Association of neutrophils, lymphocytes, and neutrophil-lymphocyte ratio to overall mortality after endovascular abdominal aortic aneurysm repair

Nishibe, Toshiya; Kano, Masaki; Maekawa, Koki; Matsumoto, Ryumon; Fujiyoshi, Toshiki; Iwahashi, Toru; Kamiya, Kentaro; Ogino, Hitoshi

doi:10.23736/s0392-9590.22.04795-2

Cited by 3 publications

(5 citation statements)

References 27 publications

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“…The result indicated that these m1A regulators and their related genes were significantly associated with biological processes like neutrophil activation/neutrophil-mediated immunity, ncRNA metabolic process and regulation of cell cycle phase transition. Scientists have found that the number of neutrophils and neutrophil-lymphocyte ratio is not only correlated with in-hospital mortality in symptomatic unruptured AAA patients ( 34 ), but also the overall mortality after endovascular AAA repair ( 35 ). Therefore, m1A regulators and their related genes probably influence the prognosis of AAA via regulating the immune infiltration in AAA.…”

Section: Discussionmentioning

confidence: 99%

N1-Methyladenosine (m1A) Regulation Associated With the Pathogenesis of Abdominal Aortic Aneurysm Through YTHDF3 Modulating Macrophage Polarization

Jiang

Zhang

et al. 2022

Front. Cardiovasc. Med.

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ObjectivesThis study aimed to identify key AAA-related m1A RNA methylation regulators and their association with immune infiltration in AAA. Furthermore, we aimed to explore the mechanism that m1A regulators modulate the functions of certain immune cells as well as the downstream target genes, participating in the progression of AAA.MethodsBased on the gene expression profiles of the GSE47472 and GSE98278 datasets, differential expression analysis focusing on m1A regulators was performed on the combined dataset to identify differentially expressed m1A regulatory genes (DEMRGs). Additionally, CIBERSORT tool was utilized in the analysis of the immune infiltration landscape and its correlation with DEMRGs. Moreover, we validated the expression levels of DEMRGs in human AAA tissues by real-time quantitative PCR (RT-qPCR). Immunofluorescence (IF) staining was also applied in the validation of cellular localization of YTHDF3 in AAA tissues. Furthermore, we established LPS/IFN-γ induced M1 macrophages and ythdf3 knockdown macrophages in vitro, to explore the relationship between YTHDF3 and macrophage polarization. At last, RNA immunoprecipitation-sequencing (RIP-Seq) combined with PPI network analysis was used to predict the target genes of YTHDF3 in AAA progression.ResultsEight DEMRGs were identified in our study, including YTHDC1, YTHDF1-3, RRP8, TRMT61A as up-regulated genes and FTO, ALKBH1 as down-regulated genes. The immune infiltration analysis showed these DEMRGs were positively correlated with activated mast cells, plasma cells and M1 macrophages in AAA. RT-qPCR analysis also verified the up-regulated expression levels of YTHDC1, YTHDF1, and YTHDF3 in human AAA tissues. Besides, IF staining result in AAA adventitia indicated the localization of YTHDF3 in macrophages. Moreover, our in-vitro experiments found that the knockdown of ythdf3 in M0 macrophages inhibits macrophage M1 polarization but promotes macrophage M2 polarization. Eventually, 30 key AAA-related target genes of YTHDF3 were predicted, including CD44, mTOR, ITGB1, STAT3, etc.ConclusionOur study reveals that m1A regulation is significantly associated with the pathogenesis of human AAA. The m1A “reader,” YTHDF3, may participate in the modulating of macrophage polarization that promotes aortic inflammation, and influence AAA progression by regulating the expression of its target genes.

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Section: Discussionmentioning

confidence: 99%

N1-Methyladenosine (m1A) Regulation Associated With the Pathogenesis of Abdominal Aortic Aneurysm Through YTHDF3 Modulating Macrophage Polarization

Jiang

Zhang

et al. 2022

Front. Cardiovasc. Med.

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“…Patients were followed according to the institutional protocol, as reported previously. 5 Patients were observed through the database until death or the end of the follow-up period on September 30, 2021, whichever happened first. Postoperative outcomes included any causes of death.…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies, including ours, showed that a high neutrophil-to-lymphocyte ratio (NLR) is associated with poor long-term survival in patients undergoing EVAR for AAA. 2-5 The NLR of >3.0 to 4.0 was associated with increased mortality after elective EVAR, regardless of other comorbidities. Neutrophilia is associated with trauma, stress, bacterial infection, and cancer, any of which ultimately might increase the risk of death from any cause.…”

Section: Introductionmentioning

confidence: 98%

“…Likewise, lymphocytopenia is associated with overall mortality risk independently of traditional risk factors. 5 More recently, it has been reported that the lymphocyteto-monocyte ratio (LMR), a newly emerging inflammatory index, may have a potential value in predicting the prognosis of not only various malignancies 6 but also atherosclerotic cardiovascular diseases [7][8][9][10][11][12] ; both lymphocytes and monocytes and/or macrophages play an important role in the pathophysiology of atherosclerosis. 13 These studies suggest that the LMR can be a useful tool in discriminating between higher-risk and low-risk patients with atherosclerotic cardiovascular diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Likewise, lymphocytopenia is associated with overall mortality risk independently of traditional risk factors. 5…”

Section: Introductionmentioning

confidence: 99%

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The Preoperative Lymphocyte-To-Monocyte Ratio Predicts Mortality Among Patients Undergoing Endovascular Aortic Repair for Abdominal Aortic Aneurysm

Nishibe,

Kano,

Akiyama

et al. 2023

Vasc Endovascular Surg

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Purpose The purpose of this study was to investigate the preoperative lymphocyte-to-monocyte ratio (LMR) as a potential surrogate biomarker predictive of overall mortality in patients undergoing endovascular aortic repair (EVAR) for abdominal aortic aneurysm (AAA). Methods Data on patients with AAA treated by EVAR between March 2012 and December 2016 were obtained from a prospectively maintained EVAR database at Tokyo Medical University Hospital, Tokyo, Japan. The LMR was calculated by dividing the absolute lymphocyte count by the absolute monocyte count. Results One hundred seventy-six patients were included in this study after selection based on the exclusion criteria. The subjects consisted of 148 males and 28 females with a mean age of 78.5 years (range, 51-89 years). The median follow-up period was 4.98 years (range, .03-9.28). A receiver operating characteristic curve analysis determined the optimal cut-off value of the preoperative LMR for predicting overall mortality with 3.21 (area under the curve, .71; 95% confidence interval [CI], .62-.79; sensitivity, 57.4%; specificity, 77.0%; P < .001). On univariable and multivariable analyses, octogenarian (hazard ratio [HR], 1.89; 95%CI, 1.10-3.22; P = .020), poor nutritional status (HR, 2.95; 95%CI, 1.73-5.03; P < .001), chronic obstructive pulmonary disease (HR, 1.79; 95%CI, 1.06-3.03; P = .031), active cancer (HR, 2.60; 95%CI, 1.53-4.41; P < .001), and low preoperative LMR (HR, 2.56; 95%CI, 1.53-4.30; P < .001) were identified as independent predictors for overall mortality. Conclusion This study showed that a low preoperative LMR (<3.21) is an independent predictor of overall mortality after EVAR for AAA. The LMR may help in decision-making regarding the prediction of poor prognosis after EVAR.

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Curcumin mediates macrophage polarization to inhibit the formation of abdominal aortic aneurysms by inhibiting the expression of histone acetyltransferase EP300

Guo,

Ibrahim,

Zhang

et al. 2023

Arabian Journal of Chemistry

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Association of neutrophils, lymphocytes, and neutrophil-lymphocyte ratio to overall mortality after endovascular abdominal aortic aneurysm repair

Cited by 3 publications

References 27 publications

N1-Methyladenosine (m1A) Regulation Associated With the Pathogenesis of Abdominal Aortic Aneurysm Through YTHDF3 Modulating Macrophage Polarization

N1-Methyladenosine (m1A) Regulation Associated With the Pathogenesis of Abdominal Aortic Aneurysm Through YTHDF3 Modulating Macrophage Polarization

The Preoperative Lymphocyte-To-Monocyte Ratio Predicts Mortality Among Patients Undergoing Endovascular Aortic Repair for Abdominal Aortic Aneurysm

Curcumin mediates macrophage polarization to inhibit the formation of abdominal aortic aneurysms by inhibiting the expression of histone acetyltransferase EP300

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