine protects the male reproductive system against lead toxicity via alleviating oxidative stress, preventing sperm mitochondrial impairment, improving kinematics of sperm, and blunting the downregulation of enzymes involved in the steroidogenesis

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