Association of NRAMP1 gene polymorphism with the productive traits of the Ukrainian Large White pig

Vashchenko, Pavlo; SAIENKO, ARTEM; Sukhno, V. V.; Tsereniuk, Oleksandr; Babicz, Marek; SHKAVRO, NATALYA; SMOŁUCHA, GRZEGORZ; ŁUSZCZEWSKA-SIERAKOWSKA, IWONA

doi:10.21521/mw.6698

Cited by 11 publications

(11 citation statements)

References 21 publications

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“…These breeds are threatened by high rates of consanguinity and the resulting loss of biodiversity (Llambí et al, 2020). At the same time, local breeds of pigs are distinguished by valuable qualities primarily related to their resistance to diseases and high adaptability (Vashchenko et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Comparative characteristics of polymorphisms of melanocortin 4 and ryanodine 1 receptor genes of Myrhorod pigs before and after the African swine fever outbreak

Tsereniuk,

Vashchenko,

Khokhlov

et al. 2023

Regul. Mech. Biosyst.

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One of the global problems that are anthropogenic in nature is the sharp decline in biodiversity, a special case of which is the reduction in the number of species of wild animals and the number of breeds of farm animals. The Myrhorod pig breed, due to its genetic and biological characteristics, is of great importance for the process of preserving the biodiversity of agricultural animals. The study’s object was Myrhorod breed pigs, the study focused on the variations in the allele frequency of the RYR1 / SNP g.1843 C>T and MC4R / SNP c.1426 G>A genes that occurred in the Myrhorod breed as it recovered from the African swine fever epidemic. The age at which one pig reaches 100 kg (days); the average daily weight gain from 90 days to slaughter (g); the thickness of the backfat (mm); the length of the carcass (cm); the cross-sectional area of the longest back muscle at the level of the 6th and 7th thoracic vertebrae (cm2); the slaughter output (%); and the yield of meat, fat, and bones from the carcass (%) are other quantitative changes in indicators of fattening and meat productivity. Significant alterations in the SNP marker MC4R polymorphism were observed during the restoration of the Myrhorod pig breed after the African swine fever epidemic. The frequency of the A allele dropped by 2.79 times to just 26.9% from 75.0%, which was 3.17 times higher than the frequency of the G allele until 2019. These alterations resulted from the forced crossing of the Myrhorod breed with the Pietrain breed, which has a high frequency of the G gene (83.0%), during the early phase of the breed's restoration in order to prevent close inbreeding. It is suggested that animals with the AA genotype should be preferred during further restoration in order to restore the Myrhorod breed to its original state. If crossbreeding becomes necessary again (to prevent excessive inbreeding), only breeds related to the Myrhorod (Berkshire, Poltava meat, Large White) should be used. The allele frequency in 2023 did not differ substantially from 2015 or 2019, based on the RYR1 DNA marker. The recessive T gene at the RYR1 g.1843 C>T locus was absent in 2023, confirming the stress resistance of the Myrhorod breed. When compared to the same indicator in the population of 2019, the average daily gain in the live weight of pigs belonging to the restored Myrhorod breed in 2023 was higher. Although this is a good development in terms of making profits by producing this breed of pig, these notable variations might point to important shifts in the polymorphism of genetic markers linked to growth rate. To determine the extent to which restoration has altered DNA markers linked to this productive attribute, more research is required to examine the frequency of alleles of genes linked to growth intensity.

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Section: Discussionmentioning

confidence: 99%

Comparative characteristics of polymorphisms of melanocortin 4 and ryanodine 1 receptor genes of Myrhorod pigs before and after the African swine fever outbreak

Tsereniuk,

Vashchenko,

Khokhlov

et al. 2023

Regul. Mech. Biosyst.

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“…He et al (2013) found a strong correlation between chicken resistance to S. enteritidis and a mutation at the NRAMP1 gene's g.24101991A>T location. Several studies have shown that the NRAMP1 gene is associated with tuberculosis in human populations (Li et al, 2022); Salmonella disease in rats (Janiszewski et al, 2021); Tuberculosis disease in cows (Yuan et al, 2021); The nature of pig immunity (Vashchenko et al, 2022); Salmonellosis and virus infections in goats and sheep (Worku, 2017).…”

Section: Introductionmentioning

confidence: 99%

Genetic Polymorphism of g.3768T>C loci of NRAMP1 gene in Kampung and KUB Chickens

Maskur,

Muhsinin,

Rozi

et al. 2024

JBT

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Natural Resistance-Associated Macrophage Protein 1 (NRAMP1) is a gene that regulates the body's resistance to illness. The genetic diversity of Kampung and KUB chicken population in Lombok island West Nusa Tenggara province was investigated based on g.3768T>C loci of NRAMP1 gene. Experimental animal consisted of 105 KUB and 67 Kampung chickens. The NRAMP1 gene polymorphisms in both population were identified using PCR-RFLP method. Genetic parameters of g.3768T>C loci were calculated using PopGene32. In this study, the locus g.3768T>C of the NRAMP1gene found three genotypes (TT, CT, CC) with the frequency of the C allele was higher than the T allele, respectively 0.709 and 0.210 in Kampung chicken, and 0.738 and 0.262 in KUB chicken. The mean value of observed heterozygosity (Ho) and expected heterozygosity (He) of g.3768T>C loci in both populations revealed low to medium genetic diversity, with the values 0.222 and 0.400, respectively. The mean value of observed heterozygosity is lower than expected, indicating that the population is heading towards a heterozygosity deficit and proving that the two populations are not in Hardy-Weinberg equilibrium. The mean PIC value of the NRAMP1 gene (g.3768T>C) in both chicken population was 0.319 and classified as fairly informative (0.50 > PIC > 0.25). Meanwhile, the value of the genetic differentiation index (Fst) for Kampung and KUB chicken population were 0.385 and 0.433, indicating that there is differentiation between two chicken population at the observed loci.

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“…Preliminary analysis of QTL genes' polymorphism is important for the expansion and development of methodological approaches to increasing the productivity of pigs (Buske et al, 2006;Bovo et al, 2020;Vashchenko et al, 2022). In the future, these approaches can be implemented on a large herd.…”

Section: Introductionmentioning

confidence: 99%

The effect of the ryanodine receptor gene on the reproductive traits of Welsh sows

Zhukorskyi¹,

Tsereniuk

Vashchenko

et al. 2022

Regul. Mech. Biosyst.

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The reproductive performance of sows largely determines the efficiency of the entire pig farming industry. The purpose of our work is the evaluation of polymorphism of the ryanodine receptor gene and its impact on the reproductive traits of sows of the Welsh breed of pigs. For this study, 148 pigs of the Welsh breed were used. The reproductive traits of sows were evaluated in two adjacent generations. We conducted a comprehensive assessment of the reproductive ability of sows using the SIRQS (selection index of reproductive qualities of sows), determined phenotypic consolidation coefficients and assessed the genetic potential of the animals’ productivity. The polymorphism of the RYR1 gene was determined using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP). Data processing was performed using single-factor analysis of variance (ANOVA). Polymorphism of the ryanodine receptor gene in sows of the Welsh pig breed was evaluated. 8.0% of the animals were identified as carriers of the mutant allele of the RYR1 gene. However, no homozygous RYR1-nn animals were found. Pigs of the maternal generation carrying the homozygous NN genotype had better reproductive performance in all indicators. Sows that were carriers of the mutant allele were characterized by lower values of the genetic productivity potential compared with the entire estimated population for all productive traits. Sows which were free of the mutant allele of the RYR1 gene were characterized by large values of the SIRQS index. The values of the coefficients of phenotypic consolidation of the number of live born piglets in sows without the mutant allele were lower than in sows with the mutant allele n. Better performance of sows free of the mutant allele of the RYR1 gene was established over sows carrying it in all evaluated traits of reproductive capacity (for different traits P ranged from 0.021 to 1.0*10–4), except for number of piglets born alive per sow in the daughter generation. Sows with the NN genotype had better selection index values by 15.7% in the maternal generation and by 10.2% in the daughter generation. In order to increase the reproductive ability of sows in the studied population of Welsh pigs and achieve similar results in other herds of this breed, animals free from the mutant allele of the RYR1 gene should be selected for further reproduction in the process of breeding, while on the contrary, carriers of this gene should be gradually eliminated from the herd. To carry out breeding work, further research is needed on the entire population of Welsh pigs for the RYR1 gene.

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Association of NRAMP1 gene polymorphism with the productive traits of the Ukrainian Large White pig

Cited by 11 publications

References 21 publications

Comparative characteristics of polymorphisms of melanocortin 4 and ryanodine 1 receptor genes of Myrhorod pigs before and after the African swine fever outbreak

Comparative characteristics of polymorphisms of melanocortin 4 and ryanodine 1 receptor genes of Myrhorod pigs before and after the African swine fever outbreak

Genetic Polymorphism of g.3768T>C loci of NRAMP1 gene in Kampung and KUB Chickens

The effect of the ryanodine receptor gene on the reproductive traits of Welsh sows

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