Association of molecular markers derived from the BrCRISTO1 gene with prolycopene-enriched orange-colored leaves in Brassica rapa

Lee, Seohee; Lee, Sang-Choon; Byun, Dong Hae; Lee, Dong Hoon; Park, Jee Young; Lee, Jong Hoon; Lee, Hyun Oh; Sung, Sang Hyun; Yang, Tae-Jin

doi:10.1007/s00122-013-2209-3

Cited by 28 publications

(21 citation statements)

References 43 publications

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“…However, the BrCRTISO gene in different varieties with orange inner leaves exhibits significant difference in cDNA and genomic sequences [9, 10, 15]. For example, a 501 bp insertion was found at the 3′-end in the BrCRTISO gene of the 12-9 cultivar [15], while there is no such an insertion in the A21530 cultivar [10].…”

Section: Resultsmentioning

confidence: 99%

“…In previous studies, accumulation of carotenoid pigments was detected in the orange inner leaves, including prolycopene, lutein, proneurosporene, pro- ξ -carotene, ξ -carotene, 9- cis -b-carotene, β -carotene, and neurosporene [9–11, 15]. The accumulation of the carotenoid pigments is due to the expression changes of related genes caused by the inactivation of the BrCRTISO protein.…”

Section: Resultsmentioning

confidence: 99%

“…The color of the inner head leaves is usually white, yellow, or orange. The orange color of the inner leaves is a result of the accumulation of prolycopene and other carotenoid pigments [9–11]. In recent years, the orange head Chinese cabbage varieties have gradually captured the attention of breeders and consumers, since they have an attractive color and higher carotenoids compared with the white and yellow head cabbage.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the BrCRTISO (Bra031539) gene has been identified as the Br-or candidate by several research groups. Many insertions/deletions were identified in this gene [9–11, 15], resulting in mutant BrCRTISO proteins, which cannot catalyze the conversion from prolycopene to all- trans -lycopene [15]. Furthermore, studies have also shown mutations of the BrCRTISO gene dramatically alter the expressions of many other genes [11, 15].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapaL. ssp.pekinensis) and Molecular Marker Development

Zhang

Ding

et al. 2017

International Journal of Genomics

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Due to the visual appearance and high carotenoid content, orange inner leaves are a desirable trait for the Chinese cabbage. To understand the molecular mechanism underlying the formation of orange inner leaves, the BrCRTISO (Bra031539) gene, as the Br-or candidate gene, was analyzed among the white and orange varieties, and 7 single nucleotide polymorphisms (SNPs) were identified. However, only one SNP (C952 to T952) altered the amino acid sequence, resulting in a mutation from Leu318 to Phe318 in the orange varieties. Additionally, we analyzed differentially expressed genes (DEGs) between the orange and white F2 individuals (14-401 × 14-490) and found four downregulated genes were involved in the carotenoid biosynthesis pathway, which may lead to the accumulation of prolycopene and other carotenoid pigments in the orange inner leaves. In addition, we developed a novel InDel marker in the first intron, which cosegregates with the phenotypes of orange color inner leaves. In conclusion, these findings enhance our understanding of the underlying mechanism of pigment accumulation in the inner leaves of the Chinese cabbage. Additionally, the SNP (C952 to T952) and the InDel marker will facilitate the marker-assisted selection during Chinese cabbage breeding.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapaL. ssp.pekinensis) and Molecular Marker Development

Zhang

Ding

et al. 2017

International Journal of Genomics

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“…Based on the genome sequencing and comparative genomic study of B. rapa, the evolution of many gene families or categories such as circadian clock genes, 41 resistance genes, 42 stress response genes, [43][44][45] glucosinolate genes, 38 anthocyanin biosynthesis genes, 46 phytohormone-related genes, 47 certain transcript factor families 48 and other genes, [49][50][51][52][53][54] were accurately determined individually and studied in detail. Moreover, some functionally important genes related to self-incompatibility, 55,56 male sterility, 57 flowering regulation, 58-60 leaf heading 61 or color 62 have been identified or cloned, and functional studies have been conducted for some of these genes. These follow-up studies in B. rapa helped to further elucidate the evolution of specific genes after the WGT event.…”

Section: Two-step Theory To Illustrate the Wgt Process Inmentioning

confidence: 99%

Genome Triplication Drove the Diversification of Brassica Plants

Cheng¹,

Wu²,

Liang³

et al. 2015

Compendium of Plant Genomes

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The genus Brassica belongs to the plant family Brassicaceae, which includes many important crop species that are used as oilseed, condiments, or vegetables throughout the world. Brassica plants comprise many diverse species, and each species contains rich morphotypes showing extreme traits. Brassica species experienced an extra whole genome triplication (WGT) event compared with the model plant Arabidopsis thaliana. Whole genome sequencing of the Brassica species Brassica rapa, Brassica oleracea and others demonstrated that WGT plays an important role in the speciation and morphotype diversification of Brassica plants. Comparative genomic analysis based on the genome sequences of B. rapa and A. thaliana clearly identified the WGT event and further demonstrated that the translocated Proto-Calepine Karyotype (tPCK, n57) was the diploid ancestor of the three subgenomes in B. rapa. Following WGT, subsequent extensive genome fractionation, block reshuffling and chromosome reduction accompanied by paleocentromere descent from the three tPCK subgenomes during the rediploidization process produced stable diploid species. Genomic rearrangement of the diploid species and their hybridization then contributed to Brassica speciation. The subgenome dominance effect and biased gene retention, such as the over-retention of auxin-related genes after WGT, promoted functional gene evolution and thus propelled the expansion of rich morphotypes in the Brassica species. In conclusion, the WGT event initiated subsequent genomic and gene-level evolution, which further drove Brassica speciation and created rich morphotypes in each species. GENUS BRASSICA IN BRASSICACEAE Plants of the genus Brassica are grouped into the tribe Brassiceae, which belongs to the plant family Brassicaceae. Brassicaceae comprises a large family of plants that exhibit common and distinct features in their flowers. The flowers have cruciform petals and six stamens, two of which are short outer stamens. In total, Brassicaceae is composed of 3709 species and 338 genera, 1 with 308 of the 338 genera further assigned to 44 tribes.2 Among the abundant Brassicaceae species, the genus Brassica is important because it contains many economically valuable crops that are used as oilseeds, condiments, and culinary vegetables. Brassica species share an additional common feature in that they all experienced an extra whole genome triplication (WGT) event, which occurred approximately 9-15 million years ago 3,4 or even approximately 28 million years ago. 5-7THE U'S TRIANGLE MODEL DESCRIBES THE RELATIONSHIP AMONG BRASSICA CROPS Six species of the genus Brassica are used widely throughout the world as oilseed, condiments, fodder or vegetable crops. Three of these species are diploid (Brassica rapa, n510; B. nigra, n58; and B. oleracea, n59), whereas the other three are allotetraploids (B. juncea, n518; B. napus, n519; B. carinata, n517) derived from each pair of the three diploid species. The genetic relationships of these species were identified and confirmed by extensive experi...

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RETRACTED ARTICLE: BrRNE cleaves RNA in chloroplasts, regulating retrograde signals in Brassica rapa L. ssp. pekinensis

Zhang

et al. 2021

Theor Appl Genet

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Key message Brassica rapa RNE participates in the processing of polycistronic precursor transcripts into mature monocistronic mRNAs in plastids, thereby sending strong retrograde signals. Abstract Leaf color is one of the most important agronomic traits for Chinese cabbage. Not only is it closely linked to photosynthesis, thereby affecting plant growth, but it also influences consumer preference in the marketplace. A pale-green mutant rne was produced by EMS mutagenesis of Chinese cabbage inbred line A03. Chlorophyll content, photosynthetic rate, actual quantum efficiency (φPSII), and maximum quantum efficiency (Fv/Fm) of photosystem II (PSII) were all reduced in rne plants. Genetic analysis indicated that the pale-green trait was controlled by a pair of recessive alleles. Using mixed pool sequencing of F2 individuals derived from an rne × wild-type cross, we identified the essential gene Brassica rapa RNase E (BrRNE), which is responsible for chloroplast development. BrRNE cleaves polycistronic RNA in Chinese cabbage A03 plastids, but rne plants are defective in RNA processing and show reduced translation levels of the seven plastid genes, BrpsaB, BrpsaA, BrpsbA, BrpsbD, BrpsbB, BrpetA, and Brycf4. Abnormal RNA processing in the plastids sends retrograde signals that markedly regulate the expression of nuclear genes, upregulating genes that participate in ribosome and DNA replication pathways and repressing photosynthesis-associated nuclear genes (PhANGs). Our study reveals a new regulatory mechanism by which plastid RNA cleavage influences plastid development and leaf color, sending retrograde signals that affect the expression of nuclear genes in Brassica.

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Association of molecular markers derived from the BrCRISTO1 gene with prolycopene-enriched orange-colored leaves in Brassica rapa

Cited by 28 publications

References 43 publications

Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapaL. ssp.pekinensis) and Molecular Marker Development

Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapaL. ssp.pekinensis) and Molecular Marker Development

Genome Triplication Drove the Diversification of Brassica Plants

RETRACTED ARTICLE: BrRNE cleaves RNA in chloroplasts, regulating retrograde signals in Brassica rapa L. ssp. pekinensis

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